Tnik (NM_001106422) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1561817
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209270 representing NM_001106422 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGACTCCCCAGCTCGCAGCCTGGATGAAATCGATCTCTCCGCCCTGAGGGACCCTGCAGGGA TCTTTGAGTTGGTGGAACTTGTCGGAAATGGTACATATGGCCAAGTTTATAAGGGTCGTCATGTCAAAAC AGGCCAGCTTGCTGCCATTAAGGTTATGGACGTCACAGGGGATGAAGAGGAAGAAATCAAACAAGAAATT AACATGTTGAAGAAATATTCTCATCACAGGAACATTGCTACATACTACGGTGCTTTTATCAAAAAGAACC CTCCGGGCATGGATGACCAACTATGGTTGGTTATGGAGTTCTGTGGTGCTGGCTCTGTCACTGACCTGAT CAAGAACACGAAAGGCAACACATTGAAAGAGGAGTGGATTGCATACATCTGCAGGGAGATCTTACGGGGC CTGAGTCACCTGCACCAGCACAAAGTGATTCATCGGGATATTAAAGGACAGAATGTCTTGCTGACTGAAA ATGCAGAAGTTAAGCTAGTGGATTTTGGCGTGAGTGCCCAGCTTGACCGAACAGTGGGCAGGAGGAACAC GTTCATCGGGACCCCCTACTGGATGGCACCCGAAGTCATTGCCTGTGATGAGAACCCAGATGCCACGTAT GATTTCAAGAGTGACTTGTGGTCTTTGGGAATCACCGCCATTGAGATGGCAGAAGGTGCCCCCCCCCTCT GTGATATGCATCCCATGAGAGCGCTCTTCCTCATCCCACGGAACCCCGCACCTCGCCTCAAGTCTAAGAA GTGGTCAAAAAAATTCCAGTCATTTATCGAGAGCTGCTTGGTAAAGAATCACGGCCAGCGGCCAGCCACA GAACAGTTGATGAAGCACCCATTCATACGAGACCAGCCTAATGAGAGGCAGGTCCGCATCCAGCTGAAGG ACCACATTGATCGAACAAAGAAGAAGCGAGGAGAAAAAGATGAGACTGAGTATGAATACAGTGGAAGTGA GGAAGAAGAGGAAGAGAACGACTCTGGGGAACCCAGCTCCATTCTGAACCTACCAGGAGAGTCAACACTG CGACGGGACTTCCTGAGACTGCAGCTGGCCAACAAGGAGCGCTCAGAGGCCCTGCGGCGCCAACAGCTGG AGCAGCAGCAGAGGGAGAATGAAGAACATAAGAGGCAGCTACTGGCTGAGCGCCAGAAGCGCATCGAGGA GCAGAAGGAGCAGAGGCGGAGGCTGGAGGAGCAACAAAGGCGAGAAAAGGAGCTTCGGAAGCAGCAGGAG CGGGAACAGCGCCGGCACTATGAGGAGCAGATGCGCCGGGAGGAGGAGAGGAGGCGCGCAGAACACGAAC AGGAGTACATCAGGCGACAGCTAGAAGAAGAGCAAAGGCAGTTAGAAATCTTACAGCAGCAGCTACTGCA TGAGCAAGCTCTACTTCTGGAATATAAGCGCAAACAACTGGAAGAACAGAGACAAGCAGAAAGACTGCAG CGGCAGCTAAAGCAAGAGCGGGACTATCTGGTTTCCCTCCAGCACCAGCGGCAGGAGCAGAGGCCCCTGG AGAAGAAGCCATTGTACCATTACAAGGAGGGCATGAGTCCTAGTGAGAAGCCTGCCTGGGCCAAGGAGGT GGAAGAACGCTCAAGACTCAACCGACAGAGTTCACCTGCCATGCCTCACAAGGTTGCCAACAGGATCTCA GACCCCAACCTACCCCCAAGATCAGAGTCCTTCAGCATTAGTGGGGTTCAGCCTGCTCGGACACCCCCAA TGCTCAGACCTGTTGATCCCCAGATCCCGCAGCTGGTAGCCGTCAAATCCCAGGGACCTGCCTTGACCGC CTCCCAGTCAGTGCATGAGCAACCCACAAAAGGCCTGTCTGGGTTCCAGGAGGCTCTGAATGTGACCTCT CACCGGGTCGAGATGCCACGCCAGAACTCGGATCCCACCTCGGAAAACCCTCCTCTCCCCACGCGCATTG AGAAGTTTGACCGAAGCTCTTGGTTACGACAGGAAGAAGACATTCCACCAAAGGTGCCTCAAAGAACAAC TTCTATATCCCCAGCACTAGCCAGAAAGAATTCCCCTGGGAATGGCAGTGCTTTAGGCCCAAGACTCGGA TCTCAGCCTATCAGAGCAAGCAACCCTGATCTCCGCAGGACAGAGCCAGTCCTGGAGAGCTCCTTGCAGC GGACCAGCAGTGGCAGTTCTTCCAGCTCCAGCACTCCCAGCTCCCAGCCCAGCTCTCAAGGAGGCTCTCA GCCTGGGTCACAAGCAGGATCTAGTGAACGAACCAGAGTTCGGGCCAACAGTAAGTCAGAAGGATCGCCC GTGCTCCCCCATGAGCCTTCCAAGGTGAAACCAGAAGAATCCAGAGACATCCCACGGCCCAGCCGGCCAG CTGATCTGACGGCGTTAGCCAAAGAATTAAGAGAACTCCGGATTGAAGAAACAAACCGCCCACTGAAGAA AGTGACTGATTACTCTTCCTCCAGCGAGGAGTCAGAGAGCAGTGAGGAAGAAGAGGAGGATGGAGAGAGT GAGACGCACGATGGGACAGTGGCTGTCAGTGACATACCCAGACTAATACCCACGGGTGCTCCAGGGAAGA ATGAGCAGTACAACATGGGGATGGTCGGCACACATGGGCTGGAGACTTCGCATGCAGATACCTTTGGCGG CAGCATTTCAAGAGAAGGAACCTTGATGATCAGAGAGACGGCTGAAGAGAAGAAGCGATCTGGCCACAGT