FGAM-synthase (NC_009334) Virus Tagged ORF Clone

CAT#: VC101013

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BNRF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129438



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CNY 19,950.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101013 represents NCBI reference of YP_001129438 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGACAGAGGGCGGGAGACTCAGATGCCCGTGGCCCGCTATGGTGGACCTTTCATTATGGTCAGGT
TGTTCGGCCAGGACGGCGAGGCCAATATCCAGGAACAGCGCCTTTACGAACTGCTGTCAGATCCAAGATC
AGCTCTCGGCTTGGACCCAGGACCACTGATCGCAGAAAACCTGTTGCTTGTGGCATTGCGCGGAACAAAT
AACGATCCCAGGCCACAACGACAGGAGCGCGCCCGAGAGCTGGCCTTGGTTGGGATACTCCTGGGCAACG
GTGAGCAGGGTGAGCACCTTGGAACCGAGTCCGCATTGGAAGCTAGTGGTAACAATTACGTCTATGCCTA
TGGGCCAGATTGGATGGCCCGGCCTAGCACATGGTCTGCTGAAATCCAACAGTTCCTGAGACTGCTCGGG
GCCACATACGTTCTGAGAGTAGAGATGGGTCGGCAGTTTGGCTTTGAAGTTCATAGATCACGCCCTTCCT
TTAGGCAGTTTCAAGCTATTAATCATCTCGTTCTTTTTGACAACGCACTGCGCAAATACGACTCTGGGCA
GGTTGCCGCCGGTTTTCAAAGAGCCCTGCTTGTCGCCGGACCAGAAACTGCCGACACCCGGCCAGATCTT
AGGAAACTTAACGAGTGGGTGTTCGGGGGTCGAGCTGCTGGAGGCCGCCAGCTCGCCGACGAACTCAAAA
TAGTCTCCGCACTGAGGGACACCTATTCCGGACACCTCGTCCTGCAGCCAACTGAAACACTGGACACCTG
GAAGGTGCTGTCTCGCGATACCAGAACAGCGCACTCCCTTGAACATGGTTTCATACACGCCGCCGGTACG
ATCCAGGCCAATTGTCCACAGCTGTTTATGAGAAGACAGCATCCGGGCCTCTTTCCGTTCGTGTCAGCTA
TAGCCAGCTCACTCGGGTGGTACTACCAGACGGCAACCGGTCCCGGCGCCGACGCACGCGCAGCAGCCAG
ACGCCAGCAAGCATTTCAAACACGAGCTGCCGCAGAGTGCCATGCAAAAAGTGGAGTCCCTGTTGTGGCA
GGCTTTTACAGGACTATCAACGCCACACTTAAGGGAGGCGAGGGCCTTCAGCCAACAATGTTCAACGGGG
AACTCGGTGCAATAAAGCACCAAGCCCTGGATACCGTTCGGTACGATTACGGGCACTATCTTATCATGCT
GGGTCCCTTTCAGCCTTGGTCCGGTTTGACTGCTCCACCTTGTCCATACGCAGAATCATCCTGGGCACAA
GCCGCAGTTCAGACTGCCCTTGAACTTTTTAGTGCGCTGTATCCAGCCCCTTGTATTTCAGGATACGCCA
GGCCCCCGGGACCCTCAGCTGTGATTGAACATCTGGGAAGCTTGGTTCCAAAAGGGGGTCTCCTTCTCTT
TCTCTCACACCTCCCAGACGACGTGAAGGACGGTCTGGGCGAGATGGGGCCAGCTCGAGCAACAGGGCCA
GGAATGCAACAGTTCGTGTCATCATATTTCCTCAACCCCGCCTGCAGCAATGTGTTCATCACCGTGCGAC
AAAGAGGAGAAAAAATTAATGGACGCACCGTACTGCAGGCCCTGGGAAGAGCCTGTGACATGGCAGGGTG
