TNFAIP3 Mouse Monoclonal Antibody [Clone ID: 59A426]

Specifications

Product Data
Clone Name	59A426
Applications	CyTOF-ready, FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Flow Cytometry: 2.5 ug/ml, Immunohistochemistry-Paraffin: 5 - 10 ug/ml, Flow (Intracellular): 2.5 ug/ml, Western Blot: 2-4 ug/ml, Immunoprecipitation: 1-2 ug/ml, Immunocytochemistry/ Immunofluorescence: 10 ug/ml, Immunohistochemistry: 5 - 10 ug/ml, Simple Western: 1:100, CyTOF-ready
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length recombinant human A20. The epitope has been mapped to the C-terminal portion of A20 (amino acids 440-790)
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	TNF alpha induced protein 3
Database Link	NP_006281 Entrez Gene 21929 MouseEntrez Gene 683206 RatEntrez Gene 7128 Human UniProt ID P21580
Background	A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase (Evans, 2005). The A20 monoclonal antibody (clone 59A426) has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology.
Synonyms	A20; OTUD7C; TNFA1P2
Note	The A20 antibody (clone 59A426) has also been used in Immunohistochemistry-Paraffin (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway

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