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PTPRN (NM_001199764) Human Tagged ORF Clone
CAT＃: RG234994
- TrueORF®
PTPRN (tGFP-tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, N (PTPRN), transcript variant 3
ORF Plasmid: DDK
"NM_001199764" in other vectors (2)
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CNY 7,890.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IA-2; IA-2/PTP; IA2; ICA512; R-PTP-N |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234994 representing NM_001199764
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAAGGATTGTCCTGGCACGATGACCTCACCCAGTATGTGATCTCTCAGGAGATGGAGCGCATCC CCAGGCTTCGCCCCCCAGAGCCCCGTCCAAGGGACAGGTCTGGCTTGGCACCCAAGAGACCTGGTCCTGC TGGAGAGCTGCTTTTACAGGACATCCCCACTGGCTCCGCCCCTGCTGCCCAGCATCGGCTTCCACAACCA CCAGTGGGCAAAGGTGGAGCTGGGGCCAGCTCCTCTCTGTCCCCTCTGCAGGCTGAGCTGCTCCCGCCTC TCTTGGAGCACCTGCTGCTGCCCCCACAGCCTCCCCACCCTTCACTGAGTTACGAACCTGCCTTGCTGCA GCCCTACCTGTTCCACCAGTTTGGCTCCCGTGATGGCTCCAGGGTCTCAGAGGGCTCCCCAGGGATGGTC AGTGTCGGCCCCCTGCCCAAGGCTGAAGCCCCTGCCCTCTTCAGCAGAACTGCCTCCAAGGGCATATTTG GGGACCACCCTGGCCACTCCTACGGGGACCTTCCAGGGCCTTCACCTGCCCAGCTTTTTCAAGACTCTGG GCTGCTCTATCTGGCCCAGGAGTTGCCAGCACCCAGCAGGGCCAGGGTGCCAAGGCTGCCAGAGCAAGGG AGCAGCAGCCGGGCAGAGGACTCCCCAGAGGGCTATGAGAAGGAAGGACTAGGGGATCGTGGAGAGAAGC CTGCTTCCCCAGCTGTGCAGCCAGATGCGGCTCTGCAGAGGCTGGCCGCTGTGCTGGCGGGCTATGGGGT AGAGCTGCGTCAGCTGACCCCTGAGCAGCTCTCCACACTCCTGACCCTGCTGCAGCTACTGCCCAAGGGT GCAGGAAGAAATCCGGGAGGGGTTGTAAATGTTGGAGCTGATATCAAGAAAACAATGGAGGGGCCGGTGG AGGGCAGAGACACAGCAGAGCTTCCAGCCCGCACATCCCCCATGCCTGGACACCCCACTGCCAGCCCTAC CTCCAGTGAAGTCCAGCAGGTGCCAAGCCCTGTCTCCTCTGAGCCTCCCAAAGCTGCCAGACCCCCTGTG ACACCTGTCCTGCTAGAGAAGAAAAGCCCACTGGGCCAGAGCCAGCCCACGGTGGCAGGACAGCCCTCAG CCCGCCCAGCAGCAGAGGAATATGGCTACATCGTCACTGATCAGAAGCCCCTGAGCCTGGCTGCAGGAGT GAAGCTGCTGGAGATCCTGGCTGAGCATGTGCACATGTCCTCAGGCAGCTTCATCAACATCAGTGTGGTG GGACCAGCCCTCACCTTCCGCATCCGGCACAATGAGCAGAACCTGTCTTTGGCTGATGTGACCCAACAAG CAGGGCTGGTGAAGTCTGAACTGGAAGCACAGACAGGGCTCCAAATCTTGCAGACAGGAGTGGGACAGAG GGAGGAGGCAGCTGCAGTCCTTCCCCAAACTGCGCACAGCACCTCACCCATGCGCTCAGTGCTGCTCACT CTGGTGGCCCTGGCAGGTGTGGCTGGGCTGCTGGTGGCTCTGGCTGTGGCTCTGTGTGTGCGGCAGCATG CGCGGCAGCAAGACAAGGAGCGCCTGGCAGCCCTGGGGCCTGAGGGGGCCCATGGTGACACTACCTTTGA GTACCAGGACCTGTGCCGCCAGCACATGGCCACGAAGTCCTTGTTCAACCGGGCAGAGGGTCCACCGGAG CCTTCACGGGTGAGCAGTGTGTCCTCCCAGTTCAGCGACGCAGCCCAGGCCAGCCCCAGCTCCCACAGCA