PDE4DIP (NM_001198834) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	CMYA2; MMGL
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG231499 representing NM_001198834 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTAATGGATATCGCACTCTGTCCCAGCACCTCAATGACCTGAAGAAGGAGAACTTCAGCCTCAAGC TGCGCATCTACTTCCTGGAGGAGCGCATGCAACAGAAGTATGAGGCCAGCCGGGAGGACATCTACAAGCG GAACATTGAGCTGAAGGTTGAAGTGGAGAGCTTGAAACGAGAACTCCAGGACAAGAAACAGCATCTGGAT AAAACATGGGCTGATGTGGAGAATCTCAACAGTCAGAATGAAGCTGAGCTCCGACGCCAGTTTGAGGAGC GACAGCAGGAGACGGAGCATGTTTATGAGCTCTTGGAGAATAAGATCCAGCTTCTGCAGGAGGAATCCAG GCTAGCAAAGAATGAAGCTGCGCGGATGGCAGCTCTGGTGGAAGCAGAGAAGGAGTGTAACCTGGAGCTC TCAGAGAAACTGAAGGGAGTCACCAAAAACTGGGAAGATGTACCAGGAGACCAGGTCAAGCCCGACCAAT ACACTGAGGCCCTGGCCCAGAGGGACAAGAGAATTGAAGAACTGAATCAGAGCCTGGCTGCCCAGGAGAG GCTTGTAGAACAGCTATCTCGGGAGAAACAACAACTGCTACATCTGTTGGAGGAGCCAACTAGCATGGAA GTGCAGCCCATGACTGAAGAGTTGCTGAAACAACAAAAGCTGAATTCACATGAGACCACTATAACTCAGC AGTCTGTATCTGATTCCCACTTGGCAGAACTCCAGGAAAAAATCCAGCAAACAGAGGCCACCAACAAGAT TCTTCAAGAGAAACTTAATGAAATGAGCTATGAACTAAAGTGTGCTCAGGAGTCGTCTCAAAAGCAAGAT GGTACAATTCAGAACCTCAAGGAAACTCTGAAAAGCAGGGAACGTGAGACTGAGGAGTTGTACCAGGTAA TTGAAGGTCAAAATGACACAATGGCAAAGCTTCGAGAAATGCTGCACCAAAGCCAGCTTGGACAACTTCA CAGCTCAGAGGGTACTTCTCCAGCTCAGCAACAGGTAGCTCTGCTTGATCTTCAGAGTGCTTTATTCTGC AGCCAACTTGAAATACAGAAGCTCCAGAGGGTGGTACGACAGAAAGAGCGCCAACTGGCTGATGCCAAAC AATGTGTGCAATTTGTAGAGGCTGCAGCACACGAGAGTGAACAGCAGAAAGAGGCTTCTTGGAAACATAA CCAGGAATTGCGAAAAGCCTTGCAGCAGCTACAAGAAGAATTGCAGAATAAGAGCCAACAGCTTCGTGCC TGGGAGGCTGAAAAATACAATGAGATTCGAACCCAGGAACAAAACATCCAGCACCTAAACCATAGTCTGA GTCACAAGGAGCAGTTGCTTCAGGAATTTCGGGAGCTCCTACAGTATCGAGATAACTCAGACAAAACCCT TGAAGCAAATGAAATGTTGCTTGAGAAACTTCGCCAGCGAATACATGATAAAGCTGTTGCTCTGGAGCGG GCTATAGATGAAAAATTCTCTGCTCTAGAAGAGAAAGAAAAAGAACTGCGCCAGCTTCGTCTTGCTGTGA