TCAGCACTACGTCCTGGGGAGTACCGTTCCCCTCGGTGGCCTGAATTTTGTGAATGATTTGGCGTCCCCT
GTGTCAACCGCGGAGATGATGGATGATTTCTCCCCCTTCTTTACTGTGGAATTTCCTCCCATTCAAGAAG
AAGGTGCCCGAAGCCCAGTTCCACTCGACGTGGACGAGAGCATGGACATCAGCCCTTCTTATGAACTCCC
ATGGCTGTCACTGGAGTCCTGTCTCACCTCCATACTCTCCCACCCCACCGTCGGCAGTAAAGAGCACTTG
GTACGACATACAGATCGCGTGTCAGGCGGCCGAGTGGCACAGCAACCCGGGGTGGGTCCCCTCGACCTCC
CCCTCGCTGATTACGCTTTCGTTGCCCACTCACAGGTCTGGACCAGGCCTGGCGGTGCCCCCCCACTTCC
ATACCGAACTTGGGACCGCATGACGGAAAAACTGCTCGTGTCCGCAAAGCCTGGGGGAGAGAACGTTAAG
GTGAGTGGCACTGTCATCACACTGGGGGAACAAGGCTATAAAGTATCCTTGGACCTCAGAGAGGGAACGC
GGCTGGCCATGGCCGAGGCGCTGCTTAACGCAGCTTTTGCCCCAATACTGGACCCCGAGGACGTACTCCT
GACCCTTCACTTGCACTTGGATCCACGCCGGGCGGACAATTCAGTGGTGATGGAAGCAATGACCGCCGCA
TCTGACTACGCTAGGGGCCTGGGCGTGAAGCTGACATTTGGGAGCGCAAGTTGTCCTGAAACCGGAAGCA
GTGCATCATCTTTTATGACAGTGGTCGCGTCAGTTTCAGCTCCAGGTGAGTTCTCTGGACCTCTGATCAC
ACCCGTGCTGCAAAAAACGGGTTCTTTGCTGATTGCTGTCCGCTGTGGAGACGGCAAAATCCAGGGTGGA
TCTCTTTTTGAGCAACTGTTCTCCGACGTCGCTACAACCCCCCGCGCACCAGAGGCCCTGTCCCTGAAAA
ATCTGTTTAGGGCCGTCCAGCAGCTGGTGAAGTCTGGAATTGTCTTGTCCGGACACGACATCTCCGATGG
CGGCCTCGTCACCTGTCTCGTGGAGATGGCCTTGGCAGGTCAGAGGGGAGTAACAATTACAATGCCCGTG
GCGAGCGACTATTTGCCCGAAATGTTTGCCGAACACCCGGGTTTGGTGTTCGAGGTGGAAGAGAGGTCTG
TGGGCGAGGTACTGCAGACTCTCAGATCCATGAATATGTACCCCGCAGTACTGGGCAGAGTGGGCGAGCA
AGGCCCAGATCAGATGTTCGAGGTTCAGCATGGACCCGAAACTGTGCTGCGCCAATCACTCCGACTTCTC
CTGGGAACATGGAGCAGCTTTGCCTCCGAGCAATACGAGTGCCTCCGACCTGACAGAATTAATCGCAGTA
TGCACGTCTCCGACTATGGATATAATGAAGCCCTGGCAGTTTCACCCTTGACTGGTAAAAATCTGAGCCC
TCGCAGACTGGTGACAGAGCCGGATCCAAGATGCCAGGTGGCCGTGCTGTGTGCTCCCGGGACAAGAGGA
CATGAGAGCCTGCTGGCCGCTTTCACAAACGCAGGTTGTCTTTGCCGCAGGGTGTTCTTCCGGGAGGTTC
GGGATAATACATTCCTGGACAAGTACGTCGGGCTCGCAATCGGAGGGGTGCACGGGGCTCGCGACTCAGC
CTTGGCGGGCAGAGCGACAGTCGCTCTGATAAATCGCTCTCCCGCCCTGAGAGACGCCATTCTGAAGTTC
CTGAACCGGCCGGACACTTTCAGTGTTGCCCTGGGCGAATTGGGGGTACAGGTCCTGGCAGGGCTTGGCG
CCGTGGGCTCAACCGACAATCCACCAGCCCCCGGGGTGGAGGTCAACGTACAGCGGAGCCCTTTGATACT
CGCTCCTAACGCCTCTGGAATGTTCGAGAGCAGGTGGCTGAACATCAGCATACCTGCCACCACTAGTAGC
GTTATGCTCCGGGGCCTCAGGGGATGCGTGCTGCCATGTTGGGTGCAGGGCTCATGTCTGGGCCTCCAGT
