TNFAIP3 (NM_001270507) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A20; AISBL; OTUD7C; TNFA1P2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330856 representing NM_001270507. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAATATGCGGAAAGCTGTGAAGATACGGGAG AGAACTCCAGAAGACATTTTTAAACCTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATAC ACACTGGAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCATCCACAAAGCCCTCATC GACAGAAACATCCAGGCCACCCTGGAAAGCCAGAAGAAACTCAACTGGTGTCGAGAAGTCCGGAAGCTT GTGGCGCTGAAAACGAACGGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGGCGTT CAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCAAGGAAACAGACACACGCAACTTT AAATTCCGCTGGCAACTGGAGTCTCTCAAATCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACT CGGAACTGGAATGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCATGGCCCGAAGT GGACTTCAGTACAACTCACTGGAAGAAATACACATATTTGTCCTTTGCAACATCCTCAGAAGGCCAATC ATTGTCATTTCAGACAAAATGCTAAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGT GGAATTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCCATTGTTCTCGGCTATGAC AGCCATCATTTTGTACCCTTGGTGACCCTGAAGGACAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTT AACAGAGACCGGGGAAGATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAGATGAAG GAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGTCCAAGGCTGGGACCATGGCACAACT CATCTCATCAATGCCGCAAAGTTGGATGAAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTAC TTTGAACTTGTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAGGAGAGAGGGGCAC GCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTTCTCTCATGGATGTAAAATGTGAAACGCCCAAC TGCCCCTTCTTCATGTCTGTGAACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAAT CAAAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCCCTGGCATGGCGCTCGGGGCC TCTCGGGGAGAAGCCTATGAGCCCTTGGCGTGGAACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCC CCACCGACAGCACCCAGCCCTTTTCTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCCCCGGCTGC CCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGTTGCCACAACGCCCGGCAACTTCACGCC AGCCACGCCCCAGACCACACAAGGCACTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACC AGGACATTTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCCTCCTCCAGCCTCAGC ACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAAGTCAGATCCCTCGCGGCTCGTCCGGAGCCCCTCC CCGCATTCTTGCCACAGAGCTGGAAACGACGCCCCTGCTGGCTGCCTGTCTCAAGCTGCACGGACTCCT GGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTATTTTGGGACTCCAGAAAACAAG GGCTTTTGCACACTGTGTTTCATCGAGTACAGAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTC AGTCCCACAGCGTCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCACCCTTGGAAGC ACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAGCTCAGAATCAGAGATTTCATGAGGCCAAA AGGACAGAAGAGCAACTGAGATCGAGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGG CCCAAGTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGGAGCTCTGCATGGAGTGT CAGCATCCCAACCAGAGGATGGGCCCTGGGGCCCACCGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAG CAGCGTTGCCGGGCCCCCGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACGAATGC TTTCAGTTCAAGCAGATGTATGGCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270507
Insert Size	2373 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270507.1
RefSeq Size	4729 bp
RefSeq ORF	2373 bp
Locus ID	7128
UniProt ID	P21580
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway
MW	89.6 kDa
Gene Summary	This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All three variants encode the same protein.

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