PSD (NM_001270966) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EFA6; EFA6A; PSD1; TYL
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333016 representing NM_001270966. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCTCTCAAGTCACCTGTGCCCTTTCTACCTGGGACGAGCCCCTCGGCTGATGGGCCTGACTCTTTC AGTTGTGTGTTCGAAGCCATCCTGGAGTCACACCGGGCCAAAGGCACCTCCTATACCAGCCTCGCCTCG CTGGAGGCCTTGGCCTCACCTGGCCCAACCCAGAGCCCCTTCTTCACCTTTGAGCTGCCTCCCCAACCC CCTGCACCCCGGCCCGACCCACCAGCTCCCGCCCCACTTGCCCCTCTTGAACCGGATTCTGGTACCAGC TCTGCTGCTGATGGTCCTTGGACACAGAGAGGGGAGGAGGAGGAGGCAGAGGCCAGAGCCAAGCTGGCC CCAGGGAGGGAGCCCCCTAGTCCCTGCCACTCAGAGGACAGCCTTGGGCTGGGGGCAGCACCCCTTGGC AGCGAACCACCCCTGAGCCAGCTGGTGTCCGACTCAGACTCAGAGCTGGACAGCACAGAGCGGCTGGCC CTGGGAAGCACAGACACCTTGTCCAATGGGCAGAAAGCGGACCTGGAGGCTGCGCAGCGCCTGGCCAAG AGGCTGTACCGACTAGATGGCTTCAGGAAGGCCGATGTGGCCCGGCACCTGGGCAAGAACAATGACTTC AGCAAACTGGTGGCTGGGGAGTACCTCAAGTTCTTTGTCTTCACGGGCATGACTCTGGACCAAGCTCTC AGGGTGTTTCTGAAGGAGCTGGCCTTAATGGGTGAGACCCAGGAACGAGAGCGCGTGCTGGCCCACTTC TCCCAGCGATACTTCCAGTGCAATCCTGAAGCCCTGTCCTCAGAGGACGGCGCCCACACGCTGACCTGT GCGCTCATGCTGCTCAACACGGATCTCCACGGCCATAACATCGGGAAGCGCATGACCTGCGGGGACTTC ATCGGGAACCTGGAGGGCCTCAATGATGGCGGCGACTTCCCTAGGGAGCTGCTCAAGGCCTTGTACAGC TCCATCAAGAATGAGAAGCTGCAGTGGGCCATAGACGAGGAGGAGCTGAGACGCTCTCTGTCTGAGTTG GCCGACCCCAACCCCAAGGTCATCAAGCGGATCAGCGGGGGCAGTGGCAGTGGCTCCAGCCCTTTCCTG GACCTGACTCCCGAGCCTGGGGCTGCCGTCTACAAGCACGGGGCCCTGGTGCGAAAGGTGCACGCAGAC CCTGACTGCAGGAAGACACCTCGGGGCAAGCGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATG ATCCTCTACCTGCAGAAGGAGGAGTACAAGCCTGGGAAGGCCCTTTCAGAGACGGAGCTCAAGAATGCC ATCAGCATCCACCATGCCCTGGCCACTCGTGCCAGTGACTACAGCAAGAGGCCCCACGTCTTCTACCTG CGCACAGCTGACTGGCGGGTCTTCCTCTTCCAGGCCCCGAGCCTGGAGCAGATGCAGTCCTGGATCACT CGCATCAATGTAGTAGCCGCTATGTTCTCTGCGCCCCCCTTCCCAGCTGCTGTTAGCTCCCAAAAGAAG TTCAGCCGCCCTCTCCTGCCCAGCGCTGCCACCCGCCTCTCCCAGGAGGAGCAGGTGCGGACCCACGAG GCCAAGCTGAAGGCCATGGCAAGTGAGCTGCGGGAGCACCGGGCCGCCCAGCTGGGCAAGAAGGGCCGG GGCAAGGAGGCTGAAGAGCAGCGGCAGAAGGAGGCCTACCTGGAGTTTGAGAAATCCCGCTACAGCACC TATGCAGCGCTGCTTCGGGTCAAGCTGAAGGCAGGCAGTGAGGAGCTGGATGCAGTGGAGGCAGCACTG GCCCAGGCCGGGAGCACAGAGGATGGACTCCCTCCTTCTCACTCCAGTCCCTCCCTGCAGCCCAAACCC TCCAGCCAGCCCCGGGCTCAGCGTCACAGCTCAGAGCCTCGGCCAGGGGCAGGCAGTGGGCGGCGGAAG CCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270966
Insert Size	1938 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270966.1
RefSeq Size	3382 bp
RefSeq ORF	1938 bp
Locus ID	5662
UniProt ID	A5PKW4
Protein Pathways	Endocytosis
MW	70.4 kDa
Gene Summary	This gene encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

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