MRRF (NM_199177) Human Untagged Clone

CAT#: SC322027

MRRF (untagged)-Human mitochondrial ribosome recycling factor (MRRF), nuclear gene encoding mitochondrial protein, transcript variant 2



  "NM_199177" in other vectors (4)

CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
MRRF mouse monoclonal antibody, clone OTI1D5 (formerly 1D5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms MRFF; MTRRF; RRF
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for SC322027 GATGTTTCCAAGGATTGTCTTCAGTCATGGCCTTGGGATTAAAGTGCTTCCGCATGGTCC
ACCCTACCTTTCGCAATTATCTTGCAGCCTCTATCAGACCCGTTTCAGAAGTTACACTGA
AGACAGTGCATGAAAGACAACATGGCCATAGGCAATACATGGCCTATTCAGCTGTACCAG
TCCGCCATTTTGCTACCAAGAAAGCCAAAGCCAAAGGGAAAGGACAGTCCCAAACCAGAG
TGAATATTAATGCTGCCTTGGTTGAGGATATAATCAACTTGGAAGAGGTGAATGAAGAAA
TGAAGTCTGTGATAGAAGCTCTCAAGGATAATTTCAATAAGACTCTCAATATAAGGACCT
CACCAGGATCCCTTGACAAGATTGCTGTGGTAACTGCTGACGGGAAGCTTGCTTTAAACC
AGATTAGCCAGATCTCCATGAAGTCGCCACAGCTGATTTTGGTGAATATGGCCAGCTTCC
CAGAGTGTACAGCTGCAGCTATCAAGGCTATAAGAGAAAGTGGAATGAATCTGAACCCAG
AAGTGGAAGGGACGCTAATTCGGGTACCCATTCCCCAATCAGCCAAATGGCCGATGACAC
AGTGGCAGAACTGGACAGGCATCTGGCAGTGAAGACCAAAGAACTCCTTGGATGAAAGTC
CACTGGGGCCAGCAATACTCCAGAGCCCAGTTTCTGCTGGATCCCATGGGTGGCACATTG
GGACTTCTCTCCCTCCCCCATCTACACAGAAGACTGTCACCATGCTGACAGAAGCCTGTC
CTTGTAAGGCCCAGCCTTCCAGGGGAACACTCAGACATGTTCATTCTCTTCCTGCTTCTG
CTCTGGGCCGGTGGGTGGCTCTCAGAAAATACTTGCTGCTGGCAAAAGGCCTGTACTCAG
GCATTTGCTTTGACTTGATGTTGCCAAGGGACTGAGGCCATTGGCAGGCTTAGTACCACC
TGCTCCTCATCTTAGGAGTCTCCTTTTCAAATAATTAGGCTCTGTTCCCATTTTAAAACT
CTGATATTGGCCTTCACCTGTGACTGGACACTTTACTAGAGGCCCATTTTCACTAAACAA
TAAAATCTAAATAAATTGGAAGGAATAACAACCACAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_199177
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_199177.1, NP_954646.1
RefSeq Size 1627 bp
RefSeq ORF 606 bp
Locus ID 92399
UniProt ID Q96E11
Protein Families Transmembrane
Gene Summary This gene encodes a ribosome recycling factor, which is a component of the mitochondrial translational machinery. The encoded protein, along with mitochondrial elongation factor 2, functions in ribosomal recycling at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Oct 2016]
Transcript Variant: This variant (2) lacks an exon in the 3' coding region resulting in a frameshift, compared to isoform 1. The sequence of the 5' UTR of this variant has not been determined. The resulting isoform (2) has a shorter and distinct C-terminu
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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