MGAT5B (NM_001199172) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GnT-IX; GnT-VB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234885 representing NM_001199172 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCACCGTCAACCCCGATGGGAAGATAATGGTCAGAAGATGCCTGGTCACCCTGAGACCCTTTCGGC TTTTTGTCCTGGGCATCGGCTTCTTCACTCTCTGCTTCCTGATGACGTCTCTGGGAGGCCAGTTCTCGGC CCGGCGCCTGGGGGACTCGCCATTCACCATCCGCACAGAAGTGATGGGGGGCCCCGAGTCCCGCGGCGTC CTGCGCAAGATGAGCGACCTGCTGGAGCTGATGGTGAAGCGCATGGACGCACTGGCCAGGCTGGAGAACA GCAGTGAGCTGCACCGGGCCGGCGGCGACCTGCACTTTCCCGCAGACAGGATGCCCCCTGGGGCCGGCCT CATGGAGCGGATCCAGGCTATTGCCCAGAACGTCTCCGACATCGCTGTGAAGGTGGACCAGATCCTGCGC CACAGTCTGCTCCTGCACAGCAAGGTGTCAGAAGGCCGGCGGGACCAGTGTGAGGCACCCAGTGACCCCA AGTTCCCTGACTGCTCAGGGAAGGTGGAGTGGATGCGTGCCCGCTGGACCTCTGACCCCTGCTACGCCTT CTTTGGGGTGGACGGCACCGAGTGCTCCTTCCTCATCTACCTCAGTGAGGTCGAGTGGTTCTGCCCCCCG CTGCCCTGGAGGAACCAGACGGCTGCCCAGAGGGCACCCAAGCCCCTCCCCAAAGTCCAGGCAGTTTTCC GAAGCAACCTGTCCCACCTTCTGGACCTGATGGGCAGCGGGAAGGAGTCCCTGATCTTCATGAAGAAGCG GACCAAGAGGCTCACAGCCCAGTGGGCGCTGGCTGCCCAGCGCCTGGCACAGAAGCTGGGGGCCACCCAG AGGGACCAGAAGCAGATCCTGGTCCACATCGGCTTCCTGACGGAGGAGTCCGGGGACGTGTTCAGCCCTC GGGTCCTGAAGGGCGGGCCCCTAGGGGAGATGGTGCAGTGGGCGGACATTCTGACTGCACTCTATGTCCT GGGCCATGGCCTGCGGGTCACAGTCTCCCTGAAGGAGCTGCAGAGTAACTTAGGGGTACCGCCAGGCCGG GGAAGCTGCCCGCTCACCATGCCCCTGCCCTTCGACCTCATCTACACCGACTACCACGGCCTGCAGCAGA TGAAGCGGCACATGGGACTCTCCTTCAAGAAGTACCGGTGCCGAATCAGGGTCATCGACACCTTCGGGAC GGAACCTGCGTACAACCACGAGGAGTACGCCACGCTGCACGGCTACCGGACCAACTGGGGCTACTGGAAC CTCAACCCCAAGCAGTTCATGACCATGTTTCCTCATACCCCCGACAACTCCTTCATGGGCTTCGTGTCCG AGGAGCTCAACGAGACGGAGAAGCGGCTCATCAAAGGCGGCAAGGCCAGCAACATGGCCGTGGTGTACGG CAAGGAGGCGAGCATCTGGAAGCTCCAGGGGAAGGAGAAGTTCCTGGGCATCCTGAACAAATACATGGAG ATCCATGGCACCGTGTACTACGAGAGCCAGCGGCCCCCCGAGGTGCCAGCCTTTGTGAAGAACCACGGCC TCTTACCGCAGCCTGAGTTTCAGCAGCTGCTGCGCAAGGCCAAACTCTTCATCGGGTTTGGCTTCCCCTA CGAGGGCCCCGCCCCCCTGGAGGCCATCGCCAATGGTTGCATCTTCCTGCAGTCCCGCTTCAGCCCGCCC CACAGCTCCCTCAACCACGAGTTCTTCCGAGGCAAGCCCACCTCCAGAGAGGTGTTCTCCCAGCATCCCT ACGCGGAGAACTTCATCGGCAAGCCCCACGTGTGGACAGTCGACTACAACAACTCAGAGGAGTTTGAAGC AGCCATCAAGGCCATTATGAGAACTCAGGTAGACCCCTACCTACCCTACGAGTACACCTGCGAGGGGATG CTGGAGCGGATCCACGCCTACATCCAGCACCAGGACTTCTGCAGAGCTCCAGACCCTGCCCTACCAGAGG CCCACGCCCCGCAGAGCCCCTTTGTCCTGGCCCCCAATGCCACCCACCTCGAGTGGGCTCGGAACACCAG CTTGGCTCCTGGGGCCTGGCCCCCCGCGCACGCCCTGCGGGCCTGGCTGGCCGTGCCTGGGAGGGCCTGC ACCGACACCTGCCTGGACCACGGGCTAATCTGTGAGCCCTCCTTCTTCCCCTTCCTGAACAGCCAGGACG CCTTCCTCAAGCTGCAGGTGCCCTGTGACAGCACCGAGTCGGAGATGAACCACCTGTACCCGGCGTTCGC CCAGCCTGGCCAGGAGTGCTACCTGCAGAAGGAGCCTCTGCTCTTCAGCTGCGCCGGCTCCAACACCAAG TACCGCCGGCTCTGCCCCTGCCGCGACTTCCGCAAGGGCCAGGTGGCCTTGTGCCAGGGCTGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234885 representing NM_001199172 Red=Cloning site Green=Tags(s) MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGV LRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILR HSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPP LPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQ RDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQSNLGVPPGR GSCPLTMPLPFDLIYTDYHGLQQMKRHMGLSFKKYRCRIRVIDTFGTEPAYNHEEYATLHGYRTNWGYWN LNPKQFMTMFPHTPDNSFMGFVSEELNETEKRLIKGGKASNMAVVYGKEASIWKLQGKEKFLGILNKYME IHGTVYYESQRPPEVPAFVKNHGLLPQPEFQQLLRKAKLFIGFGFPYEGPAPLEAIANGCIFLQSRFSPP HSSLNHEFFRGKPTSREVFSQHPYAENFIGKPHVWTVDYNNSEEFEAAIKAIMRTQVDPYLPYEYTCEGM LERIHAYIQHQDFCRAPDPALPEAHAPQSPFVLAPNATHLEWARNTSLAPGAWPPAHALRAWLAVPGRAC TDTCLDHGLICEPSFFPFLNSQDAFLKLQVPCDSTESEMNHLYPAFAQPGQECYLQKEPLLFSCAGSNTK YRRLCPCRDFRKGQVALCQGCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199172
ORF Size	2376 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199172.2
RefSeq Size	4252 bp
RefSeq ORF	2379 bp
Locus ID	146664
UniProt ID	Q3V5L5
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
MW	90 kDa
Gene Summary	The MGAT5B gene encodes a beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.155) that functions in the synthesis of complex cell surface N-glycans (Kaneko et al., 2003 [PubMed 14623122]).[supplied by OMIM, Nov 2008]

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