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USP4 (NM_001251877) Human Tagged ORF Clone
CAT＃: RG233728
- TrueORF®
USP4 (tGFP-tagged) - Homo sapiens ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant 3
ORF Plasmid: DDK
"NM_001251877" in other vectors (2)
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CNY 4,370.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | UNP; Unph |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233728 representing NM_001251877
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGACTCAGAAGTCCGAGCTTGGACCCTTAA TGAGGACCACACTCCAACGCGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATTTCCTGGCCCAATAGAC AACTCTGGGCTATTTTCAGATCCTGAGAGTCAGACCTTGAAAGAACACTTAATTGATGAATTGGACTATG TATTGGTCCCTACCGAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCAACCCAT CGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTCGAGGTGTATTTGCTGGAACTG AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAA CCATCGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACACGGCTCTGGAACAAATA CATGAGCAACACCTACGAGCAGTTGAGCAAGCTAGACAACACTGTCCAGGATGCTGGGCTATACCAGGGT CAGGTGCTAGTAATTGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAAAAGTTT CGTTCTTTTTGCCCAGGCTGGAGTGCAATGGCGCGATCTTGGCTCACTGCAACTTCTGCCTCCCGGGTTC AAGCAATTCTCCTGCCTCAGCTTCCCGAGTAGCGCCATCACATCTGGCTAATTTTTTTTTTTTTGAGATG GAGTCTCACTCTGTCACCAAGCTGGAGTGCGGTGGTGCAGTCTCGGCTTACTCCCGGGTTCAAGTGATGC TTCTGCCCCAGCCTCCCGAGTGGCTGGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233728 representing NM_001251877
Red=Cloning site Green=Tags(s) MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID NSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQG QVLVIEPQNEDGTWPRQTLQSKVSFFLPRLECNGAILAHCNFCLPGSSNSPASASRVAPSHLANFFFFEM ESHSVTKLECGGAVSAYSRVQVMLLPQPPEWLG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001251877 |
| ORF Size | 939 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001251877.2 |
| RefSeq Size | 1673 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 7375 |
| UniProt ID | Q13107 |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
Documents
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