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Bub1 (NM_001106507) Rat Tagged ORF Clone
CAT＃: RR214148
- TrueORF®
Bub1 (Myc-DDK-tagged ORF) - Rat budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), (10 ug)
"NM_001106507" in other vectors (3)
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CNY 17,010.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214148 representing NM_001106507
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACCTCGGAAATGTCTTTCGTATGTTTGAAGCCCATATGCAAAGCTACAAGGGTAATGACCCAC TTGGAGAATGGGAAAGCTTTATGAAGTGGGTAGAAGAAAATTTTCCTGACAATAAAGAACACTTGGTGAC AATATTAGAACATTTAATGAAGGAATTCTTAGATAAGAAGAACTACCACAATGATTCAAGATTCATCAGT TATTGCTTAAAATTTGCTGAGTACAACAGTGACCGTCATCAGTTTTTTGAGTTTCTGTACAGCCAGGGAG TTGGAACCAAGTCATCATATCTATACCTGTCCTGGGCAGGGCATCTGGAAGCCCAGGGAGAGCAGCAGCA TGCCAGTGCTATTTTTCAAACAGGAATTCACAATCAGGCTGAACCTATGGAACTACTACAACAACAATAC AGGCTATTCCAGGCACGCCTTACTGGAACACATTTGCCAGATCAAGCTACAACCTCAGAACCTTTGCATA GTGCACAGATTTTAAACCAAGTTATGTTAACAAAGTCAAGTCCAGAAACAGAAAACTCAGCTTGCGTTCC TAGGAGTCAGGGTTCAGAATGTTCTGGTGTGGCACCTTCTACTTGTGACGAAAAGTCTAATATTAGGGAA CAAAGAGTGATCATGATTTCCAAGTCAGAATGCTCTGTCAGCTCATCTATGGCACCCAAGCCTGAAGCTC AGCAAGTTATGTACTGCAAAGAAAAGCTTATCCGTGGAGATTCGGAATTTTCATTTGAAGAGCTGAGAGC CCAGAAATACAATCAACGAAAGAAGCATGAACAGTGGGTTAGTGAAGACAGAAATTATATGAAAAGGAAA GAAGCAAATGCTTTTGAAGAGCAATTATTAAAACAGAAAATGGATGAACTTCACAAGAAATTGCATCAAG TGGTGGAATTGTCTCACAAGGACCTGCCTGCTTCTGAGAACAGGCCTGACGCTAATCTAGTATGTGTGGG ACAAAATACATGCTCCCACCAAGAACTGAGGGGTCTAAGTCTTTCATCCATCAATCAGCAGACCTCAGAG AACTCAGGAGAGAAACCACTGGAAGAACCTTCTGTTCCTCTTATGGTAAATGCTGTTAACAGCACTTTGC TGTTCCCAGCCATCAGCCTGCCATCTCTTCCTATTCCTGTAAGTGGCCAGTCAGAGACAGATTCCAGAGG TGTGAATCAAAGTATTCGTGAATTCATGACACAGAGTGGACCAGACAAAAAAGAAGTATGTGAAACAAAT AAAGTTGCCAACATTAATGACTTTCATACAACTCCAAACACATCATTGGGAATGGTTCAAGGAACACCAT GCAAAGTGCAGCCATCACCAACTGTCCACACCAAGGAAGCATTAGGTTTCATCATGGATATGTTTCAGGC TCCAACACTTCCTGACATTTCTGATGATAAAGATGAATGGCCATCTCTAGACCAAAATGAAGATGCATTT GAAGCCCAGTTTCAAAAAAATGCAGTATTTTCAGAGGACTGGGGAGTTAAAAAGATTATGACTTTATCAT CTGCTTTTCCTATTTTTGAAGACGGAAACAAAGAAAATTATGGCTTACCACAGCCTAAAAATAAGCCTTT AGGAGCTAGGACCTTTGGAGAACGATCTCTCAGCAAATGTTCATCAAGATCAAATGAAATGCCTCACACT GATGAGTTTATGGATGACTCAACAGTATGTGGTATTCGCTACAACAAAACCCTAGCTCCCAGTCCTAAAA GTATAGGAGACTTTACATCTGCTGCCCAGCTTTCATCTACACCATTCCACAAATTTCCAGCAGATCCATT GCAGATTCCAGAAGATAAAGAAAATGTGGTAGCCACACAATATACACATATGGCTTTGGATTCTTGTAAA GAAAACATGGTGGACCCCTCAAAAGACAGAAAGCTTGGTCTAATTCAAGAGAAAATTTCAGCATCTTTAT CCTGTCCTAGTCAGCCTGCCACAGGTGGTTTGCTCACCCAGGAAGCAGTGTTCAGCCTTGAGACTTATAA ATGCACAGGCACTGACCGTGCGATCATGGAAGACCTATCAGATGCCAGCGCTGGGCTCCAAGTTGAATGG GTGCAGACTAGTTCATTTGGAAATGTTAATGCTCCAAACTTTACTATTGAGAACCCATGGGATGATGAAT TGATTCTTAAACTTCTATCTGGGCTTTCTAAGCCAGTTACTTCCTATTCAAATACTTTTGAGTGGCAGAG TAAACTTCCAGCCATCAAGACCAAGACAGAATTCCAGTTGGGTTCTTTGCTGGTCTATGTGAATCACCTT