Nat1 (NM_001037316) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nat
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR216258 representing NM_001037316 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATCGAAGCATACTTCGAAAGGATTGGTTACAAGAACTCAGTGAATAAGTTGGACTTGGCCACAT TAACTGAAGTTCTTCAACACCAGATGCGAGCAGTTCCTTTTGAGAATCTTAGTATGCACTGTGGAGAAGC CATGTGTCTGGGCTTAGAGGCCACTTTTGACCACATAGTAAGGAAAAAGCGGGGTGGGTGGTGTCTCCAG GTTAATCATCTGCTGTACTGGGCTCTGACCAAAATGGGTTTTGAAACCACAATGTTGGGAGGATATGTTT ACATAACTCCAGTCAACAAATATAGCAGTGAAATGGTCCACCTTCTAGTACAAGTGACCATCAGTGACAG GAACTACATTGTGGATTCTGCCTATGGAAGCTCCTACCAGATGTGGGAGCCTCTGGAATTAACCTCAGGG AAGGATCAGCCTCAGGTGCCTGCCATCTTTCGTTTGACAGAAGAGAATGGAACCTGGTACTTGGACCAAA TCAGAAGAGAGCAGGATGTTCCAAACCAAGAGTTTGTTAACTCGGACCTCCTTGAAAAGAGCAAATATCG AAAAATCTATTCCTTTACTCTTGAGCCCCGCACTATTGAGGATTTTGAATATGTGAATACCTACCTTCAG ACATCGCCAGCCTCTGTGTTTGTAAGCACATCGTTCTGTTCCTTGCAGACCTCAGAAGGGGTTTGCTGTT TAATTGGTTCCACCCTTACAAGTAGGAGATTCAGTTATAAGGACAATGTAGATCTGGTTGAGTTTAAGAG TCTGACTGAGGAAGAAATAGAAGATGTACTGAAAACCACATTTGGCATTTCTTTGGAGAAAAAGTTTGTG CCCAAACATGGCGAACTCGTTTTTACTATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR216258 representing NM_001037316 Red=Cloning site Green=Tags(s) MDIEAYFERIGYKNSVNKLDLATLTEVLQHQMRAVPFENLSMHCGEAMCLGLEATFDHIVRKKRGGWCLQ VNHLLYWALTKMGFETTMLGGYVYITPVNKYSSEMVHLLVQVTISDRNYIVDSAYGSSYQMWEPLELTSG KDQPQVPAIFRLTEENGTWYLDQIRREQDVPNQEFVNSDLLEKSKYRKIYSFTLEPRTIEDFEYVNTYLQ TSPASVFVSTSFCSLQTSEGVCCLIGSTLTSRRFSYKDNVDLVEFKSLTEEEIEDVLKTTFGISLEKKFV PKHGELVFTI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001037316
ORF Size	870 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001037316.1, NP_001032393.1
RefSeq Size	1741 bp
RefSeq ORF	873 bp
Locus ID	116631
UniProt ID	P50297
MW	33.4 kDa
Gene Summary	enzyme which acetylates both arylamines and arylalkylamines [RGD, Feb 2006]

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