United States
Germany
Japan
United Kingdom
China
CYP7B1 (NM_004820) Human Tagged Lenti ORF Clone
CAT＃: RC222647L4
- LentiORF®
Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mGFP tagged
"NM_004820" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,890.00
Product images
CNY 1,999.00
CNY 3,340.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | CBAS3; CP7B; SPG5A |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC222647).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004820 |
| ORF Size | 1518 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004820.2 |
| RefSeq Size | 2395 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 9420 |
| UniProt ID | O75881 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Primary bile acid biosynthesis |
| MW | 58.3 kDa |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222647 | CYP7B1 (Myc-DDK-tagged)-Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) |
CNY 3,768.00 |
|
| RC222647L1 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222647L2 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mGFP tagged |
CNY 5,890.00 |
|
| RC222647L3 | Lenti ORF clone of Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG222647 | CYP7B1 (tGFP-tagged) - Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) |
CNY 4,370.00 |
|
| SC316629 | CYP7B1 (untagged)-Human cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1) |
CNY 3,776.00 |
