Taf2 (NM_133319) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TAFIIB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209940 representing NM_133319 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCCACCGGCTTAGAGTCCTCCAGGATGAACAGGAAGAAAGGAGACAAGGGCTTCGAAAGCCCAC GGCCGTACAAATTAACCCATCAGGTCGTCTGCATCAACAACATAAACTTCCAGAGAAAGTCTGTTGTGGG GTTTGTGGAGCTGACTATATTTCCTACGGTTGCAAACTTGAATAGAATCAAGCTGAACAGTAAACAGTGT AGAATATACCGAGTCAGGATCAATGACTTGGAGGCTGCTTTTATTTATAATGATCCAACCTTAGAAGTTT GTCACAGCGAATCAAAACAGAGAAACCTCAATTATTTTTCCAATGCGTATGCAGCTGCAGTCAGTGCTGT GGACCCTGATGCGGGGAACGGAGAACTCTGCATTAAGGTCCCCTCAGAGCTCTGGAAGCACGTCGATGAG TTAAAGGTCCTGAAGATACATATTAATTTTTCTTTGGATCAGCCAAAAGGAGGTCTTCATTTTGTGGTAC CCAGTGTAGAGGGGAGTATGGCAGAGAGAGGTGCTCATGTCTTCTCTTGTGGGTATCAGAATTCTACAAG ATTTTGGTTCCCTTGTGTTGATTCATACTCTGAGTTGTGTACCTGGAAATTAGAATTTACAGTCGATGCT GCGATGGTGGCTGTGTCCAATGGAGATTTGGTGGAGACAGTGTATACTCACGACATGAGAAAGAAGACCT TCCATTATGTGCTTGCTATTCCTACAGCAGCGTCCAATATCTCCTTGGCCATCGGGCCATTTGAAATACT TGTAGATCCATATATGCACGAGGTTACCCATTTTTGTTTGCCTCAACTTCTTCCATTACTTAAACATACT ACATCATACCTTCACGAAGTTTTTGAATTTTATGAGGAAATTTTGACTTGTCGCTATCCGTACTCCTGTT TTAAGACGGTCTTCATTGATGAGGCTTACGTGGAAGTGGCCGCTTATGCGTCTATGAGCATTTTCAGCAC AAATCTCCTGCACAGCGCCATGATTATAGATGAGACACCGCTCACCAGGCGGTGCCTCGCACAGTCTCTA GCACAGCAGTTCTTCGGATGTTTCATCTCCAGAATGTCTTGGTCTGATGAGTGGGTGCTGAAGGGAATTT CAGGTTATATTTATGGACTGTGGATGAAGAAAACGTTTGGTGTCAATGAGTACCACCATTGGATTAAAGA GGAACTCGATAAAATAGTGGCATATGAACTGAAAACTGGGGGAGTTTTACTGCATCCCATATTTGGTGGT GGAAAAGAAAAGGATAACCCCGCATCTCACCTCCACTTTTCAATAAAGCATCCACATACACTGTCTTGGG AATACTACACTATGTTTCAGTGTAAAGCTCACCTCGTGATGAGACTGATTGAAAACAGAATCAGCATGGA ATTTATGCTGCAAGTTTTCAATAAACTATTAAGTCTGGCCAGTACCGCTTCATCCCAGAAGTTCCAGTCA CATATGTGGAGCCAGATGCTGGTCTCCACCTACGGGTTTTTGAAGTCCATTTCCAATGTCTCTGGCAAAG ACATCCAGCCCTTAATAAAGCAGTGGGTAGACCAGAGTGGAGTGGTAAAGTTTTATGGAAGTTTTGCATT TAATAGAAAACGAAATGTTTTAGAACTAGAAATAAAACAAGATTACACATCTCCTGGCACCCAGAAGTAC GTGGGACCACTTAAAGTGACAGTGCAAGAGTTAGACGGCTCCTTCAACCATACCTTGCAAATTGAAGAAA ACAGCCTTAAACATGACATACCCTGCCACTCCAAAAGCAGAAGGAATAAGAAGAAGAAAATCCCACTGAT GAATGGGGAAGAAGTCGATATGGATCTTTCTGCAATGGATGCGGACTCCCCTTTGCTGTGGATAAGGATA GACCCAGATATGTCTGTCCTGAGGAAGGTGGAGTTTGAGCAGGCTGACTTTATGTGGCAGTATGAGCTCC GCTATGAGAGAGATGTTGTTGCTCAGCAGGAATCCATCTTGGCCTTGGAAAAGTTCCCCACGCCCGCATC GCGCCTCGCGCTCACTGATATACTAGAGCAAGAGCAGTGTTTCTACCGAGTGAGGATGTCAGCATGCTTC TGCCTTGCAAAGATTGCAAACTCAATGGTGAGCACATGGACGGGACCACCAGCCATGAAGTCCCTCTTTA CTAGAATGTTTTGTTGTAAAACTTGTCCCAACATCGTGAAAACAAACAACTTCATGAGCTTTCAGAGTTA TTTTCTACAGAAGACTATGCCAGTTGCAATGGCTTTGTTAAGAGACGTACATAATCTTTGTCCCAAAGAA GTTTTAACATTTATTTTAGACTTAATCAAGTACAATGACAACAGAAAAAATAAGTTTTCAGATAACTATT ACCGTGCAGAAATGATTGATGCCTTGGCTAACTCTGTCACACCTGCTGTCAGCGTGAATAATGAAGTCAG AACTTTGGATAACTTAAATCCTGATGTGCGACTAATACTTGAAGAAATTACCCGGTTTTTGAATATGGAA AAACTTCTCCCAAGTTACAGACATACTATCACTGTCAGTTGTTTGAGAGCCATAAGAGTGCTTCAGAAGA