RBKS (NM_001287580) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RBSK; RK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001287580, the custom clone sequence may differ by one or more nucleotides ATGACGTCCATGGTGTGTAAGGTTGGCAAAGATTCTTTTGGCAATGATTATATAGAAAACTTAAAACAGA ATGATATTTCTACAGAATTTACATATCAGACTAAAGATGCTGCTACAGGAACTGCTTCTATAATTGTCAA TAATGAAGGCCAGAATATCATTGTCATAGTGGCTGGAGCAAATTTACTTTTGAATACGGAGGATCTGAGG GCAGCAGCCAATGTCATTAGCAGAGCCAAAGTCATGGTCTGCCAGCTCGAAATAACTCCAGCAACTTCTT TGGAAGCCCTAACAATGGCCCGCAGGAGTGGAGTGAAAACCTTGTTCAATCCAGCCCCTGCCATTGCTGA CCTGGATCCCCAGTTCTACACCCTCTCAGATGTGTTCTGCTGCAATGAAAGTGAGGCTGAGATTTTAACT GGCCTCACGGTGGGCAGCGCTGCAGATGCTGGGGAGGCTGCATTAGTGCTCTTGAAAAGGGGCTGCCAGG TGGTAATCATTACCTTAGGGGCTGAAGGATGTGTGGTGCTGTCACAGACAGAACCTGAGCCAAAGCACAT TCCCACAGAGAAAGTCAAGGCTGTGGATACCACGGGTGCTGGTGACAGCTTTGTGGGAGCTCTGGCCTTC TACCTGGCTTACTATCCAAATCTGTCCTTGGAAGACATGCTCAACAGATCCAATTTCATTGCAGCAGTCA GTGTCCAGGCTGCAGGAACACAGTCATCTTACCCTTACAAAAAAGACCTTCCGCTTACTCTGTTTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001287580
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287580.1, NP_001274509.1
RefSeq Size	2395 bp
RefSeq ORF	768 bp
Locus ID	64080
UniProt ID	Q9H477
Protein Families	Druggable Genome
Protein Pathways	Pentose phosphate pathway
Gene Summary	This gene encodes a member of the carbohydrate kinase PfkB family. The encoded protein phosphorylates ribose to form ribose-5-phosphate in the presence of ATP and magnesium as a first step in ribose metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (2) differs in its 5' UTR, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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