TRPT1 (AK026364) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026364, the custom clone sequence may differ by one or more nucleotides TTTTTTTTTTCATTGGCGGGTCCGCCGCGGTCGAGGATCCTGGAGGTTTGTCCCCGCCGCCCCGACGCTT CCGCCCGGTCCGTCGGCACGTCGGCCCTGCCTCCCGGACCACCCTGCGGGGCGCACCAGCGATCTGGGGT GCGGGGCTCGGCCTCCCTGCGCTCCTGGCTGACGGTGTGACCTTGGGTCTTAACCATGAACTTCTCTGGA GGAGGGAGGCAGGAAGCAGCAGGGTCCAGGGGTAGAAGGGCTCCCAGACCCCGAGAACAGGACCGAGACG TGCAGCTGTCCAAGGCTCTGTCCTATGCCCTGCGCCATGGGGCCTTGAAGCTGGGGCTTCCCATGGGAGC TGGTAAGTAGGGGCCTTGGAGGCTGGGGCTTGAGCCCAGAGAGAAACGTGAGCCTGTCTGCGACTCCTCC CTGATGGCTGCCTCCACCGCTCCCTGAGGAAGGCACAGAGTGCACTTCCTCCCCATGCACAGAGGAGAGC AAGGCCTAGAGCCCCTGTCATGTCCACAGCAGACTGAGGAGAGTTCTTGTCTGGGCCAGGCCTGGTGACC GCCCCCTCCAGCCAAGCAGCCAGATCAGCACTTACCCCGAGGGCGAGCCCTGGAGGGTACGGTGCAGCTG GTGGTGTGAGTGTGCACTCCGGGCCAGCGCTATGCTGGGCACTCACCTCCCTGCCCCTCCGTGAGGCAGA TCCTCTTTTTATGTAAGTTGAAAAAACTGCGGCCCAGAAAGGGGAGACTTTCTAAAGGTCCCACAGAGGA TGCCCAAGCGATCTCCAAGCCCAGACTTTGTTTCGGTTGCCTGTGGCTCCCTGTTAACCCTGGCCCAGTC CAGAGCAAGGTTAGCTCCTGAGCACCCTGCCTGCCCACAGATGGCTTCGTGCCCCTGGGCACCCTCCTGC AGTTGCCCCAGTTCCGCGGCTTCTCTGCTGAAGATGTGCAGCGCGTGGTGGACACCAATAGGAAGCAGCG GTTCGCCCTGCAGCTGGGGGATCCCAGCACTGGCCTTCTCATCCGGGCCAACCAGGGCCATTCCCTGCAG GTACCTAAGTTGGAGCTGATGCCCCTGGAGACACCGCAGGCCCTGCCCCCGATGCTAGTCCATGGTACAT TCTGGAAGCACTGGCCATCCATCCTACTCAAAGGCCTGTCCTGCCAGGGAAGGACGCACATTCACCTGGC CCCAGGACTGCCTGGAGACCCCGGTATCATCAGTGGTCAGTGCCCTCCCCTCCACCTAGCTACTCCCACC CACTCTGTCCTCCCAGGTCCCCTTCAAAGGTTACAACACTTGTCTGGGGCCACCCCATGTCCGGCATGGA GCAGCACCCCTGCATCCCCCAGCCCAAGACCCCAGTCTGCCTAGGTTGCTGACTGCACTCCAGGTGCCCA GGCCTCAGCTCCAGCTGTCTCTGCAGGCATGCGGTCCCATTGTGAAATAGCTGTGTTCATCGATGGACCC CTGGCTCTGGCAGATGGAATACCCTTCTTCCGCTCTGCCAATGGGGTGATTCTGACTCCAGGGAATACTG ATGGCTTCCTCCTTCCCAAGTACTTCAAGGAGGCCCTGCAGCTACGCCCTACCCGAAAGCCCCTTTCCTT GGCTGGTGATGAAGAGACAGAGTGTCAGAGTAGCCCCAAGCACAGCTCCAGAGAAAGGAGGAGGATCCAA CAATAAAATATTAATTTATAAAAAAGAAATTTTAAAAAGTAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026364 unedited GGATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCCTGTCAT GTCCACAGCAGACTGAGGAGAGTTCTTGTCTGGGCCAGGCCTGGTGACCGCCCCCTCCAG CCAAGCAGCCAGATCAGCACTTACCCCGAGGGCGAGCCCTGGAGGGTACGGTGCAGCTGG