United States
Germany
Japan
United Kingdom
China
THBS3 (NM_001252607) Human Tagged ORF Clone
CAT＃: RG235072
- TrueORF®
THBS3 (tGFP-tagged) - Homo sapiens thrombospondin 3 (THBS3), transcript variant 2
ORF Plasmid: DDK
"NM_001252607" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 7,890.00
货期*
5周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TSP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235072 representing NM_001252607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGCAGGAACTTCGGGGGGCCCTGGCTCTTCTCCTCCTTTGCTTTTTCACATCTGCCAGTCAGG ATCTGCAGGTAATTGACCTGCTGACTGTGGGCGAGTCTCGGCAGATGGTAGCTGTGGCAGAGAAGATCCG GACAGCCTTGCTCACTGCTGGGGACATCTACCTCTTATCCACCTTCCGCCTGCCCCCCAAGCAGGGTGGT GTCCTCTTTGGCCTCTATTCTCGCCAAGACAACACTCGATGGCTGGAGGCCTCTGTTGTAGGCAAGATCA ACAAAGTACTGGTGCGATACCAGCGGGAGGATGGCAAAGTCCACGCCGTGAACCTACAGCAAGCGGGCCT GGCTGATGGGCGCACACACACAGTTCTCCTGCGACTCCGAGGTCCCTCCAGACCCAGCCCTGCCCTACAT CTCTACGTGGACTGCAAACTGGGTGACCAACATGCAGGCCTTCCAGCACTGGCCCCCATTCCTCCAGCGG AGGTCGATGGGCTGGAGATTAGGACTGGACAGAAGGCGTATTTGAGGATGCAGGGCTTTGTGGAATCTAT GAAAATTATTCTGGGTGGGTCCATGGCCCGGGTAGGAGCCCTGAGTGAGTGTCCATTCCAAGGGGACGAG TCCATCCACAGTGCAGGGGAGCAGACCAAGGCGCTGGTCACCCAACTCACCCTCTTCAACCAGATCCTGG TGGAGCTGCGGGATGATATACGAGACCAGGTGAAGGAAATGTCCCTGATCCGAAACACCATTATGGAGTG TCAGGTGTGCGGCTTCCATGAGCAGCGTTCCCACTGCAGCCCCAATCCCTGCTTCCGAGGTGTGGACTGC ATGGAAGTGTACGAGTACCCAGGCTACCGCTGTGGGCCCTGCCCCCCTGGCCTGCAGGGCAACGGCACCC ACTGCAGTGACATCAATGAGTGTGCTCACGCTGACCCCTGTTTCCCGGGCTCCAGCTGCATCAACACCAT GCCCGGCTTCCACTGTGAGGCCTGTCCTCGAGGGTACAAGGGCACACAGGTGTCTGGTGTGGGCATTGAC TATGCCCGGGCCAGCAAACAGGTCTGCAATGACATCGATGAATGCAACGATGGCAACAATGGTGGCTGTG ACCCAAACTCCATCTGCACCAACACTGTGGGCTCTTTCAAGTGTGGTCCCTGCCGCCTGGGTTTCCTGGG CAACCAGAGCCAGGGCTGCCTCCCAGCCCGGACCTGCCACAGCCCAGCCCACAGCCCCTGCCACATCCAT GCTCACTGTCTCTTTGAACGCAATGGTGCAGTGTCCTGCCAGTGTAACGTGGGCTGGGCTGGGAATGGGA ACGTGTGTGGGACTGACACAGACATCGATGGCTACCCAGACCAAGCACTGCCCTGCATGGACAACAACAA ACACTGCAAACAGGACAACTGCCTTTTGACACCCAACTCTGGGCAGGAAGATGCTGATAATGATGGTGTG GGGGACCAGTGTGATGATGATGCTGATGGGGATGGGATCAAGAATGTTGAGGACAACTGCCGGCTGTTCC CCAACAAAGACCAGCAGAACTCAGATACAGATTCATTTGGTGATGCCTGTGACAATTGCCCCAACGTTCC CAACAATGACCAGAAGGACACAGATGGCAATGGGGAAGGAGATGCCTGTGACAACGACGTGGATGGGGAT GGCATCCCCAATGGATTGGACAATTGCCCTAAAGTCCCCAACCCACTACAGACAGACAGGGATGAGGACG GGGTGGGAGATGCTTGCGACAGCTGCCCTGAAATGAGCAATCCTACCCAGACAGATGCAGACAGCGACCT GGTGGGGGATGTCTGTGATACTAATGAAGACAGCGATGGGGATGGGCATCAGGACACCAAGGACAACTGC