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CYP7B1 (NM_004820) Human Untagged Clone
CAT＃: SC316629
CYP7B1 (untagged)-Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1)
CNY 3,776.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 3,340.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CBAS3; CP7B; SPG5A |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC316629 sequence for NM_004820 edited (data generated by NextGen Sequencing)
ATGGCAGGAGAAGTGTCCGCGGCCACGGGCCGCTTTTCGCTGGAGCGGTTGGGCCTCCCG GGCCTGGCCCTCGCCGCGGCCCTGCTGCTCCTGGCCCTCTGCTTGCTTGTCCGGCGCACC AGGAGACCCGGTGAGCCTCCATTGATAAAAGGTTGGCTTCCTTATCTTGGAGTGGTCCTG AACTTACGAAAAGACCCCTTAAGGTTCATGAAAACACTTCAAAAGCAACATGGTGACACT TTCACAGTTCTTCTTGGTGGAAAGTACATAACATTTATCCTGGACCCCTTCCAGTACCAG CTAGTGATAAAAAATCATAAACAATTAAGCTTTCGAGTATTTTCTAATAAATTATTAGAG AAAGCATTTAGCATCAGTCAGTTGCAAAAAAATCATGACATGAATGATGAGCTTCACCTC TGCTATCAATTTTTGCAAGGCAAATCTTTGGACATACTCTTGGAAAGCATGATGCAGAAT CTAAAACAAGTTTTTGAACCCCAGCTGTTAAAAACCACAAGTTGGGACACGGCAGAACTG TATCCATTCTGCAGCTCAATAATATTTGAGATCACATTTACAACTATATATGGAAAAGTT ATTGTTTGTGACAACAACAAATTTATTAGTGAGCTAAGAGATGATTTTTTAAAATTTGAT GACAAGTTTGCATATTTAGTATCCAACATACCCATTGAGCTTCTAGGAAATGTCAAGTCT ATTAGAGAGAAAATTATAAAATGCTTCTCATCAGAAAAGTTAGCCAAGATGCAAGGATGG TCAGAAGTTTTTCAAAGCAGGCAAGATGTCCTGGAGAAATATTATGTGCACGAGGACCTT GAAATAGGAGCACATCATTTAGGCTTTCTCTGGGCCTCTGTGGCAAACACTATTCCAACT ATGTTCTGGGCAATGTATTATCTTCTGCGGCACCCAGAAGCTATGGCAGCAGTGCGTGAC GAAATTGACCGTTTGCTGCAGTCAACAGGTCAAAAGAAAGGGTCTGGATTTCCCATCCAC CTCACCAGAGAACAATTGGACAGCCTAATCTGCCTAGAAAGCAGCATTTTTGAAGCTTTA CGACTGTCCTCATATTCAACCACCATTCGTTTTGTTGAGGAGGATTTGACTCTCAGTTCA GAGACCGGGGACTACTGTGTGCGAAAGGGAGACTTGGTAGCCATCTTTCCTCCAGTCCTA CATGGTGACCCTGAAATCTTTGAAGCTCCAGAGGAGTTTAGATATGATCGTTTTATAGAA GATGGTAAGAAGAAAACCACCTTTTTCAAAAGAGGGAAAAAGCTGAAGTGTTACCTAATG CCGTTTGGAACTGGAACCAGCAAATGTCCAGGCCGATTTTTTGCACTTATGGAAATAAAA CAATTGTTGGTTATACTTTTAACTTATTTTGATTTAGAAATAATTGATGATAAGCCCATA GGACTAAACTACAGCCGCTTGTTGTTTGGTATTCAGTATCCAGATTCTGATGTTTTATTT AGATACAAAGTGAAATCTTAG Clone variation with respect to NM_004820.3 >OriGene 5' read for NM_004820 unedited
TGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCACGGTGACATTCCTGTC TGCGCTGCGGGCAGGCCAGCCTCTCCGGCCCTGGACTAGGGGGCGCCGCCGAGAGAAGCC GCCGCAGGCTGCAGAGTCGCCGACCGCGCTGTTCGCAGGCAGACCCCGGGCTGCCCACCG CGGCCTAGCGCGCGCCGGATGGCAGGAGAAGTGTCCGCGGCCACGGGCCGCTTTTCGCTG GAGCGGTTGGGCCTCCCGGGCCTGGCCCTCGCCGCGGCCCTGCTGCTCCTGGCCCTCTGC TTGCTTGTCCGGCGCACCAGGAGACCCGGTGAGCCTCCATTGATAAAAGGTTGGCTTCCT