CUL4A (NM_001278514) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239269 representing NM_001278514 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTACAAGCAACTGCGTCAGGCCTGTGAAGACCACGTCCAGGCACAGATCCTTCCGTTTAGAGAAG ACTCACTAGATAGTGTTTTATTTTTAAAGAAGATTAACACGTGCTGGCAGGACCACTGCAGACAAATGAT CATGATCAGAAGCATCTTCCTGTTCTTGGACCGCACCTATGTGCTGCAGAACTCCACGCTGCCCTCCATC TGTCCTGTTTCTTACTGTCTATACAGGGATATGGGATTAGAACTGTTTAGAACCCATATTATTAGTGATA AAATGGTTCAGAGTAAAACCATTGATGGAATCCTACTGCTGATCGAGCGCGAGAGGAGCGGCGAGGCCGT GGACCGGAGCCTGTTGCGGAGCCTCCTGGGCATGCTGTCTGACCTGCAGGTGTATAAAGATTCATTTGAA CTGAAATTTTTGGAAGAGACTAATTGCTTATATGCTGCCGAAGGCCAAAGGTTAATGCAGGAAAGAGAGG TTCCAGAATATCTTAACCATGTAAGTAAACGCTTAGAGGAAGAGGGAGACAGAGTAATCACTTACTTGGA CCACAGCACACAGAAACCACTGATTGCTTGTGTGGAGAAACAGCTATTAGGAGAACATTTAACAGCAATT CTGCAGAAAGGGCTCGACCACTTACTGGATGAGAACAGAGTGCCGGACCTCGCACAGATGTACCAGCTGT TCAGCCGGGTGAGGGGCGGGCAGCAGGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACTTTTGGAAC AGCGATCGTAATCAATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGTTGGACTTCAAGGACAAGGTG GACCACGTGATCGAGGTCTGCTTCCAGAAGAATGAGCGGTTCGTCAACCTGATGAAGGAGTCCTTTGAGA CGTTCATCAACAAGAGACCCAACAAGCCTGCAGAACTGATCGCAAAGCATGTGGATTCAAAGTTAAGAGC AGGCAACAAAGAAGCCACAGACGAGGAGCTGGAGCGGACGTTGGACAAGATCATGATCCTGTTCAGGTTT ATCCACGGTAAAGATGTCTTTGAAGCATTTTATAAAAAAGATTTGGCAAAAAGACTCCTTGTTGGGAAAA GTGCCTCAGTCGATGCTGAAAAGTCTATGTTGTCAAAGCTCAAGCATGAGTGCGGTGCAGCCTTCACCAG CAAGCTGGAAGGCATGTTCAAGGACATGGAGCTTTCGAAGGACATCATGGTTCATTTCAAGCAGCATATG CAGAATCAGAGTGACTCAGGCCCTATAGACCTCACAGTGAACATACTCACAATGGGCTACTGGCCAACAT ACACGCCCATGGAAGTGCACTTAACCCCAGAAATGATTAAACTTCAGGAAGTATTTAAGGCATTTTATCT TGGAAAGCACAGTGGTCGAAAACTTCAGTGGCAAACTACTTTGGGACATGCTGTTTTAAAAGCGGAGTTT AAAGAAGGGAAGAAGGAATTCCAGGTGTCCCTCTTCCAGACACTGGTGCTCCTCATGTTCAACGAGGGAG ATGGCTTCAGCTTTGAGGAGATAAAAATGGCCACGGGGATAGAGGATAGTGAATTGCGCAGAACGCTGCA GTCCCTGGCCTGTGGCAAAGCACGTGTGCTGATTAAAAGTCCCAAAGGAAAGGAAGTGGAAGATGGAGAC AAGTTCATTTTTAATGGAGAGTTCAAGCACAAGTTGTTTAGAATAAAGATCAATCAAATTCAGATGAAGG AAACTGTTGAGGAACAGGTTAGCACCACTGAGAGAGTGTTTCAGGATAGACAATATCAGATTGATGCTGC TATCGTCAGAATAATGAAGATGAGAAAGACTCTTGGTCATAATCTTCTAGTTTCTGAATTATATAATCAG CTGAAATTTCCAGTAAAGCCTGGAGATTTGAAAAAGAGAATTGAATCTCTGATAGACAGAGACTATATGG AGAGAGACAAAGACAATCCGAATCAGTACCACTACGTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239269 representing NM_001278514 Red=Cloning site Green=Tags(s) MLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSI CPVSYCLYRDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFE LKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAI LQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKV DHVIEVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRF IHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHM QNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEF KEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGD KFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQ LKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278514
ORF Size	2001 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278514.2
RefSeq Size	3721 bp
RefSeq ORF	2004 bp
Locus ID	8451
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis
MW	78.2 kDa
Gene Summary	CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009]

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