PSD (NM_001270966) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EFA6; EFA6A; PSD1; TYL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234648 representing NM_001270966 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTCAAGTCACCTGTGCCCTTTCTACCTGGGACGAGCCCCTCGGCTGATGGGCCTGACTCTTTCA GTTGTGTGTTCGAAGCCATCCTGGAGTCACACCGGGCCAAAGGCACCTCCTATACCAGCCTCGCCTCGCT GGAGGCCTTGGCCTCACCTGGCCCAACCCAGAGCCCCTTCTTCACCTTTGAGCTGCCTCCCCAACCCCCT GCACCCCGGCCCGACCCACCAGCTCCCGCCCCACTTGCCCCTCTTGAACCGGATTCTGGTACCAGCTCTG CTGCTGATGGTCCTTGGACACAGAGAGGGGAGGAGGAGGAGGCAGAGGCCAGAGCCAAGCTGGCCCCAGG GAGGGAGCCCCCTAGTCCCTGCCACTCAGAGGACAGCCTTGGGCTGGGGGCAGCACCCCTTGGCAGCGAA CCACCCCTGAGCCAGCTGGTGTCCGACTCAGACTCAGAGCTGGACAGCACAGAGCGGCTGGCCCTGGGAA GCACAGACACCTTGTCCAATGGGCAGAAAGCGGACCTGGAGGCTGCGCAGCGCCTGGCCAAGAGGCTGTA CCGACTAGATGGCTTCAGGAAGGCCGATGTGGCCCGGCACCTGGGCAAGAACAATGACTTCAGCAAACTG GTGGCTGGGGAGTACCTCAAGTTCTTTGTCTTCACGGGCATGACTCTGGACCAAGCTCTCAGGGTGTTTC TGAAGGAGCTGGCCTTAATGGGTGAGACCCAGGAACGAGAGCGCGTGCTGGCCCACTTCTCCCAGCGATA CTTCCAGTGCAATCCTGAAGCCCTGTCCTCAGAGGACGGCGCCCACACGCTGACCTGTGCGCTCATGCTG CTCAACACGGATCTCCACGGCCATAACATCGGGAAGCGCATGACCTGCGGGGACTTCATCGGGAACCTGG AGGGCCTCAATGATGGCGGCGACTTCCCTAGGGAGCTGCTCAAGGCCTTGTACAGCTCCATCAAGAATGA GAAGCTGCAGTGGGCCATAGACGAGGAGGAGCTGAGACGCTCTCTGTCTGAGTTGGCCGACCCCAACCCC AAGGTCATCAAGCGGATCAGCGGGGGCAGTGGCAGTGGCTCCAGCCCTTTCCTGGACCTGACTCCCGAGC CTGGGGCTGCCGTCTACAAGCACGGGGCCCTGGTGCGAAAGGTGCACGCAGACCCTGACTGCAGGAAGAC ACCTCGGGGCAAGCGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATGATCCTCTACCTGCAGAAG GAGGAGTACAAGCCTGGGAAGGCCCTTTCAGAGACGGAGCTCAAGAATGCCATCAGCATCCACCATGCCC TGGCCACTCGTGCCAGTGACTACAGCAAGAGGCCCCACGTCTTCTACCTGCGCACAGCTGACTGGCGGGT CTTCCTCTTCCAGGCCCCGAGCCTGGAGCAGATGCAGTCCTGGATCACTCGCATCAATGTAGTAGCCGCT ATGTTCTCTGCGCCCCCCTTCCCAGCTGCTGTTAGCTCCCAAAAGAAGTTCAGCCGCCCTCTCCTGCCCA GCGCTGCCACCCGCCTCTCCCAGGAGGAGCAGGTGCGGACCCACGAGGCCAAGCTGAAGGCCATGGCAAG TGAGCTGCGGGAGCACCGGGCCGCCCAGCTGGGCAAGAAGGGCCGGGGCAAGGAGGCTGAAGAGCAGCGG CAGAAGGAGGCCTACCTGGAGTTTGAGAAATCCCGCTACAGCACCTATGCAGCGCTGCTTCGGGTCAAGC TGAAGGCAGGCAGTGAGGAGCTGGATGCAGTGGAGGCAGCACTGGCCCAGGCCGGGAGCACAGAGGATGG ACTCCCTCCTTCTCACTCCAGTCCCTCCCTGCAGCCCAAACCCTCCAGCCAGCCCCGGGCTCAGCGTCAC AGCTCAGAGCCTCGGCCAGGGGCAGGCAGTGGGCGGCGGAAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234648 representing NM_001270966 Red=Cloning site Green=Tags(s) MPLKSPVPFLPGTSPSADGPDSFSCVFEAILESHRAKGTSYTSLASLEALASPGPTQSPFFTFELPPQPP APRPDPPAPAPLAPLEPDSGTSSAADGPWTQRGEEEEAEARAKLAPGREPPSPCHSEDSLGLGAAPLGSE PPLSQLVSDSDSELDSTERLALGSTDTLSNGQKADLEAAQRLAKRLYRLDGFRKADVARHLGKNNDFSKL VAGEYLKFFVFTGMTLDQALRVFLKELALMGETQERERVLAHFSQRYFQCNPEALSSEDGAHTLTCALML LNTDLHGHNIGKRMTCGDFIGNLEGLNDGGDFPRELLKALYSSIKNEKLQWAIDEEELRRSLSELADPNP KVIKRISGGSGSGSSPFLDLTPEPGAAVYKHGALVRKVHADPDCRKTPRGKRGWKSFHGILKGMILYLQK EEYKPGKALSETELKNAISIHHALATRASDYSKRPHVFYLRTADWRVFLFQAPSLEQMQSWITRINVVAA MFSAPPFPAAVSSQKKFSRPLLPSAATRLSQEEQVRTHEAKLKAMASELREHRAAQLGKKGRGKEAEEQR QKEAYLEFEKSRYSTYAALLRVKLKAGSEELDAVEAALAQAGSTEDGLPPSHSSPSLQPKPSSQPRAQRH SSEPRPGAGSGRRKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270966
ORF Size	1935 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270966.1, NP_001257895.1
RefSeq Size	3382 bp
RefSeq ORF	1938 bp
Locus ID	5662
UniProt ID	A5PKW4
Protein Pathways	Endocytosis
MW	70.8 kDa
Gene Summary	This gene encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

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