AAK1 (BC002695) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	adaptor-associated kinase 1; AP2 associated kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC002695, the custom clone sequence may differ by one or more nucleotides ATGAAGAAGTTTTTCGACTCCCGGCGAGAGCAGGGCGGCTCTGGCCTGGGCTCCGGCTCCAGCGGAGGAG GGGGCAGCACCTCGGGCCTGGGCAGTGGCTACATCGGAAGAGTCTTCGGCATCGGGCGACAGCAGGTCAC AGTGGACGAGGTGTTGGCGGAAGGTGGATTTGCTATTGTATTTCTGGTGAGGACAAGCAATGGGATGAAA TGTGCCTTGAAACGCATGTTTGTCAACAATGAGCATGATCTCCAGGTGTGCAAGAGAGAAATCCAGATAA TGAGGGATCTTTCAGGGCACAAGAATATTGTGGGTTACATTGATTCTAGTATCAACAACGTGAGTAGCGG TGATGTATGGGAAGTGCTCATTCTGATGGACTTTTGTAGAGGTGGCCAGGTGGTAAACCTGATGAACCAG CGCCTGCAAACAGGCTTTACAGAGAATGAAGTGCTCCAGATATTTTGTGATACCTGTGAAGCTGTTGCCC GCCTGCATCAGTGCAAAACTCCTATTATCCACCGGGACCTGAAGGTTGAAAACATCCTCTTGCATGACCG AGGCCACTATGTCCTGTGTGACTTTGGAAGCGCCACCAACAAATTCCAGAATCCACAAACTGAGGGAGTC AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTGTCCTATCGAGCACCAGAAATGGTCAACCTGT ACAGTGGCAAAATCATCACTACGAAGGCAGACATTTGGGCTCTTGGATGTTTGTTGTATAAATTATGCTA CTTCACTTTGCCATTTGGGGAAAGTCAGGTGGCAATTTGTGATGGAAACTTCACAATTCCTGATAATTCT CGATATTCTCAAGACATGCACTGCCTAATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA TTTACCAGGTGTCCTACTTCTCATTTAAGCTACTCAAGAAAGAGTGCCCAATTCCAAATGTACAGAACTC TCCCATTCCTGCAAAGCTTCCTGAACCAGTGAAAGCCAGTGAGGCAGCTGCAAAAAAGACCCAGCCAAAG GCCAGACTGACAGATCCCATTCCCACCACAGAGACTTCAATTGCACCCCGCCAGAGGCCTAAAGCTGGGC AGACTCAGCCGAACCCAGGAATCCTTCCCATCCAGCCAGCGCTGACACCCCGGAAGAGGGCCACTGTTCA GCCCCCACCTCAGGCTGCAGGATCCAGCAATCAGCCTGGCCTTTTAGCCAGTGTTCCCCAACCAAAACCC CAAGCCCCACCCAGCCAGCCTCTGCCGCAAACTCAGGCCAAGCAGCCACAGGCTCCTCCCACTCCACAGC AGACGCCTTCTACTCAGGCCCAGGGTCTGCCCGCTCAGGCCCAGGCCACACCCCAGCACCAGCAGCATAC AATAAAACTTAGTATGAAACTTTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for mutant BC002695 unedited CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC CGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAAACCATCGGTATTT TGCTTTGCTGCTCCCTATTCGCAAGATGAAGAAGCTTTTCGACTCCCGGCGAGAGCAGGGCGGCTCTGGC CTGGGCTCCGGCTCCAGCGGAGGAGGGGGCAGCACCTCGGGCCTGGGCAGTGGCTACATCGGAAGAGTCT TCGGCATCGGGCGACAGCAGGGTCACAGTGGACGAGGGTGTTGGCGGGAAGTGGATTTGCTATTTGTATT TTCTGGGTGAGGGACAAGCCAATGGGATGAAAATGTGGCCTTGATAGCCCGCTAGTGTTTGTCCAACAAT GGAGCACATGGATTCTCCCAGTGTGTGCAAAGAGAGAGAAATCTCACGATATATGAGAGGATCCCTTTCA GGGGCACCAAGAAATTTGGGTGGGTACACATGAGATCTCTAGTAAAAAACACCTTGAATGGCGGTGTATT ATAGGGAGAATGTGCTTATTCGTTATGGCCTTTTGTAGGGGGTGGCCAGGTGGTAACCCTGATGAACCCC CGCCCCTGGAAAACGCGCTTTCCAAAAAAAGGAAGGGGCCCAAAATTTTTGGAATCCGTGAAAACCTTTT CCCCCCCCTCTCAGGCAAATCCTCTTATTATCCCCGACCCGCAGGTGGTAAAAATCCTTCTTGTTGAAAA AGGAGCGCCAAAGCCCGGGGGCAACGGAGAACCCCCCCCCAAATTCTCAAAACCCCACCTAGGGAGGCAT TGCGGTCTAGAAAGAGAAATAAAGAACAACGCGTGCTCTCTATCGCACACAAAGTGTGCCCTCTGTGTGG CGATATTCTCTCAAGCGAGACATTTGCCGCTGAGATGTGTGTTGAATATACCGAGCACCTGCGCAATCGG AACCATGGCGCAGTGGTTGGGAGCTACACTCTGCAGATATACATACTGAAGCACGTCGCGCTCTATGATG T Kinase domain sequence (showhide) >SC323545 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation CRKCTRAGTGTGGCGGAGGTGGATTTGCTATTGTATTTCTGGTGAGGACAAGCAATGGGATGAAATGTGC CTTGATGCGCATGTTTGTCAACAATGAGCATGATCTCCAGGTGTGCAAGAGAGAAATCCAGATAATGAGG GATCTTTCAGGGCACAAGAATATTGTGGGTTACATTGATTCTAGTATCAACAACGTGAGTAGCGGTGATG TATGGGAAGTGCTCATTCTGATGGACTTTTGTAGAGGTGGCCAGG
Restriction Sites	Please inquire
ACCN	BC002695
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC002695.2, AAH02695.1
RefSeq Size	1513 bp
RefSeq ORF	1425 bp
Locus ID	22848
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a member of the SNF1 subfamily of serine/threonine protein kinases. Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. The encoded protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. This kinase has been shown to play an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of this kinase are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Aug 2020]

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