Taf2 (NM_133319) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	TAFIIB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209940 representing NM_133319 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCCCACCGGCTTAGAGTCCTCCAGGATGAACAGGAAGAAAGGAGACAAGGGCTTCGAAAGCCCAC GGCCGTACAAATTAACCCATCAGGTCGTCTGCATCAACAACATAAACTTCCAGAGAAAGTCTGTTGTGGG GTTTGTGGAGCTGACTATATTTCCTACGGTTGCAAACTTGAATAGAATCAAGCTGAACAGTAAACAGTGT AGAATATACCGAGTCAGGATCAATGACTTGGAGGCTGCTTTTATTTATAATGATCCAACCTTAGAAGTTT GTCACAGCGAATCAAAACAGAGAAACCTCAATTATTTTTCCAATGCGTATGCAGCTGCAGTCAGTGCTGT GGACCCTGATGCGGGGAACGGAGAACTCTGCATTAAGGTCCCCTCAGAGCTCTGGAAGCACGTCGATGAG TTAAAGGTCCTGAAGATACATATTAATTTTTCTTTGGATCAGCCAAAAGGAGGTCTTCATTTTGTGGTAC CCAGTGTAGAGGGGAGTATGGCAGAGAGAGGTGCTCATGTCTTCTCTTGTGGGTATCAGAATTCTACAAG ATTTTGGTTCCCTTGTGTTGATTCATACTCTGAGTTGTGTACCTGGAAATTAGAATTTACAGTCGATGCT GCGATGGTGGCTGTGTCCAATGGAGATTTGGTGGAGACAGTGTATACTCACGACATGAGAAAGAAGACCT TCCATTATGTGCTTGCTATTCCTACAGCAGCGTCCAATATCTCCTTGGCCATCGGGCCATTTGAAATACT TGTAGATCCATATATGCACGAGGTTACCCATTTTTGTTTGCCTCAACTTCTTCCATTACTTAAACATACT ACATCATACCTTCACGAAGTTTTTGAATTTTATGAGGAAATTTTGACTTGTCGCTATCCGTACTCCTGTT TTAAGACGGTCTTCATTGATGAGGCTTACGTGGAAGTGGCCGCTTATGCGTCTATGAGCATTTTCAGCAC AAATCTCCTGCACAGCGCCATGATTATAGATGAGACACCGCTCACCAGGCGGTGCCTCGCACAGTCTCTA GCACAGCAGTTCTTCGGATGTTTCATCTCCAGAATGTCTTGGTCTGATGAGTGGGTGCTGAAGGGAATTT CAGGTTATATTTATGGACTGTGGATGAAGAAAACGTTTGGTGTCAATGAGTACCACCATTGGATTAAAGA GGAACTCGATAAAATAGTGGCATATGAACTGAAAACTGGGGGAGTTTTACTGCATCCCATATTTGGTGGT GGAAAAGAAAAGGATAACCCCGCATCTCACCTCCACTTTTCAATAAAGCATCCACATACACTGTCTTGGG AATACTACACTATGTTTCAGTGTAAAGCTCACCTCGTGATGAGACTGATTGAAAACAGAATCAGCATGGA ATTTATGCTGCAAGTTTTCAATAAACTATTAAGTCTGGCCAGTACCGCTTCATCCCAGAAGTTCCAGTCA CATATGTGGAGCCAGATGCTGGTCTCCACCTACGGGTTTTTGAAGTCCATTTCCAATGTCTCTGGCAAAG ACATCCAGCCCTTAATAAAGCAGTGGGTAGACCAGAGTGGAGTGGTAAAGTTTTATGGAAGTTTTGCATT TAATAGAAAACGAAATGTTTTAGAACTAGAAATAAAACAAGATTACACATCTCCTGGCACCCAGAAGTAC GTGGGACCACTTAAAGTGACAGTGCAAGAGTTAGACGGCTCCTTCAACCATACCTTGCAAATTGAAGAAA ACAGCCTTAAACATGACATACCCTGCCACTCCAAAAGCAGAAGGAATAAGAAGAAGAAAATCCCACTGAT GAATGGGGAAGAAGTCGATATGGATCTTTCTGCAATGGATGCGGACTCCCCTTTGCTGTGGATAAGGATA