Abl2 (NM_001107186) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Abll; Arg
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206195 representing NM_001107186 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGCAGGTGGGCCGCGTCGGGGAGGCTCCCGGGCTCCAGCAGCCACAGCCGCGCGGGATCCGGG GCAGCAGCGCAGCCAGGCCCTCCGGCCGCAGGCGGGACCCGGTGGGGCGTACCGCAGACGCCGGCTTCAA CGTCTTCACCCAGCACGGACAGGGCGACTGTCGAACAGTGGATGGTATCCCCAGGAAGGGAAGGATGAAG GCAGAAGTTTTGCACCGCCCCTTTGGTTGTGACGCTGAATCTCAGGCACTGAATGAAGCTATCAGGTGGA GCTCCAAGGAGAACTTGCTGGGCGCCACTGAGAGCGACCCTAATCTCTTTGTTGCACTTTATGACTTTGT GGCAAGTGGCGATAACACACTCAGCATCACCAAAGGTGAAAAGCTACGAGTCCTTGGTTACAACCAAAAT GGTGAATGGAGTGAAGTTCGCTCCAAGAATGGGCAGGGTTGGGTGCCCAGCAACTACATCACTCCAGTCA ACAGCCTGGAGAAACATTCCTGGTACCATGGGCCTGTGTCCCGCAGTGCAGCGGAGTATCTCCTCAGCAG CCTTATCAATGGCAGTTTCCTGGTGCGAGAGAGTGAGAGCAGCCCTGGGCAGCTGTCCATCTCTCTCAGA TATGAGGGACGTGTGTATCACTACAGGATCAATACCACCACAGACAGCAAGGTGTACGTGACTGCCGAGA GCCGCTTCAGCACCTTAGCAGAGCTTGTTCACCACCACTCCACAGTCGCTGATGGGCTAGTGACCACGCT ACATTACCCAGCACCCAAGTGCAACAAGCCAACCGTCTACGGTGTGTCTCCTATCCACGACAAGTGGGAG ATGGAGCGCACAGATATCACCATGAAGCACAAGCTTGGGGGCGGCCAGTATGGAGAGGTTTACGTTGGCG TCTGGAAGAAATACAGCCTTACAGTTGCTGTGAAAACACTGAAGGAAGATACCATGGAGGTAGAGGAGTT CCTGAAGGAAGCTGCAGTGATGAAGGAAATCAAGCATCCTAATTTAGTACAGCTGTTAGGTGTGTGCACC CTGGAGCCGCCATTCTACATTGTGACTGAATACATGCCGTACGGGAATCTGCTCGATTACCTCCGGGAGT GCAGCCGTGAGGAGGTGACTGCAGTGGTGCTGCTTTACATGGCCACTCAGATCTCCTCTGCCATGGAGTA CTTGGAGAAGAAGAACTTCATCCACAGAGATCTTGCTGCACGGAACTGCCTGGTGGGGGAGAACCATGTG GTGAAAGTGGCTGACTTTGGTCTAAGTAGACTGATGACTGGAGACACCTACACCGCTCATGCTGGAGCCA AATTTCCTATTAAATGGACAGCGCCTGAGAGTCTGGCCTACAATACCTTTTCAATTAAATCTGACGTTTG GGCTTTTGGAGTACTGTTGTGGGAAATTGCTACGTATGGAATGTCACCATATCCAGGTATTGACCTATCT CAAGTCTATGACCTACTGGAAAAAGGATATCGAATGGAGCAGCCTGAGGGATGCCCCCCTAAAGTGTATG AACTTATGAGAGCATGCTGGAAGTGGAGCCCTGCGGACAGGCCCTCTTTTGCTGAAACCCATCAAGCTTT TGAAACCATGTTCCATGACTCCAGCATCTCTGAAGAGGTAGCTGAGGAGCTTGGGAGAACTGCCTCCTCC TCATCTGTAGTTCCATACCTGCCTCGACTGCCACTACTTCCTTCCAAGACAAGGACCCTGAAGAAGCAGG GGGAGAACAAAGAGAACCTAGATGGGGGCCTTGATGCCTCCGAGATCCTGGCCTCCAGCTCAGCACCAGG GTTCATTAGAAGCACCCAGGCCTCCAGTGGGTCCCCAGCTCTGCCTCGAAAGCAAAGAGATAAGTCACCC AGCAGCCTCTTAGAAGATGCCAAAGAGACATGCTTCACCAGGGACAGGAAGGGAGGCTTCTTCAGCTCCT TCATGAAAAAAAGAAATGCCCCCATGCCCCCTAAGCGCAGCAGCTCTTTTCGAGAAATGGAGAATCAGCC CCACAAGAAATATGAACTCACGGGTAACTTCTCACCTGTTGCTTCTCTGCAGCATGCTGATGGGTTCTCT TTCACTCCCGGCCAGCAAGAGTCGAATCTGGTGCCAGCCAAGTGCTATGGGGGGAGCTTTGCACAGAGGA ACCTCTGTGGTGATGATGACAGTGGCTTCTTTACACCTCGCTTAATCAAAAAGACACTGGGCTTGCGAGC AGGTAAACCGACAGCCAGTGATGACACTTCCAAGCCTTTTCCAAGGTCAAACTCTACATCTTCCATGTCC TCAGGGCTCCCCGAGCAGGATAGGATGGCAATGACCCTTCCCAGGAACTGCCAGAGGTCCAAACTCCAGC TGGAAAGGACAGTGTCCACCTCTTCTCAACCAGAAGAGAATGTGGACAGGGCTAATGACATGCTTCCAAA AAAATCAGAGGAAGGTGCTGCTCCAGCCAGGGAGAGACCAAAAGCCAAACTATTGCCCAGAGGAGCCACG