GAGAGCGAGATCATGACTTAGAGAGACTGCGCGATGTCCTCTCCTCCAATGAAGCTACTATGCAAAGTAT GGAGAGTCTCCTGAGGGCCAAAGGCCTGGAAGTGGAACAGTTATCTACTACCTGTCAAAACCTCCAGTGG CTGAAAGAAGAAATGGAAACCAAATTTAGCCGTTGGCAGAAGGAACAAGAGAGTATCATTCAGCAGTTAC AGACGTCTCTTCATGATAGGAACAAAGAAGTGGAGGATCTTAGTGCAACACTGCTCTGCAAACTTGGACC AGGGCAGAGTGAGATAGCAGAGGAGCTGTGCCAGCGTCTACAGCGAAAGGAAAGGATGCTGCAGGACCTT CTAAGTGATCGAAATAAACAAGTGCTGGAACATGAAATGGAGATTCAAGGCCTGCTTCAGTCTGTGAGCA CCAGGGAGCAGGAAAGCCAAGCTGCTGCAGAGAAGTTGGTGCAAGCCTTAATGGAAAGAAATTCAGAATT ACAGGCCCTGCGCCAATATTTAGGAGGGAGAGACTCCCTGATGTCCCAAGCACCCATCTCTAACCAACAA GCTGAAGTTACCCCCACTGGCTGTCTTGGAAAACAGACTGATCAAGGTTCAATGCAGATACCTTCCAGAG ATGATAGCACTTCATTGACTGCCAAAGAGGATGTCAGCATACCCAGATCCACATTAGGAGACTTGGACAC AGTTGCAGGGCTGGAAAAAGAACTGAGTAATGCCAAAGAGGAACTTGAACTCATGGCTAAAAAAGAAAGA GAAAGTCAGATGGAACTTTCTGCTCTACAGTCCATGATGGCTGTGCAGGAAGAAGAGCTGCAGGTGCAGG CTGCTGATATGGAGTCTCTGACCAGGAACATACAGATTAAAGAAGATCTCATAAAGGACCTGCAAATGCA ACTGGTTGATCCTGAAGACATACCAGCTATGGAACGCCTGACCCAGGAAGTCTTACTTCTTCGGGAAAAA GTTGCTTCAGTAGAATCCCAGGGTCAAGAAATTTCAGGAAACCGAAGACAACAGTTGCTGCTGATGCTAG AAGGACTAGTAGATGAACGGAGTCGGCTCAATGAGGCCTTACAAGCAGAGAGACAGCTCTATAGCAGTCT GGTGAAGTTCCATGCCCATCCAGAGAGCTCTGAGAGAGACCGAACTCTGCAGGTGGAACTGGAAGGGGCT CAGGTGTTACGCAGTCGGCTAGAAGAAGTTCTTGGAAGAAGCTTGGAGCGCTTAAACAGGCTGGAGACCC TGGCCGCCATTGGAGGTGCAGCTGCAGGGGATGACACCGAAGATACAAGCACTGAGTTCACTGACAGTAT TGAGGAGGAGGCTGCACACCATAGTCACCAGCAACTTGTCAAGGTGGCTTTGGAGAAAAGTCTGGCAACT GTGGAGACCCAGAACCCATCTTTTTCCCCTCCTTCTCCGATGGGAGGGGACAGTAACAGGTGTCTTCAGG AAGAAATGCTCCACCTGAGGGCTGAGTTCCACCAGCACTTAGAAGAGAAGAGGAAAGCTGAGGAGGAACT GAAGGAGCTAAAGGCTCAAATTGAGGAAGCAGGATTCTCCTCAGTGTCCCACATCAGGAACACCATGCTG AGCCTTTGCCTTGAGAATGCGGAGCTGAAAGAGCAGATGGGAGAAGCAATGTCTGATGGATGGGAGATCG AGGAAGACAAGGAGAAGGGCGAGGTGATGGTTGAGACTGTGGTAACCAAAGAGGGTCTGAGTGAGAGTAG