GCACCCCGTCCTGGTGCGAGGAGCCGGCCCAAGCCAACATGGACATCTCCACGGGACACATGATTCTGGC ATACATGGAGGATCACCTGCGGAACCGGGACCGCCTTGCCAAGGAGTGGCAGGCCCTCTGTGCCTACCAA GCAGAGCCAAACACCTGTGCCACCGCGCAGGGGGAGGGCAACATCAAAAAGAACCGGCATCCTGACTTCC TGCCCTATGACCATGCCCGCATAAAACTGAAGGTGGAGAGCAGCCCTTCTCGGAGCGATTACATCAACGC CAGCCCCATTATTGAGCATGACCCTCGGATGCCAGCCTACATAGCCACGCAGGGCCCGCTGTCCCATACC ATCGCAGACTTCTGGCAGATGGTGTGGGAGAGCGGCTGCACCGTCATCGTCATGCTGACCCCGCTGGTGG AGGATGGTGTCAAGCAGTGTGACCGCTACTGGCCAGATGAGGGTGCCTCCCTCTACCACGTATATGAGGT GAACCTGGTGTCGGAGCACATCTGGTGCGAGGACTTTCTGGTGCGGAGCTTCTACCTGAAGAACGTGCAG ACCCAGGAGACGCGCACGCTCACGCAGTTCCACTTCCTCAGCTGGCCGGCAGAGGGCACACCGGCCTCCA CGCGGCCCCTGCTGGACTTCCGCAGGAAGGTGAACAAGTGCTACCGGGGCCGCTCCTGCCCCATCATCGT GCACTGCAGTGATGGTGCGGGGAGGACCGGCACCTACATCCTCATCGACATGGTCCTGAACCGCATGGCA AAAGGAGTGAAGGAGATTGACATCGCTGCCACCCTGGAGCATGTCCGTGACCAGCGGCCTGGCCTTGTCC GCTCTAAGGACCAGTTTGAATTTGCCCTGACAGCCGTGGCGGAGGAAGTGAATGCCATCCTCAAGGCCCT GCCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234994 representing NM_001199764
Red=Cloning site Green=Tags(s) MSQGLSWHDDLTQYVISQEMERIPRLRPPEPRPRDRSGLAPKRPGPAGELLLQDIPTGSAPAAQHRLPQP PVGKGGAGASSSLSPLQAELLPPLLEHLLLPPQPPHPSLSYEPALLQPYLFHQFGSRDGSRVSEGSPGMV SVGPLPKAEAPALFSRTASKGIFGDHPGHSYGDLPGPSPAQLFQDSGLLYLAQELPAPSRARVPRLPEQG SSSRAEDSPEGYEKEGLGDRGEKPASPAVQPDAALQRLAAVLAGYGVELRQLTPEQLSTLLTLLQLLPKG AGRNPGGVVNVGADIKKTMEGPVEGRDTAELPARTSPMPGHPTASPTSSEVQQVPSPVSSEPPKAARPPV TPVLLEKKSPLGQSQPTVAGQPSARPAAEEYGYIVTDQKPLSLAAGVKLLEILAEHVHMSSGSFINISVV GPALTFRIRHNEQNLSLADVTQQAGLVKSELEAQTGLQILQTGVGQREEAAAVLPQTAHSTSPMRSVLLT LVALAGVAGLLVALAVALCVRQHARQQDKERLAALGPEGAHGDTTFEYQDLCRQHMATKSLFNRAEGPPE PSRVSSVSSQFSDAAQASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQ AEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHT IADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQ TQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMA KGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199764 |
| ORF Size | 2667 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199764.2 |
| RefSeq Size | 3570 bp |
| RefSeq ORF | 2670 bp |
| Locus ID | 5798 |
| UniProt ID | Q16849 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Type I diabetes mellitus |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010] |
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