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E1B 55K (AC_000019) Virus Tagged ORF Clone
CAT＃: VC100075
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 35], codon optimized for human cell expression, AP_000573
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CNY 5,990.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100075 represents NCBI reference of AP_000573 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAGCCGACTCCTTCCAGCAGGGAATCCGCTTCGGTTTCCACTCCCACAGCATTGTGGAGAATA TGGAAGGCAGTCAAGATGAAGACAATCTGCGCCTGCTCGCTTCAGCCGCATTTGGATGCAGCGGGAACCC AGAAGCATCCACGGGCCATGCCAGTGGAAGTGGTGGTGGAACCGCCAGGGGGCAGCCTGAAAGTCGCCCC GGTCCATCTTCCGGTGGGGGCGGGGTGGCAGACCTTTCACCCGAACTTCAAAGAGTGCTCACCGGCAGCA CAAGTACCGGTCGGGATCGGGGTGTTAAACGCGAGCGCGCTAGCTCTGGTACGGATGCGCGGAGCGAGCT CGCTTTGTCTCTTATGAGCCGGAGAAGGCCCGAAACCATCTGGTGGCACGAAGTCCAAAAGGAGGGCCGG GATGAGGTATCTGTGCTGCAGGAGAAATATAGCCTCGAGCAGGTTAAAACGTGCTGGCTGGAGCCAGAAG ACGATTGGGCCGTGGCGATTAAAAATTATGCCAAGATAGCCCTGCGGCCCGATAAGCAGTACAAAATAAG CAGGAGGATTAACATCCGGAATGCCTGTTATATCTCTGGGAACGGTGCAGAGGTCGTCATCGACACACAG GATAAAACTGTGATCAGATGTTGCATGATGGATATGTGGCCAGGCGTCGTGGGGATGGAGGCCGTGACAT TCGTCAACGTCAAGTTTCGGGGTGACGGCTACAATGGCATCGTGTTCATGGCAAATACCAAACTTATCCT CCACGGATGCAGTTTTTTCGGCTTCAACAATACCTGCGTCGACGCCTGGGGTCAAGTGTCCGTGAGGGGT TGTTCCTTTTATGCTTGTTGGATTGCCACTGCCGGACGCACTAAGTCACAGCTGAGCTTGAAAAAATGTA TATTCCAACGGTGCAATCTCGGTATTCTGAATGAAGGAGAAGCCAGAGTGAGGCACTGTGCCTCAACCGA TACCGGATGTTTTATTTTGATCAAGGGCAACGCTTCCGTGAAGCACAACATGATCTGTGGTGCCAGTGAT GAGAGACCCTATCAAATGCTTACCTGCGCGGGTGGTCACTGCAACATGCTTGCCACCGTGCATATCGTAT CACACCAGCGCAAGAAGTGGCCAGTATTCGACCACAATGTTCTCACGAAATGTACGATGCACGCTGGAGG GAGGCGAGGGATGTTTATGCCTTACCAGTGCAATATGAACCACGTGAAAGTCCTCCTTGAGCCAGATGCC TTCAGCAGAATGTCTCTGACAGGAATATTCGACATGAACACTCAGATCTGGAAAATCCTGAGGTACGACG ATACCAGATCAAGAGTGCGGGCCTGTGAATGTGGTGGGAAGCACGCGAGGTTTCAGCCCGTCTGCGTAGA TGTAACCGAAGATCTGAGACCCGATCACCTGGTGATCGCGAGGACAGGGGCTGAATTCGGCAGCAGCGGT GAAGAGACTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100075 representing AP_000573
Red=Cloning sites Green=Tags MDPADSFQQGIRFGFHSHSIVENMEGSQDEDNLRLLASAAFGCSGNPEASTGHASGSGGGTARGQPESRP GPSSGGGGVADLSPELQRVLTGSTSTGRDRGVKRERASSGTDARSELALSLMSRRRPETIWWHEVQKEGR DEVSVLQEKYSLEQVKTCWLEPEDDWAVAIKNYAKIALRPDKQYKISRRINIRNACYISGNGAEVVIDTQ DKTVIRCCMMDMWPGVVGMEAVTFVNVKFRGDGYNGIVFMANTKLILHGCSFFGFNNTCVDAWGQVSVRG CSFYACWIATAGRTKSQLSLKKCIFQRCNLGILNEGEARVRHCASTDTGCFILIKGNASVKHNMICGASD ERPYQMLTCAGGHCNMLATVHIVSHQRKKWPVFDHNVLTKCTMHAGGRRGMFMPYQCNMNHVKVLLEPDA FSRMSLTGIFDMNTQIWKILRYDDTRSRVRACECGGKHARFQPVCVDVTEDLRPDHLVIARTGAEFGSSG EETD TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | AC_000019 |
| ORF Size | 1482 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | AC_000019.1, AP_000573 |
| RefSeq ORF | 1482 bp |
| MW | 54.6 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100073 | Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 35], codon optimized for human cell expression, AP_000571 |
CNY 3,800.00 |
|
| VC100074 | Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus 35], codon optimized for human cell expression, AP_000572 |
CNY 3,800.00 |
|
| VC100076 | Myc-DDK-tagged ORF clone of viral ORF for IX [Human adenovirus 35], codon optimized for human cell expression, AP_000574 |
CNY 3,800.00 |
|
| VC100077 | Myc-DDK-tagged ORF clone of viral ORF for IVa2 [Human adenovirus 35], codon optimized for human cell expression, AP_000575 |
CNY 5,510.00 |
|
| VC100078 | Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 35], codon optimized for human cell expression, AP_000576 |
CNY 18,050.00 |
|
| VC100079 | Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 35], codon optimized for human cell expression, AP_000577 |
CNY 7,890.00 |
|
| VC100080 | Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 35], codon optimized for human cell expression, AP_000578 |
CNY 4,660.00 |
|
| VC100081 | Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus 35], codon optimized for human cell expression, AP_000579 |
CNY 7,030.00 |
|
| VC100082 | Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 35], codon optimized for human cell expression, AP_000580 |