TATCTTGGGAGTGGTCCTGAACTTACGAAAAGACCCCTTAAGGTTCATGAAAACACTTCA AAAGCAACATGGTGACACTTTCACAGTTCTTCTTGGTGGAAAGTACATAACATTTATCCT GGACCCCTTCCAGTACCAGCTAGTGATAAAAAATCATAAACAATTAAGCTTTCGAGTATT TTCTAATAAATTATTAGAGAAAGCATTTAGCATCAGTCAGTTGCAAAAAAATCATGACAT GAATGATGAGCTTCACCTCTGCTATCAATTTTTGCAAGGCAAATCTTTGGACATACTCTT GGAAAGCATGATGCAAAATCTAAAACAAGTTTTTGAACCCCAGCTGTTAAAAACCACAAG TTGGGACACGGCAGAACTGTATCCATTCTGCAGCTCATATATTTNTGAGACACATTTACA CTATTTGGGGAAAGGTTTTTGGTTTGTGACACAACAAATTTATTAGTGAGCTAAGAGAAG GATTTTTTAAAATTTGAGGACCAGTTTGGCTTATTTAGTATCAACATACCCTTGG >Forward primer walk for NM_004820 unedited
CAAAGTCCAGCCGATTTTTGCACTTAGGAATAAAACAATTGTTGGTTATACTTTTAACTT ATTTTGATTTAGAAATAATTGATGATAAGCCCATAGGACTAAACTACAGCCGCTTGTTGT TTGGTATTCAGTATCCAGATTCTGATGTTTTATTTAGCTACAAAGTGAAATCTTAGAGAA GCTAAAAGGAAAGAAAATAAATCTATCAAAATTACCCTAAACATCCTAAGCTCATCTATT TCTTTTTAATTTCTGCAAATGTAATTGATTTATTTGTTTAAAAAGCGCTAATTTCTATTT GATCTGATATCAGTCCAGTTTGTCCTTAGTCACAAAGCCCATCATAATATAAAACAGGAT GGTGGCAGGAAAATGGACATCAAAATCAACTTAAGGGTAGGCTCAAAACAGGGTTGTTGT TGTTTTTTTAGTTTCTCCTTGTTGTGATTTTCACCTGTTATAATAAATGTACCTTCACAC CAATAGATTGGGCACTGTGGAATTTAAATCACTCAAATTGTTCTCTAATATGTAAAATTA CACTTTGAACTACTAGAAAATTGTGCTGGAAATGGACACAGTCTAACAAATTCCAAATTC TCACAGAAGAAGGAAATTGAATTTCCATGAGTTACACTGGATGAAAATGTTCCCTTCAAG TATATATTAATAATACCTATATTTCCAGGGTATGTGTGCATTAAATGAGTNTCACAGTAG ATTGAATGCTTCAACTTCACTTNCATATTAAATATGCATAAATGNTNCTTAATACTTTGN ATACTATGGCTCATAGAACAAATTNNCTGNNTACATAGACTTNCATGTTCAGNTAGATAC TGATAGATTAATGTTAGAACACAGAACTATATTATCAGAAATGCCTANATANAATAAGNN TATATGGGAGCAATGTGATAATACGCATGATATTAATTGNCTTAATAACNTAAGGTTGCA AATTGCATAACTTTCTAAGCTATTATA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004820 |
| Insert Size | 4600 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004820.2, NP_004811.1 |
| RefSeq Size | 2230 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 9420 |
| UniProt ID | O75881 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Primary bile acid biosynthesis |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222647 | CYP7B1 (Myc-DDK-tagged)-Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) |
CNY 3,768.00 |
|
| RC222647L1 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222647L2 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mGFP tagged |
CNY 5,890.00 |
|
| RC222647L3 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222647L4 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mGFP tagged |
CNY 5,890.00 |
|
| RG222647 | CYP7B1 (tGFP-tagged) - Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) |
CNY 4,370.00 |