TGGTGTGAGTGTGCACTCCGGGCCAGCGCTATGCTGGGCACTCACCTCCCTGCCCCTCCG TGAGGCAGATCCTCTTTTTATATGGCTTCGTGCCCCTGGGCACCCTCCTGCAGTTGCCCC AGTTCCGCGGCTTCTCTGCTGAAGATGTGCAGCGCGTGGTGGACACCAATAGGAAGCAGC GGTTCGCCCTGCAGCTGGGGGATCCCAGCACTGGCCTTCTCATCCGGGCCAACCAGGGCC ATTCCCTGCAGGTACCTAAGTTGGAGCTGATGCCCCTGGAGACACCGCAGGCCCTGCCCC CGATGCTAGTCCATGGTACATTCTGGAAGCACTGGCCATCCATCCTACTCAAAGGCCTGT CCTGCCAGGGAAGGACGCACATTCACCTGGCCCCAGGACTGCCTGGAGACCCCGGTATCA TCAGTGGTCAGTGCCCTCCCCTCCACCTAGCTACTCCCACCCACTCTGTCCTCCCAGGTC CCCTTCAAAGGTTACAACACTTGTCTGGNGCCACCCCATGTCCGGCATGGAGCAGCACCC CTGCATCCNCCAGCCCAGNACCCCAGTCTGCCTAGGTTGCTGACTGCACTNNCAGTGCCC NAGCCTCAGCTCCAGCTGTCTCTGCAGGCATGCGGTCCCATTGTGAATAGCTGTNGTCAT CGATGGACCCCTGGCTCTGGCAGATGGNATACCCTTCTTNNCGCTCTGCATGGNNGTGAT NCTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK026364 unedited CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTGTTACTTTTTAAAATTTC TTTTTTATAAATTAATATTTTATTGTTGGATCCTCCTCCTTTCTCTGGAGCTGTGCTTGG GGCTACTCTGACACTCTGTCTCTTCATCACCAGCCAAGGAAAGGGGCTTTCGGGTAGGGC GTAGCTGCAGGGCCTCCTTGAAGTACTTGGGAAGGAGGAAGCCATCAGTATTCCCTGGAG TCAGAATCACCCCATTGGCAGAGCGGAAGAAGGGTATTCCATCTGCCAGAGCCAGGGGTC CATCGATGAACACAGCTATTTCACAATGGGACCGCATGCCTGCAGAGACAGCTGGAGCTG AGGCCTGCGCACCTGNAGTGCAGTCAGCAACCTAGGCAGACTGGGGTCCTGGGCTGGGGG ATGCAGGGGTGCTGCTCCATGCCGGACATGGGGTGGCCCCAGACAAGTGTTGTAACCTTT GAAGGGGACCTGGGAGGACAGAGTGCGTGGGAGTACCTACGTGGAGGGGAGGCCACTGAC CCCTGATGATCCCGGGGTCTCAGGCAGCCCTGGGGCCAGTGAATGGGCGCCTTCCCTGGC AGACGGCCTTTGGATAGATGGATGCCCGTGCTTCCAAACGTACCTGAACTACCATTCGGG CCAGGCCCGGGGGGTCCCCAGGGCCACAGTTCCACTTAAGTACCCGACGGAATGGCCCTG CTTGCCCCGAAGAAAGGCCAAGGCTGGAACCCCCCCTGCAGGGCAAACGCTTGCTCTTAT GGTGTCCACCCCCCCTGCACTCTTAAAAAATAACCCCGGACCGGGCACCCGCAGAAGGGC CCAGGGGCCCAACCCTTAAAAGAAGACCCCTCACCGAGGGCCGGAGGGAACTCCACTAGG TGGCCGGAGGACACTCACCCCTGTGCCCGCCCTCCGGTTCCCTTGGAAAGGTCACTCCCC TTGTTGGGGGGGCGTCAACCCTGCCCAAAAAATCT
Restriction Sites	NotI-NotI
ACCN	AK026364
Insert Size	1130 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK026364.1
RefSeq Size	1738 bp
RefSeq ORF	1738 bp
Locus ID	83707
Gene Summary	Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.[UniProtKB/Swiss-Prot Function]

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