United States
Germany
Japan
United Kingdom
China
DNA Ligase I (LIG1) (NM_001289063) Human Tagged ORF Clone
CAT＃: RG239739
- TrueORF®
LIG1 (tGFP-tagged) - Human ligase I, DNA, ATP-dependent (LIG1), transcript variant 2
ORF Plasmid: DDK
"NM_001289063" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 7,510.00
货期*
2周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239739 representing NM_001289063.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGCGAAGTATCATGGCGGCACTGAAGGAGTGGAATGGAGTGGTGTCCGAGAGTGACTCTCCGGTG AAGAGGCCAGGGAGGAAGGCGGCCCGGGTCCTGGGCAGCGAAGGGGAAGAGGAGGATGAAGCCCTTAGC CCTGCTAAAGGCCAGAAGCCTGCCCTGGACTGCTCACAGGTCTCCCCGCCCCGTCCTGCCACATCTCCT GAGAACAATGCTTCCCTCTCTGACACCTCTCCCATGGACAGTTCCCCATCAGGGATTCCGAAGCGTCGC ACAGCTCGGAAGCAGCTCCCGAAACGGACCATTCAGGAAGTCCTGGAAGAGCAGAGTGAGGACGAGGAC AGAGAAGCCAAGAGGAAGAAGGAGGAGGAAGAGGAGACCCCGAAAGAAAGCCTCACAGAGGCTGAAGTG GCCACAGAGAAGGAAGGAGAAGACGGGGACCAGCCCACCACGCCTCCCAAGCCCCTAAAGACCTCCAAA GCAGAGACCCCGACGGAAAGCGTTTCAGAGCCTGAGGTGGCCACGAAGCAGGAACTGCAGGAGGAGGAA GAGCAGACCAAGCCTCCCCGCAGAGCTCCCAAGACGCTCAGCAGCTTCTTCACCCCCCGGAAGCCAGCA GTCAAAAAAGAAGTGAAGGAAGAGGAGCCAGGGGCTCCAGGAAAGGAGGGAGCTGCTGAGGGACCCCTG GATCCATCTGGTTACAATCCTGCCAAGAACAACTATCATCCCGTGGAAGATGCCTGCTGGAAACCGGGC CAGAAGGTTCCTTACCTGGCTGTGGCCCGGACGTTTGAGAAGATCGAGGAGGTGTCTGCTCGGCTCCGG ATGGTGGAGACGCTGAGCAACTTGCTGCGCTCCGTGGTGGCCCTGTCGCCTCCAGACCTCCTCCCTGTC CTCTACCTCAGCCTCAACCACCTTGGGCCACCCCAGCAGGGCCTGGAGCTTGGCGTGGGTGATGGTGTC CTTCTCAAGGCAGTGGCCCAGGCCACAGGTCGGCAGCTGGAGTCCGTCCGGGCTGAGGCAGCCGAGAAA GGCGACGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACCCAGAGGCTCATGCTGCCACCACCTCCGCTC ACTGCCTCCGGGGTCTTCAGCAAGTTCCGCGACATCGCCAGGCTCACTGGCAGTGCTTCCACAGCCAAG AAGATAGACATCATCAAAGGCCTCTTTGTGGCCTGCCGCCACTCAGAAGCCCGGTTCATCGCTAGGTCC CTGAGCGGACGGCTGCGCCTTGGGCTGGCAGAGCAGTCGGTGCTGGCTGCCCTCTCCCAGGCAGTGAGC CTCACGCCCCCGGGCCAAGAATTCCCACCAGCCATGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCC AGAAAGACGTGGCTGGAGGAGCAAGGCATGATCCTGAAGCAGACGTTCTGCGAGGTTCCCGACCTGGAC CGAATTATCCCCGTGCTGCTGGAGCACGGCCTGGAACGTCTCCCGGAGCACTGCAAGCTGAGCCCAGGG ATTCCCCTGAAACCAATGTTGGCCCATCCCACCCGGGGCATCAGCGAGGTCCTGAAACGCTTTGAGGAG GCAGCTTTCACCTGCGAATACAAATATGACGGGCAGAGGGCACAGATCCACGCCCTGGAAGGCGGGGAG GTGAAGATCTTCAGCAGGAATCAGGAAGACAACACTGGGAAGTACCCGGACATCATCAGCCGCATCCCC AAGATTAAACTCCCATCGGTCACATCCTTCATCCTGGACACCGAAGCCGTGGCTTGGGACCGGGAAAAG AAGCAGATCCAGCCATTCCAAGTGCTCACCACCCGCAAACGCAAGGAGGTGGATGCGTCTGAGATCCAG GTGCAGGTGTGTTTGTACGCCTTCGACCTCATCTACCTCAATGGAGAGTCCCTGGTACGTGAGCCCCTT TCCCGGCGCCGGCAGCTGCTCCGGGAGAACTTTGTGGAGACAGAGGGCGAGTTTGTCTTCGCCACCTCC CTGGACACCAAGGACATCGAGCAGATCGCCGAGTTCCTGGAGCAGTCAGTGAAAGACTCCTGCGAGGGG CTGATGGTGAAGACCCTGGATGTTGATGCCACCTACGAGATCGCCAAGAGATCGCACAACTGGCTCAAG CTGAAGAAGGACTACCTTGATGGCGTGGGTGACACCCTGGACCTGGTGGTGATCGGCGCCTACCTGGGC CGGGGGAAGCGGGCCGGCCGGTACGGGGGCTTCCTGCTGGCCTCCTACGACGAGGACAGTGAGGAGCTG CAGGCCATATGCAAGCTTGGAACTGGCTTCAGTGATGAGGAGCTGGAGGAGCATCACCAGAGCCTCAAG GCGCTGGTGCTGCCCAGCCCACGCCCTTACGTGCGGATAGATGGCGCTGTGATTCCCGACCACTGGCTG GACCCCAGCGCTGTGTGGGAGGTGAAGTGCGCTGACCTCTCCCTCTCTCCCATCTACCCTGCTGCGCGG GGCCTGGTGGATAGTGACAAGGGCATCTCCCTTCGCTTCCCTCGGTTTATTCGAGTCCGTGAAGACAAG CAGCCGGAGCAGGCCACCACCAGTGCTCAGGTGGCCTGTTTGTACCGGAAGCAAAGTCAGATTCAGAAC CAACAAGGCGAGGACTCAGGCTCTGACCCTGAAGATACCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239739
Blue=ORF Red=Cloning site Green=Tag(s) MQRSIMAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSP ENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEETPKESLTEAEV ATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPA VKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLR MVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEK GDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARS LSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLD RIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGE VKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQ VQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEG LMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEEL QAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAAR GLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001289063 |
| ORF Size | 2664 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001289063.2 |
| RefSeq Size | 3317 bp |
| RefSeq ORF | 2667 bp |
| Locus ID | 3978 |
| UniProt ID | P18858 |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair |
| MW | 98.7 kDa |
| Gene Summary | This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
其它DNA Ligase I产品
Customer
Reviews
Loading...
