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BRSK2 (NM_001256630) Human Tagged ORF Clone
CAT＃: RG234858
- TrueORF®
BRSK2 (tGFP-tagged) - Homo sapiens BR serine/threonine kinase 2 (BRSK2), transcript variant 4
ORF Plasmid: DDK
"NM_001256630" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C11orf7; PEN11B; SAD1; SADA; STK29 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234858 representing NM_001256630
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCTGAGGGCCACCCCAGCCGATGGGCACGTCCCCGCCGGCCCTGCATCTGTCCTTCCTCCCTCT GCTCCCCAAGAGAGCCCAGGTCTGGCCCAGCGGTGGGCAGGGGAGGGGCCGCACATCACAGAGTGCCAGC TGGCCACACTCCCGGCCCACAGCTGCTCCAGCCGCACCTCCACCTTCCTCAAGGCCAGACCTGGCTCTGC CTGCAGCCCAGCCCAGCAGGTCTGGTGAAGCTGGGGGTTCACTGCGTCACCTGCCAGAAGGTGGCCATCA AGATCGTCAACCGTGAGAAGCTCAGCGAGTCGGTGCTGATGAAGGTGGAGCGGGAGATCGCGATCCTGAA GCTCATTGAGCACCCCCACGTCCTAAAGCTGCACGACGTTTATGAAAACAAAAAATATTTGTACCTGGTG CTAGAACACGTGTCAGGTGGTGAGCTCTTCGACTACCTGGTGAAGAAGGGGAGGCTGACGCCTAAGGAGG CTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGACTTCTGCCACAGCCACTCCATATGCCACAGGGA TCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACAACATCCGCATCGCAGACTTTGGCATGGCGTCC CTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGGGTCCCCCCACTACGCCTGCCCCGAGGTGATCC GGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGGAGCTGCGGCGTCATCCTGTTCGCCTTGCTGGT GGGGGCTCTGCCCTTCGACGATGACAACTTGCGACAGCTGCTGGAGAAGGTGAAGCGGGGCGTGTTCCAC ATGCCGCACTTTATCCCGCCCGACTGCCAGAGTCTGCTACGGGGCATGATCGAGGTGGACGCCGCACGCC GCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGGGGCAAGAATGAGCCCGAACCAGAGCA GCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCAGCCTGGAGGACATCGACCCCGACGTGCTGGAC AGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGACCTGCTGTCCGAGGAGGAGA ACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGGAAAGAAAGGTACCCGAGCCAGGAGGATGAGGA CCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGCGTGTGGACTCCCCGATGCTGAACCGGCACGGC AAGCGGCGGCCAGAACGCAAATCCATGGAGGTGCTCAGCGTGACGGACGGCGGCTCCCCGGTGCCTGCGC GGCGGGCCATTGAGATGGCCCAGCACGGCCAGAGGTCTCGGTCCATCAGCGGTGCCTCCTCAGGCCTTTC CACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACCCCTCACCAAGGGGCAGTCCCCTCCCCACCCCC AAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGCTGGCACGCCCAACCCCACGCCCCCGTCCAGCC CCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAACTCCATCAAGAACAGCTTTCTGGGCTCACCCCG CTTCCACCGCCGGAAACTGCAAGTTCCGACGCCGGAGGAGATGTCCAACCTGACACCAGAGTCGTCCCCA GAGCTGGCGAAGAAGTCCTGGTTTGGGAACTTCATCAGCCTGGAGAAGGAGGAGCAGATCTTCGTGGTCA TCAAAGACAAACCTCTGAGCTCCATCAAGGCTGACATCGTGCACGCCTTCCTGTCGATTCCCAGTCTCAG CCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGTACAAGGCCACGGGGGGGCCAGCCGTGTTCCAG AAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGAGGGTGGGGAGGCGCAGAAGGAGAACGGCATCT ACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGTCGCTTCAAGAGGGTGGTGGAGACCATCCAGGC CCAGCTGCTGAGCACACACGACCCGCCTGCGGCCCAGCACTTGTCAGACACCACTAACTGTATGGAAATG ATGACGGGGCGGCTTTCCAAATGTGACGAGAAGAACGGGCAGGCGGCCCAGGCCCCCAGCACGCCCGCCA AGCGGAGTGCCCACGGCCCACTCGGTGACTCCGCGGCCGCTGGCCCTGGCCCCGGAGGGGACGCCGAGTA CCCAACGGGCAAGGACACGGCCAAGATGGGCCCGCCCACCGCCCGCCGCGAGCAGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234858 representing NM_001256630
Red=Cloning site Green=Tags(s) MSPEGHPSRWARPRRPCICPSSLCSPREPRSGPAVGRGGAAHHRVPAGHTPGPQLLQPHLHLPQGQTWLC LQPSPAGLVKLGVHCVTCQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLV LEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIRIADFGMAS LQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFH MPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLD SMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHG KRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTP KGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRARLNSIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSP ELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQ KPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETIQAQLLSTHDPPAAQHLSDTTNCMEM MTGRLSKCDEKNGQAAQAPSTPAKRSAHGPLGDSAAAGPGPGGDAEYPTGKDTAKMGPPTARREQP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256630 |
| ORF Size | 2298 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256630.1, NP_001243559.1 |
| RefSeq Size | 4520 bp |
| RefSeq ORF | 2301 bp |
| Locus ID | 9024 |
| UniProt ID | Q8IWQ3 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function] |
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