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ATP6V0E1 (NM_003945) Human Untagged Clone
CAT＃: SC111093
ATP6V0E1 (untagged)-Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1)
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ATP6H; ATP6V0E; M9.2; Vma21; Vma21p |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111093 sequence for NM_003945 edited (data generated by NextGen Sequencing)
ATGGCGTATCACGGCCTCACTGTGCCTCTCATTGTGATGAGCGTGTTCTGGGGCTTCGTC GGCTTCTTGGTGCCTTGGTTCATCCCTAAGGGTCCTAACCGGGGAGTTATCATTACCATG TTGGTGACCTGTTCAGTTTGCTGCTATCTCTTTTGGCTGATTGCAATTCTGGCCCAACTC AACCCTCTCTTTGGACCGCAATTGAAAAATGAAACCATCTGGTATCTGAAGTATCATTGG CCTTGA Clone variation with respect to NM_003945.3 >OriGene 5' read for NM_003945 unedited
GCACGAGGGCCGACCATGGTCGTATCACGGCCTCACTGTGCCTCTCATTGTGATGAGCGT GTTCTGGGGCTTCGTCGGCTTCTTGGTGCCTTGGTTCATCCCTAAGGGTCCTAACCGGGG AGTTATCATTACCATGTTGGTGACCTGTTCAGTTTGCTGCTATCTCTTTTGGCTGATTGC AATTCTGGCCCAACTCAACCCTCTCTTTGGACCGCAATTGAAAAATGAAACCATCTGGTA TCTGAAGTATCATTGGCCTTGAGGAAGAAGACATGCTCTACAGTGCTCAGTCTTTGAGGT CACGAGAAGAGAATGCCTTCTAGATGCAAAATCACCTCCAAACCAGACCACTTTTCTTGA CTTGCCTGTTTTGGCCATTAGCTGCCTTAAACGTTAACAGCACATTTGAATGCCTTATTC TACAATGCAGCGTGTTTTCCTTTGCCTTTTTTGCACTTTGGTGAATTACGTGCCTCCATA ACCTGAACTGTGCCGACTCCACAAAACGATTATGTACTCTTCTGAGATAGAAGATGCTGT TCTTCTGAGAGATACGTTACTCTCTCCTTGGAATCTGTGGATTTGAAGATGGCTCCTGCC TTCTCACGTGGGAATCAGTGAAGTGTTTAGAAACTGCTGCAAGACAAACAAGACTCCAGT GNGGTGGTCAGTAGGAGAGCACGTTCAGAGGGAA >OriGene 3' read for NM_003945 unedited
TTGAACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTCCATAAAAAGGAGGAAAATATTTATTCCAAAAGAATGGCAAAAAAAAAAAAT TCATCCTGAAAGTATAGTTGGGGGCGATTCTGTTGAAATGGCTTTTCCTTTTGAACGTGC TTTCCTACTGACCACCCCACTGGAGTCTTGTTTGTCTTGCAGCAGTTTCTAAACACTTCA CTGATTCCCACGTGAAAAGGCAGGAGCCATTTTCAAATCCACAGATTCCAAGGAAAGAGT AACGTATTTTTCAAAAAAACAGCATTTTTTATTTCAAAAAAGTACATAATCGTTTTGGGG AGTCGGCACAGTTCAGGTTATGGAGGCACGTAATTCACCAAAGTGCAAAAAAGGCAAAGG AAAACACCCTGCATTGTAAAATAAGGCATTCAAATGTGCTGTTAACGTTTAAGGCAGCTA ATGGCCAAAACAGGCAAGTCAAAAAAAGTGGTCTGGTTTGGAGGTGATTTTGCATCTAAA AGGCATTCTCTTCTCGTGACCTCAAAAACTGACCACTGTAAAGCATGTCTTCTTCCTCAA GGCCAATGATACTTCAAATACCAAATGGTTTCATTTTTCAATTGCGGTCCAAAGAGAGGG TTGAGTTGGGCCAAAATTGCAATCAGCCAAAAGAGATAGCAGCAAACTGAACAGGTCACC AACATGGTAATGATAACTCCCCGGTTAGGACCCTTAGGGATGAACCAGGCACCAAGAAGC CGACGAAGCCCCAGACACGCTCATCACAATGAGAGGCCAGTGAGGCCGTGATCGCATGGT CGCCTCGTGCCGATTCCGGCCGCCTATAGTGAGTCGTATACAAAATCTGACGTTCACTAA CGAGCTTGCTTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003945 |
| Insert Size | 880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003945.3, NP_003936.1 |
| RefSeq Size | 894 bp |
| RefSeq ORF | 246 bp |
| Locus ID | 8992 |
| UniProt ID | O15342 |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215618 | ATP6V0E1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1) |
CNY 1,200.00 |
|
| RC215618L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215618L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1), mGFP tagged |
CNY 5,890.00 |
|
| RG215618 | ATP6V0E1 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1 (ATP6V0E1) |
CNY 2,800.00 |
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