ATGGACATGTGCCAAGTGATGCATCTCTTTTCAAATCCTATGCTGAGTATGGCCACTTCGTGGACATCAG GATAGCGGCTCTGGAAGCCGTCGTCGATTACACCAAAGTGGACAGGAGTTATGAAGAACTTCAATGGCTA CTTAATATGATTCAGACTGACCCTGTCCCCTATGTGAGGCATAAGATTCTAAACATGCTGACTAAAAATC CGCCATTTACAAAGAACATGGAGTCTCCTTTATGCAACGAGGCCTTGGTAGATCAGCTCTGGAAGCTGAT GAATTCTGGTACTGCACATGACTGGAGGTTACGGTGTGGAGCTGTGGACTTGTACTTCACGCTCTTTGGC CTCAGTAGACCTTCTTGTTTACCCTTGCCAGAGCTCGGGCTTGTTCTTAACCTAAAAGAGAAGAAAGCTG TCTTGAATCCAACCATAATTCCAGAAGCGGGAGTAGGCAATCAGGAAACTGCAAGTAACCCAGGCTGTCA CACTCAGCTAGCAGGATTTCAGAACCCTTTCTCAAGTTCTCAGGATGAGGAGGAGGTCGATATGGACACC GTTCATGATAGTCAGGCCTTCATTTCCCATCATCTGAACATGCTTGAAAGGCCGTCCACTCCAGGGCTCT CTAAGTACCGACCATCCAGCTCCAGGTCTTCCTTAATGCCCCAGCATGCATTAGGCTGTGACATCACACC AACCACAAAACCCCAGTGGAGTATGGAACTGTCCCGAAAGGGAGCAGGTAAAGAGCAGCCTTTGGAGATG GGTGTGCATCCCGTGGCGGCCCCGCTCTCCGTGTTTGCTAAGGAGGCTCTGTCCTCTGCCCGGCACAGCG AGCACCACCACCACCATCACCATGAGCACAAGAAGAAGAAGAAGAAGCACAAACACAAGCACAAGCACAA GCACAAGCATGACGGCAAGGACAAGGACAAGGACAAGGAGCCCTTCACCTTCTCCAGCCCTGCCAGTGGC AGGTCTGTGCGCTCCCCTTCACTCTCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209940 representing NM_133319 Red=Cloning site Green=Tags(s) MPPTGLESSRMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQC RIYRVRINDLEAAFIYNDPTLEVCHSESKQRNLNYFSNAYAAAVSAVDPDAGNGELCIKVPSELWKHVDE LKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFWFPCVDSYSELCTWKLEFTVDA AMVAVSNGDLVETVYTHDMRKKTFHYVLAIPTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHT TSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSL AQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYHHWIKEELDKIVAYELKTGGVLLHPIFGG GKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQS HMWSQMLVSTYGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKY VGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRI DPDMSVLRKVEFEQADFMWQYELRYERDVVAQQESILALEKFPTPASRLALTDILEQEQCFYRVRMSACF CLAKIANSMVSTWTGPPAMKSLFTRMFCCKTCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHNLCPKE VLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNME KLLPSYRHTITVSCLRAIRVLQKNGHVPSDASLFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWL LNMIQTDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTAHDWRLRCGAVDLYFTLFG LSRPSCLPLPELGLVLNLKEKKAVLNPTIIPEAGVGNQETASNPGCHTQLAGFQNPFSSSQDEEEVDMDT VHDSQAFISHHLNMLERPSTPGLSKYRPSSSRSSLMPQHALGCDITPTTKPQWSMELSRKGAGKEQPLEM GVHPVAAPLSVFAKEALSSARHSEHHHHHHHEHKKKKKKHKHKHKHKHKHDGKDKDKDKEPFTFSSPASG RSVRSPSLSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133319
ORF Size	3600 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133319.1, NP_579853.1
RefSeq Size	5042 bp
RefSeq ORF	3603 bp
Locus ID	170844
MW	137.1 kDa
Gene Summary	mRNA expression increases in response to paraquat intake; may play a role in response to paraquat induced reactive oxygen species production and lung injury [RGD, Feb 2006]

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