SMAP1 (NM_001281439) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SMAP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238225 representing NM_001281439 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGCGCTCCTGTCGGGAGAAGGCTCAGAAGCTGAACGAGCAGCACCAGCTCATCCTATCCAAGC TTCTGAGGGAGGAGGACAACAAGTACTGCGCCGACTGCGAGGCCAAAGGTCCTCGATGGGCTTCCTGGAA TATTGGTGTGTTTATTTGCATCAGATGTGCTGGAATTCATAGAAATCTTGGGGTTCATATATCCAGGGTC AAATCAGTCAACCTAGACCAATGGACAGCAGAACAGATACAGTGCATGCAAGATATGGGAAATACTAAAG CAAGACTACTCTATGAAGCCAATCTTCCAGAGAACTTTCGAAGACCACAGACAGATCAAGCAGTGGAATT TTTCATCAGAGATAAATATGAAAAGAAGAAATACTACGATAAAAATGCCATAGCTATTACAAATAAAGAA AAGGAAAAAAAAAAGGAAGAGAAAAAGAGAGAAAAGGAGCCAGAAAAGCCGGCAAAACCACTTACAGCTG AAAAGCTGCAGAAGAAAGATCAGCAACTGGAGCCTAAAAAAAGTACCAGCCCTAAAAAAGCTGCGGAGCC CACTGTGGATCTTTTAGGACTTGATGGCCCTGCTGTGGCACCAGTGACCAACGGGAACACAACGGTGCCA CCCCTGAACGATGATCTGGACATCTTTGGACCGATGATTTCTAATCCCTTACCTGCAACTGTCATGCCCC CAGCTCAGGGGACACCCTCTGCACCAGCAGCTGCAACCCTGTCTACAGTAACATCTGGGGATCTAGATTT ATTCACTGAGCAAACTACAAAATCAGAAGAAGTGGCAAAGAAACAACTTTCCAAAGACTCCATCTTATCT CTGTATGGCACAGGAACCATTCAACAGCAAAGTACTCCTGGTGTATTTATGGGACCCACAAATATACCAT TTACCTCACAAGCACCAGCTGCATTTCAGGGCTTTCCATCGATGGGCGTGCCTGTGCCTGCAGCTCCTGG CCTTATAGGAAATGTGATGGGACAGAGTCCAAGCATGATGGTGGGCATGCCCATGCCCAATGGGTTTATG GGAAATGCACAAACTGGTGTGATGCCACTTCCTCAGAACGTTGTTGGCCCCCAAGGAGGAATGGTGGGAC AAATGGGTGCACCCCAGAGTAAGTTTGGCCTGCCGCAAGCTCAGCAGCCCCAGTGGAGCCTCTCACAGAT AATGCAGAAGGGTGATGCTGTTCTCCAGCACTCCATCAGTGCAATCTACTGGCCAATGACAAGGTGGTTA AAATGTCCTTTAGTAGATGAATCAGCAGATGGCTGGCATGAGTATCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238225 representing NM_001281439 Red=Cloning site Green=Tags(s) MATRSCREKAQKLNEQHQLILSKLLREEDNKYCADCEAKGPRWASWNIGVFICIRCAGIHRNLGVHISRV KSVNLDQWTAEQIQCMQDMGNTKARLLYEANLPENFRRPQTDQAVEFFIRDKYEKKKYYDKNAIAITNKE KEKKKEEKKREKEPEKPAKPLTAEKLQKKDQQLEPKKSTSPKKAAEPTVDLLGLDGPAVAPVTNGNTTVP PLNDDLDIFGPMISNPLPATVMPPAQGTPSAPAAATLSTVTSGDLDLFTEQTTKSEEVAKKQLSKDSILS LYGTGTIQQQSTPGVFMGPTNIPFTSQAPAAFQGFPSMGVPVPAAPGLIGNVMGQSPSMMVGMPMPNGFM GNAQTGVMPLPQNVVGPQGGMVGQMGAPQSKFGLPQAQQPQWSLSQIMQKGDAVLQHSISAIYWPMTRWL KCPLVDESADGWHEYQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281439
ORF Size	1308 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281439.2
RefSeq Size	3356 bp
RefSeq ORF	1311 bp
Locus ID	60682
UniProt ID	Q8IYB5
Protein Pathways	Endocytosis
MW	48.3 kDa
Gene Summary	The protein encoded by this gene is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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