CUL4A (NM_001278514) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC337163 representing NM_001278514. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTCTACAAGCAACTGCGTCAGGCCTGTGAAGACCACGTCCAGGCACAGATCCTTCCGTTTAGAGAA GACTCACTAGATAGTGTTTTATTTTTAAAGAAGATTAACACGTGCTGGCAGGACCACTGCAGACAAATG ATCATGATCAGAAGCATCTTCCTGTTCTTGGACCGCACCTATGTGCTGCAGAACTCCACGCTGCCCTCC ATCTGTCCTGTTTCTTACTGTCTATACAGGGATATGGGATTAGAACTGTTTAGAACCCATATTATTAGT GATAAAATGGTTCAGAGTAAAACCATTGATGGAATCCTACTGCTGATCGAGCGCGAGAGGAGCGGCGAG GCCGTGGACCGGAGCCTGTTGCGGAGCCTCCTGGGCATGCTGTCTGACCTGCAGGTGTATAAAGATTCA TTTGAACTGAAATTTTTGGAAGAGACTAATTGCTTATATGCTGCCGAAGGCCAAAGGTTAATGCAGGAA AGAGAGGTTCCAGAATATCTTAACCATGTAAGTAAACGCTTAGAGGAAGAGGGAGACAGAGTAATCACT TACTTGGACCACAGCACACAGAAACCACTGATTGCTTGTGTGGAGAAACAGCTATTAGGAGAACATTTA ACAGCAATTCTGCAGAAAGGGCTCGACCACTTACTGGATGAGAACAGAGTGCCGGACCTCGCACAGATG TACCAGCTGTTCAGCCGGGTGAGGGGCGGGCAGCAGGCGCTGCTGCAGCACTGGAGCGAGTACATCAAG ACTTTTGGAACAGCGATCGTAATCAATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGTTGGACTTC AAGGACAAGGTGGACCACGTGATCGAGGTCTGCTTCCAGAAGAATGAGCGGTTCGTCAACCTGATGAAG GAGTCCTTTGAGACGTTCATCAACAAGAGACCCAACAAGCCTGCAGAACTGATCGCAAAGCATGTGGAT TCAAAGTTAAGAGCAGGCAACAAAGAAGCCACAGACGAGGAGCTGGAGCGGACGTTGGACAAGATCATG ATCCTGTTCAGGTTTATCCACGGTAAAGATGTCTTTGAAGCATTTTATAAAAAAGATTTGGCAAAAAGA CTCCTTGTTGGGAAAAGTGCCTCAGTCGATGCTGAAAAGTCTATGTTGTCAAAGCTCAAGCATGAGTGC GGTGCAGCCTTCACCAGCAAGCTGGAAGGCATGTTCAAGGACATGGAGCTTTCGAAGGACATCATGGTT CATTTCAAGCAGCATATGCAGAATCAGAGTGACTCAGGCCCTATAGACCTCACAGTGAACATACTCACA ATGGGCTACTGGCCAACATACACGCCCATGGAAGTGCACTTAACCCCAGAAATGATTAAACTTCAGGAA GTATTTAAGGCATTTTATCTTGGAAAGCACAGTGGTCGAAAACTTCAGTGGCAAACTACTTTGGGACAT GCTGTTTTAAAAGCGGAGTTTAAAGAAGGGAAGAAGGAATTCCAGGTGTCCCTCTTCCAGACACTGGTG CTCCTCATGTTCAACGAGGGAGATGGCTTCAGCTTTGAGGAGATAAAAATGGCCACGGGGATAGAGGAT AGTGAATTGCGCAGAACGCTGCAGTCCCTGGCCTGTGGCAAAGCACGTGTGCTGATTAAAAGTCCCAAA GGAAAGGAAGTGGAAGATGGAGACAAGTTCATTTTTAATGGAGAGTTCAAGCACAAGTTGTTTAGAATA AAGATCAATCAAATTCAGATGAAGGAAACTGTTGAGGAACAGGTTAGCACCACTGAGAGAGTGTTTCAG GATAGACAATATCAGATTGATGCTGCTATCGTCAGAATAATGAAGATGAGAAAGACTCTTGGTCATAAT CTTCTAGTTTCTGAATTATATAATCAGCTGAAATTTCCAGTAAAGCCTGGAGATTTGAAAAAGAGAATT GAATCTCTGATAGACAGAGACTATATGGAGAGAGACAAAGACAATCCGAATCAGTACCACTACGTGGCC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278514
Insert Size	2004 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278514.1
RefSeq Size	3721 bp
RefSeq ORF	2004 bp
Locus ID	8451
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis
MW	77.7 kDa
Gene Summary	CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009] Transcript Variant: This variant (4) utilizes an alternate 5' terminal exon and an alternate in-frame splice junction, compared to variant 1. This results in an isoform (3) with a shorter N-terminus and an alternate internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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