Dync1i2 (NM_001270624) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Dnci2; Dncic2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217023 representing NM_001270624 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGACAAAAGTGAATTAAAAGCTGAGTTGGAACGTAAGAAGCAACGGTTGGCCCAAATCAGAGAAG AAAAGAAGAGGAAAGAAGAGGAAAGGAAAAAAAAAGAAACTGATCAGAAGAAGGAAGCTGCAGTCTCTGT GCAGGAAGAGTCTGACCTTGAAAAGAAGAGAAGAGAAGCCGAAGCCTTGCTTCAGAGCATGGGGCTAACT ACAGACTCCCCAATTGTCCCTCCTCCCATGTCTCCATCCTCCAAGTCGGTGAGCACGCCAAGTGAAGCTG GAAGCCAGGACTCTGGAGATGGCGCCGTGGGATCTAGGACGCTGCACTGGGATACAGATCCATCAGCTCT TCAGCTCCACTCAGATTCCGATTTGGGACGAGGACCTATTAAACTTGGAATGGCCAAAATTACTCAAGTC GACTTTCCCCCTCGAGAAATCGTCACATATACAAAGGAAACTCAGACCCCGGTTACAGCTCAACCCAAAG AAGATGAGGAGGAGGAAGATGACGTAGCAGCTCCTAAGCCTCCTGTTGAACCTGAAGAAGAGAAAATTTT AAAAAAAGACGAAGAGAATGATAGCAAAGCTCCCCCTCATGAACTAACTGAAGAAGAAAAGCAACAAATT TTACACTCAGAAGAATTTTTAAGTTTCTTTGACCATTCTACAAGAATTGTAGAAAGAGCCCTCTCTGAGC AGATTAACATCTTCTTTGACTACAGTGGGAGAGATTTGGAAGACAAAGAAGGGGAGATTCAAGCAGGTGC TAAGCTGTCATTAAATCGACAGTTTTTTGATGAGCGTTGGTCAAAGCATCGAGTTGTTAGTTGCTTGGAT TGGTCATCCCAGTATCCAGAATTACTTGTGGCTTCCTATAATAACAATGAAGAGGCTCCTCATGAGCCTG ATGGTGTGGCCCTCGTATGGAATATGAAGTACAAGAAAACTACCCCAGAGTACGTGTTCCACTGCCAGTC AGCTGTAATGTCTGCTACATTTGCAAAATTTCATCCAAATCTTGTTGTTGGAGGTACATACTCAGGCCAA ATTGTGCTTTGGGATAACCGTAGCAATAAAAGAACCCCCGTCCAGAGAACACCACTGTCAGCTGCTGCAC ACACACACCCTGTCTACTGTGTAAATGTTGTTGGAACACAAAATGCTCATAATCTGATTAGCATCTCTAC TGATGGAAAAATTTGCTCCTGGAGCTTAGACATGCTTTCCCATCCACAGGATAGCATGGAGTTGGTTCAT AAACAGTCAAAGGCAGTAGCTGTAACATCTATGTCTTTCCCTGTTGGAGACGTCAACAACTTTGTTGTGG GCAGTGAAGAAGGTTCTGTGTATACAGCATGCCGCCATGGCAGCAAAGCCGGAATCAGTGAGATGTTTGA GGGACACCAGGGACCCATCACGGGAATCCACTGTCACGCAGCTGTTGGCGCAGTAGACTTCTCACATCTG TTCGTCACTTCGTCTTTTGACTGGACAGTAAAACTTTGGACAACTAAGAATAACAAGCCTTTGTACTCAT TTGAAGATAATTCAGACTATGTCTATGATGTTATGTGGTCACCCACCCACCCAGCCCTGTTTGCCTGTGT GGATGGCATGGGGAGACTGGATCTATGGAATCTCAACAATGACACAGAGGTGCCGACTGCCAGCATTTCT GTGGAGGGCAACCCTGCCCTTAACCGTGTGAGATGGACCCACTCGGGAAGAGAGATCGCCGTGGGTGATT CTGAAGGACAGATTGTCATATATGATGTGGGCGAGCAGATCGCTGTTCCCCGAAATGATGAATGGGCAAG ATTTGGCCGAACACTCGCAGAGATTAACGCAAGCCGAGCTGATGCAGAGGAGGAAGCGGCTACCCGGATT CCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217023 representing NM_001270624 Red=Cloning site Green=Tags(s) MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAAVSVQEESDLEKKRREAEALLQSMGLT TDSPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSALQLHSDSDLGRGPIKLGMAKITQV DFPPREIVTYTKETQTPVTAQPKEDEEEEDDVAAPKPPVEPEEEKILKKDEENDSKAPPHELTEEEKQQI LHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLD WSSQYPELLVASYNNNEEAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQ IVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVH KQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHL FVTSSFDWTVKLWTTKNNKPLYSFEDNSDYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASIS VEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINASRADAEEEAATRI PA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270624
ORF Size	1896 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270624.1, NP_001257553.1
RefSeq Size	2803 bp
RefSeq ORF	1899 bp
Locus ID	116659
MW	70.6 kDa
Gene Summary	intermediate chain of cytoplasmic dynein, a retrograde microtubule motor thought to be involved in organelle transport [RGD, Feb 2006]

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