GACAGTAATGGCTTCGCAGGTCACATCAATCTCCCAGACCTTGTGCAGCAGAGCCATTCGCCAGCTGGGA CTCCCACTGAGGGCCTGGGCCGTGTCTCCACTCATTCCCAGGAGATGGACACTGGAGCTGAATATGGTAT GGGAAGCAGCACCAAAGCCTCCTTCACCCCCTTCGTGGACCCTAGAGTGTACCAGACATCGCCCACTGAT GAAGATGAAGAGGATGATGAGTCTTCGGCTGCTGCCCTGTTTACTAGCGAACTTCTTAGGCAAGAACAGG CCAAACTCAATGAAGCGAGGAAGATTTCAGTGGTAAATGTGAACCCAACGAACATTCGCCCTCACAGTGA CACACCGGAAATCAGAAAATACAAGAAACGATTCAATTCCGAAATACTCTGTGCAGCTCTGTGGGGTGTG AACCTTCTGGTGGGGACTGAAAATGGCCTGATGCTTTTGGACAGAAGCGGCCAAGGCAAAGTCTACAACC TCATCAACCGGAGGCGATTTCAGCAGATGGACGTGCTAGAAGGACTAAACGTTCTTGTCACGATATCAGG AAAGAAGAATAAGCTCCGGGTGTACTATCTCTCGTGGTTAAGAAACAGAATCCTGCACAATGACCCAGAA GTAGAAAAGAAGCAGGGTTGGATCACCGTCGGAGACTTGGAAGGCTGTATCCATTACAAAGTTGTTAAAT ATGAAAGGATCAAGTTCTTGGTGATTGCCTTAAAGAATGCAGTAGAGATATACGCATGGGCGCCTAAACC TTATCATAAGTTCATGGCATTTAAGTCTTTTGCAGATCTTCAGCATAAGCCTCTGCTCGTTGACCTCACA GTAGAAGAAGGTCAAAGGTTAAAGGTCATTTTTGGCTCACACACTGGTTTCCATGTAATTGATGTTGACT CTGGAAACTCCTACGATATCTATATACCATCCCATATTCAGGGTAATATCACTCCTCACGCTATCGTCAT CTTGCCTAAAACAGACGGAATGGAGATGCTTGTCTGCTATGAGGATGAGGGGGTGTATGTGAACACCTAT GGCCGGATCACTAAGGATGTGGTGCTCCAATGGGGAGAAATGCCCACATCTGTGGCCTACATTCATTCCA ATCAGATAATGGGCTGGGGCGAGAAAGCTATTGAGATCCGGTCAGTGGAAACAGGACATTTGGATGGAGT GTTTATGCATAAACGAGCTCAAAGGTTAAAGTTTCTATGTGAAAGAAATGATAAGGTAAATCCGTTTCAA CTTTGCTTTAGTGTTTGCATTTTAAGAGACCACCAGGTACCTGTGAAACCTTCATTTTCCTTTATACTGA CT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209270 representing NM_001106422 Red=Cloning site Green=Tags(s) MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEI NMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRG LSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHGQRPAT EQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTL RRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQE REQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQ RQLKQERDYLVSLQHQRQEQRPLEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRIS DPNLPPRSESFSISGVQPARTPPMLRPVDPQIPQLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTS HRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLG SQPIRASNPDLRRTEPVLESSLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSP VLPHEPSKVKPEESRDIPRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSEEEEEDGES ETHDGTVAVSDIPRLIPTGAPGKNEQYNMGMVGTHGLETSHADTFGGSISREGTLMIRETAEEKKRSGHS DSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDTGAEYGMGSSTKASFTPFVDPRVYQTSPTD EDEEDDESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGV NLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPE VEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLT VEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTY GRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVNPFQ LCFSVCILRDHQVPVKPSFSFILT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106422
ORF Size	4062 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106422.1, NP_001099892.1
RefSeq Size	4523 bp
RefSeq ORF	4065 bp
Locus ID	294917
MW	154.3 kDa

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