CTTGGAGAAGGAGCCTTTGCTCAAGTCTTTGAAGCTATTCATGGAGATACCAAAAGTAAACAGAAATGTA TTTTGAAGGTGCAGAAACCTGCCAACTCCTGGGAATTCTACATTGGGATGCAGCTGATGGAAAGACTAAA GCCAGAAGTACATCACATGTTCATCAAGTTTTATTCTGCTCACTTATTCCAGAATGGCAGCATATTAGTA GGGGAGCTCTATAGCTATGGGACATTACTAAATGTCATTAACCTCTATAAAAATACCTCTGAGAAAGTGA TGCCCCAAGCTCTCGTCATTACTTTCGCTATCAGAATGCTTTACATGGTTGAACAAGTCCACAGCTGCGA AATCATTCATGGAGACATTAAGCCAGATAACTTCATACTAGGACACAGATTTTTGGAACAGGATGATGAA GACTTAGCTACCGGCTTGACATTGATTGACCTGGGTCAGAGTATAGATATGAAACTTTTCCCTAAAGGAA CTGTATTTACAGGAAAGTGTGAAACATCTGGTTTTCAGTGCCCTGAGATGCTCAGTAACAAGCCATGGAA CTACCAGGTTGATTACTATGGAGTTGCTGCATCAATATATTGTATGCTCTTTGGCACTTACATGAAAGTA AAAAATGAAGGAGGAGTCTGGAAACCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGATATGTGGGAGG AATTCTTTCACATCATGTTGAATATACCGGATTGTCATAATCTTCCATCTTTGGACTTTCTGAGACAAAA GATGAACAAGTTACTTGAACAACAATATTCCAACAAAATTAAGACCTTGCGCAATAGGCTAATTGTGCTT CTTTCAGAATATAAGCGTTCAAGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214148 representing NM_001106507
Red=Cloning site Green=Tags(s) MDNLGNVFRMFEAHMQSYKGNDPLGEWESFMKWVEENFPDNKEHLVTILEHLMKEFLDKKNYHNDSRFIS YCLKFAEYNSDRHQFFEFLYSQGVGTKSSYLYLSWAGHLEAQGEQQHASAIFQTGIHNQAEPMELLQQQY RLFQARLTGTHLPDQATTSEPLHSAQILNQVMLTKSSPETENSACVPRSQGSECSGVAPSTCDEKSNIRE QRVIMISKSECSVSSSMAPKPEAQQVMYCKEKLIRGDSEFSFEELRAQKYNQRKKHEQWVSEDRNYMKRK EANAFEEQLLKQKMDELHKKLHQVVELSHKDLPASENRPDANLVCVGQNTCSHQELRGLSLSSINQQTSE NSGEKPLEEPSVPLMVNAVNSTLLFPAISLPSLPIPVSGQSETDSRGVNQSIREFMTQSGPDKKEVCETN KVANINDFHTTPNTSLGMVQGTPCKVQPSPTVHTKEALGFIMDMFQAPTLPDISDDKDEWPSLDQNEDAF EAQFQKNAVFSEDWGVKKIMTLSSAFPIFEDGNKENYGLPQPKNKPLGARTFGERSLSKCSSRSNEMPHT DEFMDDSTVCGIRYNKTLAPSPKSIGDFTSAAQLSSTPFHKFPADPLQIPEDKENVVATQYTHMALDSCK ENMVDPSKDRKLGLIQEKISASLSCPSQPATGGLLTQEAVFSLETYKCTGTDRAIMEDLSDASAGLQVEW VQTSSFGNVNAPNFTIENPWDDELILKLLSGLSKPVTSYSNTFEWQSKLPAIKTKTEFQLGSLLVYVNHL LGEGAFAQVFEAIHGDTKSKQKCILKVQKPANSWEFYIGMQLMERLKPEVHHMFIKFYSAHLFQNGSILV GELYSYGTLLNVINLYKNTSEKVMPQALVITFAIRMLYMVEQVHSCEIIHGDIKPDNFILGHRFLEQDDE DLATGLTLIDLGQSIDMKLFPKGTVFTGKCETSGFQCPEMLSNKPWNYQVDYYGVAASIYCMLFGTYMKV KNEGGVWKPEGLFRRLPHLDMWEEFFHIMLNIPDCHNLPSLDFLRQKMNKLLEQQYSNKIKTLRNRLIVL LSEYKRSRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106507 |
| ORF Size | 3177 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106507.1, NP_001099977.1 |
| RefSeq Size | 4583 bp |
| RefSeq ORF | 3180 bp |
| Locus ID | 296137 |
| MW | 120.1 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214148 | Bub1 (untagged ORF) - Rat budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), (10 ug) |
CNY 17,010.00 |
|
| RR214148L3 | Lenti ORF clone of Bub1 (Myc-DDK-tagged ORF) - Rat budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), (10 ug) |
CNY 18,620.00 |
|
| RR214148L4 | Lenti ORF clone of Bub1 (mGFP-tagged ORF) - Rat budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae) (Bub1), (10 ug) |
CNY 20,430.00 |
