UBOX5 (NM_001267584) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hUIP5; RNF37; UBCE7IP5; UIP5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336543 representing NM_001267584. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCAGCTGATGGT TACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGTTTCAGGACAGAGTATTTC ATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAATGTGGAAATCTGTAGGATCAACATAGAC CTCACAGCTGGGGGAGGTCAGAACGTCACTGGCCTGGAAATGTACACATCTGCCTCATCTAGCAGAGTG TCTTGGAATACGCCCCAGTGCCGGACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTC ACCTTGGTAGGCAAAGTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCC AGGCCCCCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGGAAT AAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTGACAGGCGGCGGT ATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACCTGCTCCCAGGAAGTGATAGAC AGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGATGTGGCTCTGCAGGCTCCAGCCTTGCCCATG GAAAGTGACTGTGACCCTGGGGACCAGCCTGAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCC GAGATCATTCAGGATGTGCCTGAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATG CTGCTGCCCTCAGGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGG GGCCGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCTCACCCC TCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGCCACCTGCTTGGGAGA GCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCCTCTCAGAAAAGGAAGATAGAGCAG GCTGAACATGTCCCAGACAGTAACTTTGGTGTAAATGCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTC TTACCCACTACCTCAGAGCACACTGCTAAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATG GACTGCTCGACAGGTCCACTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCC ACCCTTGGCTCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGCCTGGGAGCATCCTGGGCCCCGAATGTGCCTC CTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTACCAGCTGCCCTGCGGCCACCTCCT GTGCCGACCCTGCCTGGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001267584
Insert Size	1539 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267584.1
RefSeq Size	4357 bp
RefSeq ORF	1539 bp
Locus ID	22888
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	55.5 kDa
Gene Summary	This gene encodes a U-box domain containing protein. The encoded protein interacts with E2 enzymes and may play a role in the ubiquitination pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, compared to variant 1, which results in a translational frameshift, compared to variant 1. The resulting isoform (c) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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