UBOX5 (NM_001267584) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	hUIP5; RNF37; UBCE7IP5; UIP5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238649 representing NM_001267584 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCAGCTGATGGTT ACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGTTTCAGGACAGAGTATTTCAT TAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAATGTGGAAATCTGTAGGATCAACATAGACCTC ACAGCTGGGGGAGGTCAGAACGTCACTGGCCTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTT GGAATACGCCCCAGTGCCGGACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTT GGTAGGCAAAGTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGGAATAAAGGGG CTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTGACAGGCGGCGGTATCCCTTG TATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACCTGCTCCCAGGAAGTGATAGACAGCATCCTG CTGGTCACCTCAGAGAACCTGCCTCAGGATGTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACT GTGACCCTGGGGACCAGCCTGAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCA GGATGTGCCTGAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGCCGAGTGCCCA GTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCTCACCCCTCCCTCAAGGCCCG GATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGCCACCTGCTTGGGAGAGCACAGACGGCATTG GCAGTGATCCCTTCTTCCATTGTTCTGCCCTCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAG ACAGTAACTTTGGTGTAAATGCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGA GCACACTGCTAAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGCTCTATGCCCT CCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGAGGGAGCAACACTTCCTGGAG GCCTGGCACCGGCTCGGCCTGGGAGCATCCTGGGCCCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCC CTACTTCAAAAAGGAGCCGGTGTACCAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238649 representing NM_001267584 Red=Cloning site Green=Tags(s) MVINLCLPQFRPRIHCNKISADGYEVENLISEDLTKRSHGFRTEYFIKPPVYVTVSFPFNVEICRINIDL TAGGGQNVTGLEMYTSASSSRVSWNTPQCRTLGPAEPSVPDKEAFTLVGKVLLKNQSQVVFSHRGFKARP PFGAMEATLPSPAVVAQELWNKGALSLSHVAHLRICITHVTGGGIPCIKRLEVWGQPAKTCSQEVIDSIL LVTSENLPQDVALQAPALPMESDCDPGDQPESQQAPSSLQKLAEIIQDVPEEFLDPITLEIMPCPMLLPS GKVIDQSTLEKCNRSEATWGRVPSDPFTGVAFTPHSQPLPHPSLKARIDHFLLQHSIPGCHLLGRAQTAL AVIPSSIVLPSQKRKIEQAEHVPDSNFGVNASCFSATSPLVLPTTSEHTAKKMKATNEPSLTHMDCSTGP LSHEQKLSQSLEIALASTLGSMPSFTARLTRGQLQHLGTRGSNTSWRPGTGSAWEHPGPRMCLLQKSIFS LLQKGAGVPAALRPPPVPTLPG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001267584
ORF Size	1536 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267584.2
RefSeq Size	4357 bp
RefSeq ORF	1539 bp
Locus ID	22888
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	55.9 kDa
Gene Summary	This gene encodes a U-box domain containing protein. The encoded protein interacts with E2 enzymes and may play a role in the ubiquitination pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

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