TTACCAACCTCGGTATGCCATATGTCCTCCAGAATGCCCACCAGATCGCGTGCCACTTTCATAGTAACGG
GACCGACGCCTGGCGCTTTGCGATGAATTATCCCAGAAACCCAACCGAACAGGGCAATATCGCGGGCCTC
TGCAGTAGGGATGGGAGGCATCTCGCTCTGCTGTGCGATCCGAGCCTGTGCACCGATTTTTGGCAGTGGG
AACATATTCCCCCCGCCTTCGGACACCCTACCGGCTGTAGCCCTTGGACTTTGATGTTTCAGGCAGCACA
CCTGTGGAGTCTCCGGCATGGGCGGCCATCCGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101013 representing YP_001129438
Red=Cloning sites Green=Tags

MEDRGRETQMPVARYGGPFIMVRLFGQDGEANIQEQRLYELLSDPRSALGLDPGPLIAENLLLVALRGTN
NDPRPQRQERARELALVGILLGNGEQGEHLGTESALEASGNNYVYAYGPDWMARPSTWSAEIQQFLRLLG
ATYVLRVEMGRQFGFEVHRSRPSFRQFQAINHLVLFDNALRKYDSGQVAAGFQRALLVAGPETADTRPDL
RKLNEWVFGGRAAGGRQLADELKIVSALRDTYSGHLVLQPTETLDTWKVLSRDTRTAHSLEHGFIHAAGT
IQANCPQLFMRRQHPGLFPFVSAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVA
GFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQ
AAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGP
GMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGLNFVNDLASP
VSTAEMMDDFSPFFTVEFPPIQEEGARSPVPLDVDESMDISPSYELPWLSLESCLTSILSHPTVGSKEHL
VRHTDRVSGGRVAQQPGVGPLDLPLADYAFVAHSQVWTRPGGAPPLPYRTWDRMTEKLLVSAKPGGENVK
VSGTVITLGEQGYKVSLDLREGTRLAMAEALLNAAFAPILDPEDVLLTLHLHLDPRRADNSVVMEAMTAA
SDYARGLGVKLTFGSASCPETGSSASSFMTVVASVSAPGEFSGPLITPVLQKTGSLLIAVRCGDGKIQGG
SLFEQLFSDVATTPRAPEALSLKNLFRAVQQLVKSGIVLSGHDISDGGLVTCLVEMALAGQRGVTITMPV
ASDYLPEMFAEHPGLVFEVEERSVGEVLQTLRSMNMYPAVLGRVGEQGPDQMFEVQHGPETVLRQSLRLL
LGTWSSFASEQYECLRPDRINRSMHVSDYGYNEALAVSPLTGKNLSPRRLVTEPDPRCQVAVLCAPGTRG
HESLLAAFTNAGCLCRRVFFREVRDNTFLDKYVGLAIGGVHGARDSALAGRATVALINRSPALRDAILKF
LNRPDTFSVALGELGVQVLAGLGAVGSTDNPPAPGVEVNVQRSPLILAPNASGMFESRWLNISIPATTSS
VMLRGLRGCVLPCWVQGSCLGLQFTNLGMPYVLQNAHQIACHFHSNGTDAWRFAMNYPRNPTEQGNIAGL
CSRDGRHLALLCDPSLCTDFWQWEHIPPAFGHPTGCSPWTLMFQAAHLWSLRHGRPSE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_009334
ORF Size 3954 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_009334.1, YP_001129438
RefSeq ORF 3954 bp
Locus ID 5176185
MW 142.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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