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Mid1 (NM_022927) Rat Tagged ORF Clone
CAT＃: RR215383
- TrueORF®
Mid1 (Myc-DDK-tagged ORF) - Rat midline 1 (Mid1), (10 ug)
"NM_022927" in other vectors (3)
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CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fxy; Midline1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR215383 representing NM_022927
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC CCTGTGCACATAGCCTCTGTTTCAACTGTGCCCACCGCATCCTGGTCTCTCACTGTGCCACCAACGAGCC TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCTCAGCCAGCGAGGTCTA GACGGGCTCAAGCGCAACGTCACCTTACAAAACATCATTGACAGGTTTCAGAAAGCATCCGTGAGTGGGC CCAATTCTCCCAGTGAGACCCGCAGGGAGCGGGCCTTTGACGCCAACACCATGTCTTCTGCCGAGAAAGT TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACCTGTGTTACTTGCGAAGTGTCC TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC CAATCCCGGACTCGCACATCCGGGGGCTAATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAATTTGGAGAGTAATCTCACCAATCTTATTAAGAGAA ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACTTGTCAACATGTTGAGGTCAATGCATCCCG TCAAGAAGCCAAGTTGACAGAGGAATGTGATCTTCTCATTGAAATTATTCAGGAAAGAAGACAAATTATT GGAACAAAGATTAAAGAAGGCAAGGTGATGAGGCTCCGCAAATTAGCTCAGCAGATTGCAAACTGTAAAC AATGCATTGAAAGGTCTGCATCACTCATTTCCCAAGCAGAACACTCACTGAAGGAGAATGACCATGCCCG TTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTCCTAATT CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGGGAGAAGAAACTGCTAG AATGTTTGGATTACCTAACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACGGCTTCCTATGA CACCATCACTGTCCACTGGACTTCGGATGATGAGTTCAGTGTGGTCTCCTATGAACTCCAGTACACCATA TTCACTGGACAAGCCAATGTCGTTAGTCTTTGTAACTCGGCAGATAGCTGGATGATTGTGCCCAACATCA AGCAGAACCACTATACTGTGCATGGCCTACAAAGTGGCACCAAGTATATCTTCATGGTCAAGGCCATCAA CCAGGCAGGCAGCCGCAGCAGTGAGCCTGGGAAGCTGAAGACAAACAGTCAGCCATTTAAACTGGATCCC AAATCTGCTCATCGAAAACTCAAGGTGTCCCATGACAACCTGACTGTTGAACGTGATGAGTCATCATCCA AAAAGAGTCACACACCTGAACGCTTCACTAGCCAAGGGAGCTATGGCGTGGCTGGAAATGTGTTTATTGA CAGTGGCCGTCATTACTGGGAAGTGGTCATAAGCGGAAGCACATGGTATGCCATTGGCCTTGCGTACAAA TCAGCTCCAAAACATGAATGGATTGGGAAGAATTCTGCTTCCTGGGCCCTTTGCCGCTGCAACAATAACT GGGTGGTGAGACATAACAGCAAGGAAATACCCATTGAACCAGCCCCCCACCCCCGGCGTGTAGGCATCCT GCTGGACTATGATAATGGCTCAATCGCTTTTTATGATGCTTTGAACTCCATCCACCTCTATACCTTCGAT GTGGCGCTTGCGCAGCCTGTGTGCCCCACCTTTACTGTGTGGAACAAGTGTTTGACGATTATAACTGGTC TTCCCATCCCAGACCATTTGGACTGTACAGAACAGCTACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215383 representing NM_022927
Red=Cloning site Green=Tags(s) METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNEPVESINAFQCPTCRHVITLSQRGL DGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMSSAEKVLCQFCDQDPAQDAVKTCVTCEVS YCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVA ALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQERRQII GTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLI PEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTI FTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDP KSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYK SAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHPRRVGILLDYDNGSIAFYDALNSIHLYTFD VALAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022927 |
| ORF Size | 2001 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022927.1, NP_075216.1 |
| RefSeq Size | 2262 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 54252 |
| UniProt ID | P82458 |
| MW | 75.2 kDa |
| Gene Summary | human homolog forms homodimers which associate with microtubules in the cytoplasm and mutations in gene are associated with Opitz syndrome [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN215383 | Mid1 (untagged ORF) - Rat midline 1 (Mid1), (10 ug) |
CNY 8,650.00 |
|
| RR215383L3 | Lenti ORF clone of Mid1 (Myc-DDK-tagged ORF) - Rat midline 1 (Mid1), (10 ug) |
CNY 10,360.00 |
|
| RR215383L4 | Lenti ORF clone of Mid1 (mGFP-tagged ORF) - Rat midline 1 (Mid1), (10 ug) |
CNY 11,500.00 |