GCTCTTGCCCTGAGAGCCCCTGATCCAGCCATCACTGAGAGTGACTGTCCAGGGGTAGGGGTGGCTGGAG TGGCAGCTGCCCCCAAAGGCAAGGAGAGGAATGGTGGGACGCGGCTTGGAGTCGCTGGAGTCCCAGAGGA CGCAGAGCAGCCAGGCTGGTCTTCTCCAGCCAAGGCTGTAGCTGTCCTCCCAACCACTCACAACCACAAA GTGCCAGTCCTTATCTCACCCACTCTGAAACACACTGCAGCTGATGTGCAGCTCATTGGCACAGACTCTC AGGGGAACAAATTCAAGCTCTTATCTGAGCATCAGGTCACATCCTCTGGAGACAAGGACCGACCCCGACG GGTAAAACCAAAGTGTGCCCCACCCCCGCCACCAGTGATGAGACTACTGCAGCATCCGTCCACATGCTCA GACCCCGAGGAAGAGCCAACTGCCCCCCCTGCAGGACAGCACACTCCAGACACCCAGGACGGAGGAAAAA AGGCAGCTCCAGGGCCAGTGCCCAGTAGTGGGAAACCTGGGAGGCCAGTGATGCCTCCACCTCAAGTGCC TTTGCCCACATCTTCCATCTCGCCAGCCAAAATGGCCAATGGCACAGCAGGTACTAAGGTGGCCCTGAGG AAAACCAAACAGGCAGCTGAGAAAATCTCAGCTGACAAAATCAGCAAAGCGGCCCTGCTGGAGTGTGCCG GCCTACTGTCCAGTGCAATCACAGAACCTGTGCCCCATAGCCAGCTGGTGGACACTGGGCACCAGCTACT CCACTACTGCTCAGGGTATGTGGACTGCATCCCCCACACTCGCAACAAATTTGCCTTCCGAGAGGCTGTG AGCAAACTGGAACTTAGCTTACAGGAGCTGCAGGTGTCTTCCACAGCTGCTGGTGTGCCTGGGACAAACC CTGTCCTTAATAACTTATTGTCATGTGTACAGGAAATTAGTGATGTGGTGCAGAGGGCCTGCAGGTCACT CGCTCTCCTCGGCCACCATGCGGGCCCGGTAGGGACTCAGCGAAAGCGCTCTCCTCGGCCACCAGGAGGG CCGCATAGGGACCCAGCAAAAGCGCACTGGCTCCCCGGGCCGCCGACACCAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206195 representing NM_001107186 Red=Cloning site Green=Tags(s) MGQQVGRVGEAPGLQQPQPRGIRGSSAARPSGRRRDPVGRTADAGFNVFTQHGQGDCRTVDGIPRKGRMK AEVLHRPFGCDAESQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQN GEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLR YEGRVYHYRINTTTDSKVYVTAESRFSTLAELVHHHSTVADGLVTTLHYPAPKCNKPTVYGVSPIHDKWE MERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCT LEPPFYIVTEYMPYGNLLDYLRECSREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHV VKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLS QVYDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVAEELGRTASS SSVVPYLPRLPLLPSKTRTLKKQGENKENLDGGLDASEILASSSAPGFIRSTQASSGSPALPRKQRDKSP SSLLEDAKETCFTRDRKGGFFSSFMKKRNAPMPPKRSSSFREMENQPHKKYELTGNFSPVASLQHADGFS FTPGQQESNLVPAKCYGGSFAQRNLCGDDDSGFFTPRLIKKTLGLRAGKPTASDDTSKPFPRSNSTSSMS SGLPEQDRMAMTLPRNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEEGAAPARERPKAKLLPRGAT ALALRAPDPAITESDCPGVGVAGVAAAPKGKERNGGTRLGVAGVPEDAEQPGWSSPAKAVAVLPTTHNHK VPVLISPTLKHTAADVQLIGTDSQGNKFKLLSEHQVTSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSTCS DPEEEPTAPPAGQHTPDTQDGGKKAAPGPVPSSGKPGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALR KTKQAAEKISADKISKAALLECAGLLSSAITEPVPHSQLVDTGHQLLHYCSGYVDCIPHTRNKFAFREAV SKLELSLQELQVSSTAAGVPGTNPVLNNLLSCVQEISDVVQRACRSLALLGHHAGPVGTQRKRSPRPPGG PHRDPAKAHWLPGPPTPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107186
ORF Size	3624 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107186.1, NP_001100656.1
RefSeq Size	3764 bp
RefSeq ORF	3627 bp
Locus ID	304883
MW	131.5 kDa

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