GACCCAGATATGTCTGTCCTGAGGAAGGTGGAGTTTGAGCAGGCTGACTTTATGTGGCAGTATGAGCTCC GCTATGAGAGAGATGTTGTTGCTCAGCAGGAATCCATCTTGGCCTTGGAAAAGTTCCCCACGCCCGCATC GCGCCTCGCGCTCACTGATATACTAGAGCAAGAGCAGTGTTTCTACCGAGTGAGGATGTCAGCATGCTTC TGCCTTGCAAAGATTGCAAACTCAATGGTGAGCACATGGACGGGACCACCAGCCATGAAGTCCCTCTTTA CTAGAATGTTTTGTTGTAAAACTTGTCCCAACATCGTGAAAACAAACAACTTCATGAGCTTTCAGAGTTA TTTTCTACAGAAGACTATGCCAGTTGCAATGGCTTTGTTAAGAGACGTACATAATCTTTGTCCCAAAGAA GTTTTAACATTTATTTTAGACTTAATCAAGTACAATGACAACAGAAAAAATAAGTTTTCAGATAACTATT ACCGTGCAGAAATGATTGATGCCTTGGCTAACTCTGTCACACCTGCTGTCAGCGTGAATAATGAAGTCAG AACTTTGGATAACTTAAATCCTGATGTGCGACTAATACTTGAAGAAATTACCCGGTTTTTGAATATGGAA AAACTTCTCCCAAGTTACAGACATACTATCACTGTCAGTTGTTTGAGAGCCATAAGAGTGCTTCAGAAGA ATGGACATGTGCCAAGTGATGCATCTCTTTTCAAATCCTATGCTGAGTATGGCCACTTCGTGGACATCAG GATAGCGGCTCTGGAAGCCGTCGTCGATTACACCAAAGTGGACAGGAGTTATGAAGAACTTCAATGGCTA CTTAATATGATTCAGACTGACCCTGTCCCCTATGTGAGGCATAAGATTCTAAACATGCTGACTAAAAATC CGCCATTTACAAAGAACATGGAGTCTCCTTTATGCAACGAGGCCTTGGTAGATCAGCTCTGGAAGCTGAT GAATTCTGGTACTGCACATGACTGGAGGTTACGGTGTGGAGCTGTGGACTTGTACTTCACGCTCTTTGGC CTCAGTAGACCTTCTTGTTTACCCTTGCCAGAGCTCGGGCTTGTTCTTAACCTAAAAGAGAAGAAAGCTG TCTTGAATCCAACCATAATTCCAGAAGCGGGAGTAGGCAATCAGGAAACTGCAAGTAACCCAGGCTGTCA CACTCAGCTAGCAGGATTTCAGAACCCTTTCTCAAGTTCTCAGGATGAGGAGGAGGTCGATATGGACACC GTTCATGATAGTCAGGCCTTCATTTCCCATCATCTGAACATGCTTGAAAGGCCGTCCACTCCAGGGCTCT CTAAGTACCGACCATCCAGCTCCAGGTCTTCCTTAATGCCCCAGCATGCATTAGGCTGTGACATCACACC AACCACAAAACCCCAGTGGAGTATGGAACTGTCCCGAAAGGGAGCAGGTAAAGAGCAGCCTTTGGAGATG GGTGTGCATCCCGTGGCGGCCCCGCTCTCCGTGTTTGCTAAGGAGGCTCTGTCCTCTGCCCGGCACAGCG AGCACCACCACCACCATCACCATGAGCACAAGAAGAAGAAGAAGAAGCACAAACACAAGCACAAGCACAA GCACAAGCATGACGGCAAGGACAAGGACAAGGACAAGGAGCCCTTCACCTTCTCCAGCCCTGCCAGTGGC AGGTCTGTGCGCTCCCCTTCACTCTCAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133319
Insert Size	3603 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133319.1, NP_579853.1
RefSeq Size	5042 bp
RefSeq ORF	3603 bp
Locus ID	170844
Gene Summary	mRNA expression increases in response to paraquat intake; may play a role in response to paraquat induced reactive oxygen species production and lung injury [RGD, Feb 2006]

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