HARS2 (NM_001278732) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HARSL; HARSR; HisRS; HO3; PRLTS2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335622 representing NM_001278732. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTTGTGAGGGAGAAAATTCTTGATTTGGTTATCAGCTGCTTTAAACGTCATGGAGCAAAGGGGATG GACACCCCAGCATTTGAGCTGAAGGATTTTGACATTGCTGGTCAGTTTGACCCTATGATCCCCGATGCA GAGTGTTTGAAGATCATGTGTGAAATCCTAAGTGGATTGCAGTTGGGAGACTTTCTCATTAAGGTAAAT GACCGGCGGATTGTGGATGGGATGTTTGCTGTCTGTGGTGTTCCTGAAAGCAAGTTCCGTGCCATCTGC TCCTCCATAGATAAACTAGACAAGATGGCTTGGAAAGATGTGAGACATGAGATGGTGGTGAAGAAAGGC CTGGCTCCTGAGGTGGCTGATCGAATTGGGGACTATGTCCAGTGTCATGGTGGGGTATCCCTAGTAGAG CAAATGTTTCAGGATCCCAGACTATCCCAGAACAAGCAGGCCCTGGAGGGCCTGGGAGACCTAAAGCTG CTATTTGAATACCTGACTTTATTTGGAATTGCTGATAAGATCTCCTTTGACCTCAGCCTGGCTCGGGGC CTAGACTACTATACAGGAGTGATCTATGAAGCAGTGCTGCTGCAGACCCCAACTCAGGCTGGGGAGGAG CCCCTGAATGTGGGCAGTGTGGCTGCTGGTGGGCGCTATGATGGGCTGGTGGGCATGTTTGACCCCAAG GGCCACAAGGTGCCATGTGTGGGACTCAGCATTGGGGTTGAGCGAATCTTCTACATTGTGGAGCAGAGG ATGAAGACCAAAGGTGAGAAGGTGCGGACTACAGAGACTCAAGTGTTTGTGGCCACACCACAGAAGAAC TTTCTCCAAGAACGGTTGAAGCTTATTGCAGAGCTTTGGGATTCTGGAATCAAGGCAGAGATGCTATAC AAGAACAACCCCAAACTATTAACCCAGCTGCACTATTGTGAGAGCACAGGCATTCCACTGGTGGTCATT ATTGGTGAGCAAGAACTGAAAGAAGGGGTCATCAAGATCCGTTCAGTGGCCAGCAGAGAGGAGGTGGCC ATTAAACGGGAAAATTTTGTGGCTGAAATTCAGAAGCGACTGTCTGAGTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278732
Insert Size	1089 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278732.1
RefSeq Size	2393 bp
RefSeq ORF	1089 bp
Locus ID	23438
UniProt ID	P49590
Protein Pathways	Aminoacyl-tRNA biosynthesis
MW	40.5 kDa
Gene Summary	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) uses an alternate splice site and lacks two alternate in-frame exons in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. This isoform (3) lacks the transit peptide present in isoforms 1 and 2, hence it is not likely to localize to the mitochondrion.

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