SMAP1 (NM_001281439) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SMAP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336119 representing NM_001281439. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGACGCGCTCCTGTCGGGAGAAGGCTCAGAAGCTGAACGAGCAGCACCAGCTCATCCTATCCAAG CTTCTGAGGGAGGAGGACAACAAGTACTGCGCCGACTGCGAGGCCAAAGGTCCTCGATGGGCTTCCTGG AATATTGGTGTGTTTATTTGCATCAGATGTGCTGGAATTCATAGAAATCTTGGGGTTCATATATCCAGG GTCAAATCAGTCAACCTAGACCAATGGACAGCAGAACAGATACAGTGCATGCAAGATATGGGAAATACT AAAGCAAGACTACTCTATGAAGCCAATCTTCCAGAGAACTTTCGAAGACCACAGACAGATCAAGCAGTG GAATTTTTCATCAGAGATAAATATGAAAAGAAGAAATACTACGATAAAAATGCCATAGCTATTACAAAT AAAGAAAAGGAAAAAAAAAAGGAAGAGAAAAAGAGAGAAAAGGAGCCAGAAAAGCCGGCAAAACCACTT ACAGCTGAAAAGCTGCAGAAGAAAGATCAGCAACTGGAGCCTAAAAAAAGTACCAGCCCTAAAAAAGCT GCGGAGCCCACTGTGGATCTTTTAGGACTTGATGGCCCTGCTGTGGCACCAGTGACCAACGGGAACACA ACGGTGCCACCCCTGAACGATGATCTGGACATCTTTGGACCGATGATTTCTAATCCCTTACCTGCAACT GTCATGCCCCCAGCTCAGGGGACACCCTCTGCACCAGCAGCTGCAACCCTGTCTACAGTAACATCTGGG GATCTAGATTTATTCACTGAGCAAACTACAAAATCAGAAGAAGTGGCAAAGAAACAACTTTCCAAAGAC TCCATCTTATCTCTGTATGGCACAGGAACCATTCAACAGCAAAGTACTCCTGGTGTATTTATGGGACCC ACAAATATACCATTTACCTCACAAGCACCAGCTGCATTTCAGGGCTTTCCATCGATGGGCGTGCCTGTG CCTGCAGCTCCTGGCCTTATAGGAAATGTGATGGGACAGAGTCCAAGCATGATGGTGGGCATGCCCATG CCCAATGGGTTTATGGGAAATGCACAAACTGGTGTGATGCCACTTCCTCAGAACGTTGTTGGCCCCCAA GGAGGAATGGTGGGACAAATGGGTGCACCCCAGAGTAAGTTTGGCCTGCCGCAAGCTCAGCAGCCCCAG TGGAGCCTCTCACAGATAATGCAGAAGGGTGATGCTGTTCTCCAGCACTCCATCAGTGCAATCTACTGG CCAATGACAAGGTGGTTAAAATGTCCTTTAGTAGATGAATCAGCAGATGGCTGGCATGAGTATCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281439
Insert Size	1311 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281439.1
RefSeq Size	3356 bp
RefSeq ORF	1311 bp
Locus ID	60682
UniProt ID	Q8IYB5
Protein Pathways	Endocytosis
MW	47.9 kDa
Gene Summary	The protein encoded by this gene is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) has multiple coding region differences, compared to variant 1, one of which results in a frameshift. The resulting protein (isoform C) has a distinct C-terminus and is shorter than isoform A.

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