CCTTCAGGCTGAGTTCAGAAAGCTCCAGGGAAAACTGAAGAATGCCCACAATATCATCAACCTCCTCAAA GAACAACTTGTGCTGAGTAGCAAGGAAGGGAATAGTAAACTTACTCCAGAGCTCCTTGTGCATCTGACCA GCACCATTGAAAGAATAAACACAGAACTGGTTGGTTCCCCTGGGAAGCACCAACACCAAGAGGAGGGGAA TGTGACTGTGAGGCCTTTCCCCAGACCCCAGAGCCTTGACCTTGGGGCTACCTTCACAGTGGATGCCCAC CAATTGGATAACCAGTCCCAGCCTCGTGACCCTGGGCCTCAGTCAGCGTTTAGCCTACCAGGGTCCACCC AGCACCTGCGCTCCCAGCTGTCACAATGCAAACAACGCTATCAAGATCTCCAGGAGAAGCTGCTGCTATC AGAAGCCACTGTCTTTGCTCAGGCTAACGAGCTGGAGAAATACAGAGTTATGCTTACAGGTGAATCCTTG GTGAAGCAGGACAGCAAGCAGATCCAGGTGGACCTCCAGGACCTGGGCTATGAGACTTGTGGCCGAAGCG AGAATGAGGCTGAACGGGAGGAAACCACCAGTCCTGAGTGTGAGGAGCACAACAGCCTCAAGGAAATGGT CCTGATGGAGGGGCTGTGCTCTGAGCAGGGACGCCGGGGCTCAACACTGGCTAGTTCCTCTGAGAGGAAG CCCTTGGAGAACCAGCTAGGGAAGCAGGAAGAGTTCCGGGTATATGGAAAGTCAGAAAACATCTTGGTCC TACGAAAGGACATCAAAGATCTGAAGGCCCAGCTGCAGAATGCCAACAAGGTCATTCAAAACCTCAAGAG CCGGGTCCGGTCCCTCTCAGTTACAAGTGATTATTCGTCTAGTCTGGAAAGACCCTGGAAGCTGAGAGCT GTTGGCACCTTGGAGGGGTCTTCACCTCATAGTGTCCCTGATGAGGATGAGGGGTGGCTGTCTGATGGCA CTGGGGCTTTCTACTCTCCAGGGCTTCAGGCCAAAAAGGACCTGGAGAGTCTCATCCAGAGAGTATCCCA GCTGGAGGCCCAGCTCCCAAAAAATGGACTAGAAGAGAAGCTGGCTGAGGAGCTGAGATCAGCCTCGTGG CCTGGGAAATATGATTCCCTGATTCAGGATCAGGCCCGGGAACTGTCTTACCTACGGCAAAAAATACGAG AAGGGAGAGGTATTTGTTATCTTATCACCCGGCATGCAAAAGATACAGTAAAATCTTTTGAGGATCTCCT AAGGAGCAATGACATTGACTACTACCTGGGACAGAGCTTCCGGGAGCAACTCGCCCAGGGAAGCCAGCTG ACAGAGAGGCTCACCAGCAAACTCAGCACCAAGGATCATAAAAGTGAGAAAGATCAAGCTGGACTTGAGC CACTGGCCCTCAGGCTCAGCAGGGAGCTGCAGGAGAAGGAGAAAGTGATTGAAGTCCTGCAGGCCAAGCT GGATGCTCGGTCCCTCACACCCTCCAGCAGCCATGCCTTGTCTGACTCCCACCGCTCTCCCAGCAGCACC TCTTTCCTGTCTGATGAACTGGAAGCCTGCTCTGACATGGACATAGTCAGCGAGTACACACACTATGAAG AGAAGAAAGCTTCTCCCAGTCACTCAGATTCCATCCATCATTCGAGTCATTCTGCTGTGTTGTCTTCTAA ACCATCATCAACCAGTGCATCTCAGGGGGCTAAGGCCGAATCCAACAGCAACCCCATCAGCTTGCCAACT