MGAT5B (NM_001199172) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GnT-IX; GnT-VB
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331258 representing NM_001199172. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATCACCGTCAACCCCGATGGGAAGATAATGGTCAGAAGATGCCTGGTCACCCTGAGACCCTTTCGG CTTTTTGTCCTGGGCATCGGCTTCTTCACTCTCTGCTTCCTGATGACGTCTCTGGGAGGCCAGTTCTCG GCCCGGCGCCTGGGGGACTCGCCATTCACCATCCGCACAGAAGTGATGGGGGGCCCCGAGTCCCGCGGC GTCCTGCGCAAGATGAGCGACCTGCTGGAGCTGATGGTGAAGCGCATGGACGCACTGGCCAGGCTGGAG AACAGCAGTGAGCTGCACCGGGCCGGCGGCGACCTGCACTTTCCCGCAGACAGGATGCCCCCTGGGGCC GGCCTCATGGAGCGGATCCAGGCTATTGCCCAGAACGTCTCCGACATCGCTGTGAAGGTGGACCAGATC CTGCGCCACAGTCTGCTCCTGCACAGCAAGGTGTCAGAAGGCCGGCGGGACCAGTGTGAGGCACCCAGT GACCCCAAGTTCCCTGACTGCTCAGGGAAGGTGGAGTGGATGCGTGCCCGCTGGACCTCTGACCCCTGC TACGCCTTCTTTGGGGTGGACGGCACCGAGTGCTCCTTCCTCATCTACCTCAGTGAGGTCGAGTGGTTC TGCCCCCCGCTGCCCTGGAGGAACCAGACGGCTGCCCAGAGGGCACCCAAGCCCCTCCCCAAAGTCCAG GCAGTTTTCCGAAGCAACCTGTCCCACCTTCTGGACCTGATGGGCAGCGGGAAGGAGTCCCTGATCTTC ATGAAGAAGCGGACCAAGAGGCTCACAGCCCAGTGGGCGCTGGCTGCCCAGCGCCTGGCACAGAAGCTG GGGGCCACCCAGAGGGACCAGAAGCAGATCCTGGTCCACATCGGCTTCCTGACGGAGGAGTCCGGGGAC GTGTTCAGCCCTCGGGTCCTGAAGGGCGGGCCCCTAGGGGAGATGGTGCAGTGGGCGGACATTCTGACT GCACTCTATGTCCTGGGCCATGGCCTGCGGGTCACAGTCTCCCTGAAGGAGCTGCAGAGTAACTTAGGG GTACCGCCAGGCCGGGGAAGCTGCCCGCTCACCATGCCCCTGCCCTTCGACCTCATCTACACCGACTAC CACGGCCTGCAGCAGATGAAGCGGCACATGGGACTCTCCTTCAAGAAGTACCGGTGCCGAATCAGGGTC ATCGACACCTTCGGGACGGAACCTGCGTACAACCACGAGGAGTACGCCACGCTGCACGGCTACCGGACC AACTGGGGCTACTGGAACCTCAACCCCAAGCAGTTCATGACCATGTTTCCTCATACCCCCGACAACTCC TTCATGGGCTTCGTGTCCGAGGAGCTCAACGAGACGGAGAAGCGGCTCATCAAAGGCGGCAAGGCCAGC AACATGGCCGTGGTGTACGGCAAGGAGGCGAGCATCTGGAAGCTCCAGGGGAAGGAGAAGTTCCTGGGC ATCCTGAACAAATACATGGAGATCCATGGCACCGTGTACTACGAGAGCCAGCGGCCCCCCGAGGTGCCA GCCTTTGTGAAGAACCACGGCCTCTTACCGCAGCCTGAGTTTCAGCAGCTGCTGCGCAAGGCCAAACTC TTCATCGGGTTTGGCTTCCCCTACGAGGGCCCCGCCCCCCTGGAGGCCATCGCCAATGGTTGCATCTTC CTGCAGTCCCGCTTCAGCCCGCCCCACAGCTCCCTCAACCACGAGTTCTTCCGAGGCAAGCCCACCTCC AGAGAGGTGTTCTCCCAGCATCCCTACGCGGAGAACTTCATCGGCAAGCCCCACGTGTGGACAGTCGAC TACAACAACTCAGAGGAGTTTGAAGCAGCCATCAAGGCCATTATGAGAACTCAGGTAGACCCCTACCTA CCCTACGAGTACACCTGCGAGGGGATGCTGGAGCGGATCCACGCCTACATCCAGCACCAGGACTTCTGC AGAGCTCCAGACCCTGCCCTACCAGAGGCCCACGCCCCGCAGAGCCCCTTTGTCCTGGCCCCCAATGCC ACCCACCTCGAGTGGGCTCGGAACACCAGCTTGGCTCCTGGGGCCTGGCCCCCCGCGCACGCCCTGCGG GCCTGGCTGGCCGTGCCTGGGAGGGCCTGCACCGACACCTGCCTGGACCACGGGCTAATCTGTGAGCCC TCCTTCTTCCCCTTCCTGAACAGCCAGGACGCCTTCCTCAAGCTGCAGGTGCCCTGTGACAGCACCGAG TCGGAGATGAACCACCTGTACCCGGCGTTCGCCCAGCCTGGCCAGGAGTGCTACCTGCAGAAGGAGCCT CTGCTCTTCAGCTGCGCCGGCTCCAACACCAAGTACCGCCGGCTCTGCCCCTGCCGCGACTTCCGCAAG GGCCAGGTGGCCTTGTGCCAGGGCTGTCTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001199172
Insert Size	2379 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199172.1
RefSeq Size	4252 bp
RefSeq ORF	2379 bp
Locus ID	146664
UniProt ID	Q3V5L5
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
MW	89.5 kDa
Gene Summary	The MGAT5B gene encodes a beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.155) that functions in the synthesis of complex cell surface N-glycans (Kaneko et al., 2003 [PubMed 14623122]).[supplied by OMIM, Nov 2008] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and contains an alternate in-frame exon compared to variant 2. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an internal 2 aa segment compared to isoform 2.

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