CCACAGCTGCCAAATAGCTCCCAGCTGGACTCTGATAACGATGGACTTGGAGATGAGTGTGATGGGGATG ATGACAATGATGGCATCCCAGATTATGTGCCTCCTGGTCCCGATAACTGCCGCCTGGTACCCAATCCCAA TCAGAAGGACTCAGATGGCAATGGCGTTGGTGATGTGTGTGAGGATGACTTTGACAATGATGCTGTGGTC GACCCCCTGGATGTGTGTCCTGAAAGTGCAGAGGTAACGCTTACGGATTTTCGGGCCTATCAGACCGTCG TCCTGGATCCTGAGGGTGATGCTCAGATTGACCCAAACTGGGTTGTGCTCAACCAGGGCATGGAAATCGT TCAGACCATGAACAGTGACCCTGGCTTGGCAGTTGGATACACGGCCTTCAATGGTGTGGACTTTGAAGGC ACCTTCCATGTGAACACAGTGACTGATGATGACTACGCAGGCTTTCTCTTCAGTTATCAAGACAGTGGCC GCTTCTACGTAGTCATGTGGAAGCAGACCGAGCAGACCTACTGGCAGGCTACACCCTTCCGGGCGGTTGC CCAGCCCGGGCTGCAGCTCAAGGCAGTGACATCAGTGTCTGGCCCAGGTGAGCACCTCCGAAATGCCCTG TGGCATACTGGCCACACCCCTGATCAGGTACGACTGCTGTGGACAGACCCACGAAATGTGGGCTGGCGGG ACAAGACCTCCTATCGCTGGCAGCTTCTGCACCGGCCTCAAGTTGGCTACATTCGGGTGAAGCTCTATGA GGGACCCCAGCTTGTGGCGGATTCTGGGGTGATCATTGACACATCCATGCGAGGGGGGCGTCTTGGTGTA TTCTGCTTCTCCCAAGAAAACATAATTTGGTCCAATCTCCAGTATCGATGCAATGACACAGTGCCTGAGG ACTTTGAGCCATTCCGGAGGCAGCTGCTCCAGGGAAGGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235072 representing NM_001252607
Red=Cloning site Green=Tags(s) METQELRGALALLLLCFFTSASQDLQVIDLLTVGESRQMVAVAEKIRTALLTAGDIYLLSTFRLPPKQGG VLFGLYSRQDNTRWLEASVVGKINKVLVRYQREDGKVHAVNLQQAGLADGRTHTVLLRLRGPSRPSPALH LYVDCKLGDQHAGLPALAPIPPAEVDGLEIRTGQKAYLRMQGFVESMKIILGGSMARVGALSECPFQGDE SIHSAGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIRNTIMECQVCGFHEQRSHCSPNPCFRGVDC MEVYEYPGYRCGPCPPGLQGNGTHCSDINECAHADPCFPGSSCINTMPGFHCEACPRGYKGTQVSGVGID YARASKQVCNDIDECNDGNNGGCDPNSICTNTVGSFKCGPCRLGFLGNQSQGCLPARTCHSPAHSPCHIH AHCLFERNGAVSCQCNVGWAGNGNVCGTDTDIDGYPDQALPCMDNNKHCKQDNCLLTPNSGQEDADNDGV GDQCDDDADGDGIKNVEDNCRLFPNKDQQNSDTDSFGDACDNCPNVPNNDQKDTDGNGEGDACDNDVDGD GIPNGLDNCPKVPNPLQTDRDEDGVGDACDSCPEMSNPTQTDADSDLVGDVCDTNEDSDGDGHQDTKDNC PQLPNSSQLDSDNDGLGDECDGDDDNDGIPDYVPPGPDNCRLVPNPNQKDSDGNGVGDVCEDDFDNDAVV DPLDVCPESAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEG TFHVNTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSVSGPGEHLRNAL WHTGHTPDQVRLLWTDPRNVGWRDKTSYRWQLLHRPQVGYIRVKLYEGPQLVADSGVIIDTSMRGGRLGV FCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001252607 |
| ORF Size | 2841 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001252607.1, NP_001239536.1 |
| RefSeq Size | 3224 bp |
| RefSeq ORF | 2844 bp |
| Locus ID | 7059 |
| UniProt ID | P49746 |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
