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CHIT1 (NM_003465) Human Untagged Clone
CAT＃: SC117950
CHIT1 (untagged)-Human chitinase 1 (chitotriosidase) (CHIT1)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHI3; CHIT; CHITD |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117950 sequence for NM_003465 edited (data generated by NextGen Sequencing)
ATGGTGCGGTCTGTGGCCTGGGCAGGTTTCATGGTCCTGCTGATGATCCCATGGGGCTCT GCTGCAAAACTGGTCTGCTACTTCACCAACTGGGCCCAGTACAGACAGGGGGAGGCTCGC TTCCTGCCCAAGGACTTGGACCCCAGCCTTTGCACCCACCTCATCTACGCCTTCGCTGGC ATGACCAACCACCAGCTGAGCACCACTGAGTGGAATGACGAGACTCTCTACCAGGAGTTC AATGGCCTGAAGAAGATGAATCCCAAGCTGAAGACCCTGTTAGCCATCGGAGGCTGGAAT TTCGGCACTCAGAAGTTCACAGATATGGTAGCCACGGCCAACAACCGTCAGACCTTTGTC AACTCGGCCATCAGGTTTCTGCGCAAATACAGCTTTGACGGCCTTGACCTTGACTGGGAG TACCCAGGAAGCCAGGGGAGCCCTGCCGTAGACAAGGAGCGCTTCACAACCCTGGTACAG GACTTGGCCAATGCCTTCCAGCAGGAAGCCCAGACCTCAGGGAAGGAACGCCTTCTTCTG AGTGCAGCGGTTCCAGCTGGGCAGACCTATGTGGATGCTGGATACGAGGTGGACAAAATC GCCCAGAACCTGGATTTTGTCAACCTTATGGCCTACGACTTCCATGGCTCTTGGGAGAAG GTCACGGGACATAACAGCCCCCTCTACAAGAGGCAAGAAGAGAGTGGTGCAGCAGCCAGC CTCAACGTGGATGCTGCTGTGCAACAGTGGCTGCAGAAGGGGACCCCTGCCAGCAAGCTG ATCCTTGGCATGCCTACCTACGGACGCTCCTTCACACTGGCCTCCTCATCAGACACCAGA GTGGGGGCCCCAGCCACAGGGTCTGGCACTCCAGGCCCCTTCACCAAGGAAGGAGGGATG CTGGCCTACTATGAAGTCTGCTCCTGGAAGGGGGCCACCAAACAGAGAATCCAGGATCAG AAGGTGCCCTACATCTTCCGGGACAACCAGTGGGTGGGCTTTGATGATGTGGAGAGCTTC AAAACCAAGGTCAGCTATCTGAAGCAGAAGGGACTGGGCGGGGCCATGGTCTGGGCACTG GACTTAGATGACTTTGCCGGCTTCTCCTGCAACCAGGGCCGATACCCCCTCATCCAGACG CTACGGCAGGAACTGAGTCTTCCATACTTGCCTTCAGGCACCCCAGAGCTTGAAGTTCCA AAACCAGGTCAGCCCTCTGAACCTGAGCATGGCCCCAGCCCTGGACAAGACACGTTCTGC CAGGGCAAAGCTGATGGGCTCTATCCCAATCCTCGGGAACGGTCCAGCTTCTACAGCTGT GCAGCGGGGCGGCTGTTCCAGCAAAGCTGCCCGACAGGCCTGGTGTTCAGCAACTCCTGC AAATGCTGCACCTGGAATTGA Clone variation with respect to NM_003465.2 >OriGene 5' read for NM_003465 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCTGAGCTGCATCATGGT GCGGTCTGTGGCCTGGGCAGGTTTCATGGTCCTGCTGAGATCCCTGGGGCTCTGCTGCAA AACTGGTCTGCTACTTCACCAACTGGGCCCAGTACAGACAGGGGGAGGCTCGCTTCCTGC CCAAGGACTTGGACCCCAGCCTTTGCACCCACCTCATCTACGCCTTCGCTGGCATGACCA ACCACCAGCTGAGCACCACTGAGTGGAATGACGAGACTCTCTACCAGGAGTTCAATGGCC TGAAGAAGATGAATCCCAAGCTGAAGACCCTGTTAGCCATCGGAGGCTGGAATTTCGGCA CTCAGAAGTTCACAGATATGGTAGCCACGGCCAACAACCGTCAGACCTTTGTCAACTCGG CCATCAGGTTTCTGCGCAAATACAGCTTTGACGGCCTTGACCTTGACTGGGAGTACCCAG GAAGCCAGGGGAGCCCTGCCGTAGACAAGGAGCGCTTCACAACCCTGGTACAGGACTTGG CCAATGCCTTCCAGCAGGAAGCCCAGACCTCAGGGAAGGAACGCCTTCTTCTGAGTGCAG CGGTTCCAGCTGGGCAGACCTATGTGGATGCTGGATACGAGGTGGACANAATCGCCCAGA ACCTGNATTNTGTCAACCTTATGGCCTACGACTTCCATGGGGCTCTGGAGAAGTCACGGG ACATAACAGCCCCCTCTACAAAGAGCAGAAGAGAGTGTGCAGCAGCCAGCCTCACGNTGA TGCTGCTGTGCACAGTGGCTGCAAAAGGGGACCCCTGNNCACAGCTGATCCTTGCATGCC TACTACNGACGCTCTTCAACTGGNCTNCTATCAACACCAAAGTGGGGGCCCAGCCACAGG TCTGCACTTNCAGCCCTTCACANGNANNGAGGNAGCTGNCTACCTATGAACTGCTCTGAA GGGGNNCACAACAAGAATCAGATCGAAGTGCCTAACTCTCGAAACCATNGNTGGCTTGTA ATGNGAAGCTCAANCGGTCACTTTGACAAGGATGGCGGGCTGNTG >OriGene 3' read for NM_003465 unedited