CNY 6,750.00 |
|
| VC100083 | Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 35], codon optimized for human cell expression, AP_000581 |
CNY 3,800.00 |
|
| VC100084 | Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 35], codon optimized for human cell expression, AP_000582 |
CNY 4,180.00 |
|
| VC100085 | Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus 35], codon optimized for human cell expression, AP_000583 |
CNY 3,800.00 |
|
| VC100086 | Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus 35], codon optimized for human cell expression, AP_000584 |
CNY 3,800.00 |
|
| VC100087 | Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 35], codon optimized for human cell expression, AP_000585 |
CNY 14,350.00 |
|
| VC100088 | Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus 35], codon optimized for human cell expression, AP_000586 |
CNY 3,800.00 |
|
| VC100089 | Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 35], codon optimized for human cell expression, AP_000587 |
CNY 6,270.00 |
|
| VC100090 | Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 35], codon optimized for human cell expression, AP_000588 |
CNY 12,350.00 |
|
| VC100091 | Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 35], codon optimized for human cell expression, AP_000589 |
CNY 3,800.00 |
|
| VC100092 | Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 35], codon optimized for human cell expression, AP_000590 |
CNY 3,800.00 |
|
| VC100093 | Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 35], codon optimized for human cell expression, AP_000591 |
CNY 3,800.00 |
|
| VC100094 | Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus 35], codon optimized for human cell expression, AP_000592 |
CNY 3,800.00 |
|
| VC100095 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha0 [Human adenovirus 35], codon optimized for human cell expression, AP_000593 |
CNY 3,800.00 |
|
| VC100096 | Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus 35], codon optimized for human cell expression, AP_000594 |
CNY 3,800.00 |
|
| VC100097 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus 35], codon optimized for human cell expression, AP_000595 |
CNY 3,800.00 |
|
| VC100098 | Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-gamma1 [Human adenovirus 35], codon optimized for human cell expression, AP_000596 |
CNY 3,800.00 |
|
| VC100099 | Myc-DDK-tagged ORF clone of viral ORF for E3 RID-alpha [Human adenovirus 35], codon optimized for human cell expression, AP_000597 |
CNY 3,800.00 |
|
| VC100100 | Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 35], codon optimized for human cell expression, AP_000598 |
CNY 3,800.00 |
|
| VC100101 | Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus 35], codon optimized for human cell expression, AP_000599 |
CNY 3,800.00 |
|
| VC100102 | Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus 35], codon optimized for human cell expression, AP_000600 |
CNY 3,800.00 |
|
| VC100103 | Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus 35], codon optimized for human cell expression, AP_000601 |
CNY 3,800.00 |
|
| VC100104 | Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 35], codon optimized for human cell expression, AP_000603 |
CNY 3,800.00 |
|
| VC100105 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 35], codon optimized for human cell expression, AP_000602 |
CNY 3,800.00 |
|
| VC100106 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus 35], codon optimized for human cell expression, AP_000604 |
CNY 3,800.00 |
|
| VC100107 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus 35], codon optimized for human cell expression, AP_000605 |
CNY 3,800.00 |
|
| VC100108 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF2 [Human adenovirus 35], codon optimized for human cell expression, AP_000606 |
CNY 3,800.00 |
|
| VC100109 | Myc-DDK-tagged ORF clone of viral ORF for E4 ORF1 [Human adenovirus 35], codon optimized for human cell expression, AP_000607 |
CNY 3,800.00 |
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