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PIGA (NM_020473) Human Untagged Clone
CAT＃: SC109568
PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3
CNY 1,200.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPI3; MCAHS2; PIG-A; PNH1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_020473, the custom clone sequence may differ by one or more nucleotides
ATGGAACTCACCGGGATCGATTTGCTTAGTGGTATAATACCTGAACTCTGTCAGAAATATCCAGATTTAA ATTTCATAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGGAAGAAGTTCGGGAAAGATACCAGCTGCA TGACAGGGTGCGTCTTTTGGGAGCTTTAGAACACAAGGATGTTAGAAATGTCTTAGTTCAAGGACATATT TTTCTGAATACCTCCCTTACTGAAGCATTCTGCATGGCGATCGTGGAAGCAGCCAGTTGTGGTTTACAGG TTGTAAGTACCAGAGTTGGTGGAATTCCTGAGGTGCTTCCAGAAAACCTTATTATTTTATGTGAGCCTTC AGTAAAATCTTTGTGTGAAGGATTGGAAAAGGCTATTTTCCAACTGAAGTCAGGGACATTGCCAGCTCCA GAAAACATCCATAACATAGTAAAGACTTTCTACACCTGGAGGAATGTTGCAGAAAGAACTGAAAAGGTAT ATGACCGGGTATCAGTGGAAGCTGTGTTGCCAATGGACAAACGACTGGACAGACTTATTTCTCACTGCGG CCCAGTAACAGGCTACATCTTTGCTTTGTTGGCAGTTTTCAACTTCCTCTTCCTCATTTTCTTGAGATGG ATGACTCCAGATTCTATCATTGATGTTGCAATAGATGCCACTGGGCCACGGGGTGCCTGGACTAATAACT ATTCTCACAGTAAAAGAGGGGGTGAGAATAATGAGATATCTGAAACCAGGTAG >OriGene 5' read for NM_020473 unedited
GTTCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGCATTCGGCACGAGGGGGAAC TCACCGGTAATAGAGGACACATCTCTTAACTGGGTTGCTCTAAGAACTGATGTCTAAACC GTCTCAGCATGGCCTGTAGAGGAGGAGCTGGGAATGGCCACCGTGCCTCAGCTACACTCT CTCGGGGATCGATTTGCTTAGTGGTATAATACCTGAACTCTGTCAGAAATATCCAGATTT AAATTTCATAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGGAAGAAGTTCGGGAAAG ATACCAGCTGCATGACAGGGTGCGTCTTTTGGGAGCTTTAGAACACAAGGATGTTAGAAA TGTCTTAGTTCAAGGACATATTTTTCTGAATACCTCCCTTACTGAAGCATTCTGCATGGC GATCGTGGAAGCAGCCAGTTGTGGTTTACAGGTTGTAAGTACCAGAGTTGGTGGAATTCC TGAGGTGCTTCCAGAAAACCTTATTATTTTATGTGAGCCTTCAGTAAAATCTTTGTGTGA AGGATTGGAAAAGGCTATTTTCCAACTGAAGTCAGGGACATTGCCAGCTCCAGAAAACAT CCATAACATAGTAAAGACTTTCTACACCTGGAGGAATGTTGCAGAAAGAACTGAANAGGT ATATGACCGGGTATCAGTGGAAGCTGTGTTGCCAATGGACAAACGACTGGACAGACTTAT TTCTCACTGCGGCCCAGTAACAGGCTACATCTTTGCTTTGTTGGCAGTTTTCAACTTCCT CTTCCTCATTTTCTTGAGATGGATGACNTCCGATTCTATCATTGATGGTGCAATAGATGC CACTGGGCCCGCGGNGTGCCTGGACTAATAACTATTCTCACAGTAAAGAAGGGGGTGAGC ATTATGAGATATCTGAAACCAGGTAGAAG >OriGene 3' read for NM_020473 unedited
TATGACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATCAAAATGTCAT TCTGGTCTTTATTTTTGGACATGTAGCATGTTTTAACAAATCAGTTTTTCATAGGCAACC TTTTGAAACATCAAAAGAAATACAATATATTTTTCACAAATTTCTCATCACTGTAAATTC ACTTTAAAATCAACTAAGTAGCAGGGTAGAGCAATGTGTTTCCATATACCGACCAGTGCA AACTGACAGTTACAATTAAGGTATCCAGAAAGGTTGAAAATTAAGATTTACGTGAAACAA ATTTACTTCCATTTGTTAAAAAAAAGAAAAATAATAATTTGCAAATCACATGTTTTACAG GCAAAGGCAACTAACTTTAGATCTTATTTCCATCTCAAATAAATGACAGTTTAAGAATTA AATGACAATTTAAAAAAATTAAGCAAAAATATAAGGAAAAAATGGTGGCATGCCTCTAAA ACCTGTTGAATAGAATAATGGCCAAATATTACAGTTTCTCACTTTCCTATGAATACTGGC ACTGTTTATTTCATGTTTATATGTGAGTTTCTATGCATAAAAATCCCAGTAAGACTGAAT AGTTTAAAGATCAGTCCATTTTTCTCCAACAAAACCCCTAACTTCTAGATTTTAAAAATT GCACTCTTGCTTTTGCATTNTAGTACAAACAAAACACAAATATATTTCTTTTATATCAGT GCAACCAGTTAATAGGCATGTTATTTTTTAAAAGTAATAAATTTTGTGAATGGTGTCATT NTTAAAATCCTTACAGAATCACACACTGTCAGATCTATGTAGTGCACCCACAGTGCCTTA TTGTTTGAATTACAGTCCTTTCTCACAAACCTTAGAGTNCAAGATCATATACCACATGTG ATAATTGGGGTAAAAACCATGCTCTGGCACACATGACAGTATACATCCCCCAGACGNTCT AAATTCACTAGTGCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_020473 |
| Insert Size | 3090 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020473.1, NP_065206.1 |
| RefSeq Size | 2867 bp |
| RefSeq ORF | 81 bp |
| Locus ID | 5277 |
| UniProt ID | P37287 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| Gene Summary | This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and uses an alternate start codon, compared to variant 1. The resulting isoform (3) has a distinct and shorter N-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207733 | PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 3,990.00 |
|
| RC207733L3 | Lenti-ORF clone of PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 5,890.00 |
|
| RC207733L4 | Lenti-ORF clone of PIGA (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 5,890.00 |
|
| RG207733 | PIGA (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 4,370.00 |
|
| SC317265 | PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 3,990.00 |