GTCGAGTTAAATCCTTTTTTTTTTTGAAGAGGGGCACAAACCAAAGATTTATTTTGCAAG TGAAAGGGGCAGCCCAGGAGACCCAGAAAAAAGGAAGGCAAGGCTGAGAGCAGAAAGCCT GGATAAAGGAAGACCACAGAAAGGCCTGCAGGAGCCAGATTGCGGCCCCCAGGGAAAACC CAGGAGGCAGGCTGTAGAGTGATCCTGGGCCCAGCCTCAAAGCTGGGACTGGAGGGGCTT TAGCGACTCAATTCCAGGTGCAGCATTTGCAGGAGTTGCTGAACACCAGGCCTGTCGGGC AGCTTTGCTGGAACAGCCGCCCCGCTGCACAGCTGTAGAAGCTGGACCGTTCCCGAGGAT TGGGATAGAGCCCATCAGCTTTGCCCTGGCAGAACGTGTCTTGTCCAGGGCTGGGGCCAT GCTCAGGTTCAGAGGGCTGACCTGGTTTTGGAACTTCAAGCTCTGGGGTGCCTGAAGGCA AGTATGGAAGACTCAGTTCCTGCCGTAGCGTCTGGATGAGGGGGTATCGGCCCTGGTTGC AGGAGAAGCCGGCAAAGTCATCTAAGTCCAGTGCCCAGACCATGGCCCCGCCCAGTCCCT TCTGCTTCAGATAGCTGACCTTGGTTTTGAAGCTCTCCACATCATCAAAGCCCACCCACT GGTTGTCCCGGAAGATGTANGGCACCTTCTGATCCTGGATTCTCTGTTTGGTGGCCCCCT TCCAGGAGCAGACTTCATAGTAGGCCAGCATCCCTCCTTCCTTGGTGAAGGGGCCTGGNA GTGCAGACCCTGTGGCTGGGGCCCCCACTCTGGTGTCTGATGAGGGAGCCAGTGTGAAAG AACCTCCGTTAGTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003465 |
| Insert Size | 1750 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003465.1, NP_003456.1 |
| RefSeq Size | 1633 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 1118 |
| UniProt ID | Q13231 |
| Domains | Glyco_18, CBM_14 |
| Protein Families | Secreted Protein, Transmembrane |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism |
| Gene Summary | Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) is the predominant transcript and it encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. A downstream translational start codon is selected for this RefSeq based on its better conservation in mammalian species and on the presence of a predicted signal peptide in the protein N-terminus. An upstream in-frame start codon is also present but is poorly conserved, and use of the upstream start codon would result in a protein that is 10 aa longer at the N-terminus and lacks a predicted signal peptide. Leaky scanning by ribosomes may allow translation initiation at the downstream start codon. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219169 | CHIT1 (Myc-DDK-tagged)-Human chitinase 1 (chitotriosidase) (CHIT1) |
CNY 3,656.00 |
|
| RC219169L1 | Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219169L2 | Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), mGFP tagged |
CNY 5,890.00 |
|
| RC219169L3 | Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219169L4 | Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), mGFP tagged |
CNY 5,890.00 |
|
| RG219169 | CHIT1 (tGFP-tagged) - Human chitinase 1 (chitotriosidase) (CHIT1) |
CNY 4,370.00 |
