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PIGA (NM_020473) Human Tagged ORF Clone
CAT＃: RG207733
- TrueORF®
PIGA (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3
ORF Plasmid: DDK
"NM_020473" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GPI3; MCAHS2; PIG-A; PNH1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207733 representing NM_020473
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTCACCGGGATCGATTTGCTTAGTGGTATAATACCTGAACTCTGTCAGAAATATCCAGATTTAA ATTTCATAATTGGAGGAGAGGGACCAAAGAGAATCATTTTGGAAGAAGTTCGGGAAAGATACCAGCTGCA TGACAGGGTGCGTCTTTTGGGAGCTTTAGAACACAAGGATGTTAGAAATGTCTTAGTTCAAGGACATATT TTTCTGAATACCTCCCTTACTGAAGCATTCTGCATGGCGATCGTGGAAGCAGCCAGTTGTGGTTTACAGG TTGTAAGTACCAGAGTTGGTGGAATTCCTGAGGTGCTTCCAGAAAACCTTATTATTTTATGTGAGCCTTC AGTAAAATCTTTGTGTGAAGGATTGGAAAAGGCTATTTTCCAACTGAAGTCAGGGACATTGCCAGCTCCA GAAAACATCCATAACATAGTAAAGACTTTCTACACCTGGAGGAATGTTGCAGAAAGAACTGAAAAGGTAT ATGACCGGGTATCAGTGGAAGCTGTGTTGCCAATGGACAAACGACTGGACAGACTTATTTCTCACTGCGG CCCAGTAACAGGCTACATCTTTGCTTTGTTGGCAGTTTTCAACTTCCTCTTCCTCATTTTCTTGAGATGG ATGACTCCAGATTCTATCATTGATGTTGCAATAGATGCCACTGGGCCACGGGGTGCCTGGACTAATAACT ATTCTCACAGTAAAAGAGGGGGTGAGAATAATGAGATATCTGAAACCAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207733 representing NM_020473
Red=Cloning site Green=Tags(s) MELTGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHDRVRLLGALEHKDVRNVLVQGHI FLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAP ENIHNIVKTFYTWRNVAERTEKVYDRVSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRW MTPDSIIDVAIDATGPRGAWTNNYSHSKRGGENNEISETR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020473 |
| ORF Size | 750 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020473.3, NP_065206.3 |
| RefSeq Size | 2867 bp |
| RefSeq ORF | 753 bp |
| Locus ID | 5277 |
| UniProt ID | P37287 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| Gene Summary | This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207733 | PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 3,990.00 |
|
| RC207733L3 | Lenti-ORF clone of PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 5,890.00 |
|
| RC207733L4 | Lenti-ORF clone of PIGA (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 5,890.00 |
|
| SC109568 | PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 1,200.00 |
|
| SC317265 | PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
CNY 3,990.00 |