CCCCAGAATACCCCCAAGGAGGCCAACCAGGCCCATTCAGGCTTTCATTTTCACTCCATACCCAAGCTGG CTAGCCTTCCTCAGGCACCATTGCCCTCAGCTCCATCCAGCTTCCTGCCTTTCAGCCCCACTGGCCCTCT CCTCCTTGGCTGCTGTGAGACACCAGTGGTCTCCTTGGCTGAGGCTCAGCAGGAGCTACAGATGCTGCAG AAGCAGTTGGGAGAAAGTGCCAGCACTGTTCCTCCTGCTTCCACAGCTACATTGCTGAGCAACGACTTGG AAGCCGACTCTTCCTACTACCTCAACTCTGCCCAGCCTCACTCTCCTCCAAGGGGCACCATAGAACTGGG AAGAATCCTAGAGCCTGGGTACCTGGGCAGCAGTGGCAAGTGGGATGTGATGAGGCCTCAGAAAGGGAGT GTATCTGGGGACCTATCCTCAGGCTCCTCTGTGTACCAGCTTAACTCCAAACCCACAGGGGCTGACCTGC TGGAAGAGCATCTTGGTGAAATCCGGAACCTGCGCCAGCGCCTGGAGGAATCCATCTGCATCAATGACCG CCTACGGGAGCAACTGGAACACCGGCTGACCTCTACTGCTCGTGGAAGGGGATCCACTTCTAACTTCTAC AGTCAGGGCCTGGAGTCCATACCTCAGCTCTGCAATGAGAACAGAGTCCTCAGGGAAGACAATCGAAGAC TTCAGGCTCAACTGAGTCATGTTTCCAGAGAGCACTCCCAGGAAACAGAAAGCCTGAGGGAGGCTCTGCT GTCCTCTCGATCCCACCTTCAAGAGCTGGAAAAGGAGCTGGAGCACCAGAAGGTGGAAAGGCAGCAGCTT TTGGAAGACTTGAGGGAGAAGCAGCAAGAGGTCTTGCATTTCAGGGAGGAACGTCTTTCCCTCCAGGAAA ACGACTCCAGACTGCAGCACAAGCTGGTTCTCCTGCAGCAACAGTGTGAAGAGAAACAGCAGCTCTTTGA GTCCCTCCAGTCAGAGCTACAAATCTACGAGGCACTTTATGGCAATTCCAAGAAGGGGCTGAAAGCTTAC AGCCTGGATGCCTGTCACCAAATCCCTTTGAGCAGTGACCTGAGCCACCTGGTGGCAGAGGTACGAGCTC TGAGAGGGCAGCTGGAGCAGAGCATTCAGGGGAACAATTGTCTGCGACTGCAGCTGCAACAGCAGCTGGA GAGCGGTGCTGGCAAAGCCAGCCTCAGCCCCTCCTCCATTAACCAGAACTTCCCAGCCAGCACTGACCCT GGAAACAAGCAGCTGCTCCTCCAAGATTCAGCTGTGTCCCCTCCAGTCCGGGATGTTGGTATGAATTCCC CAGCTCTGGTCTTCCCCAGCTCTGCTTCCTCTACTCCTGGCTCAGAAACGCCCATAATCAACAGAGCAAA TGGCTTGGGTTTGGATACTTCTCCAGTAATGAAGACCCCTCCCAAGCTAGAGGGTGATGCTACTGATGGC TCCTTTGCCAATAAGCATGGCCGCCATGTCATTGGCCACATTGATGACTACAGTGCCCTAAGACAGCAGA TTGCGGAGGGCAAGCTGCTGGTCAAAAAGATAGTGTCTCTTGTGAGATCAGCGTGCAGCTTCCCTGGCCT TGAAGCCCAAGGCACAGAGGTGCTAGGCAGCAAAGGTATTCATGAGCTTCGGAGCAGCACCAGTGCCCTG CACCATGCCCTAGAGGAGTCAGCTTCCCTCCTCACCATGTTCTGGAGAGCGGCCCTGCCAAGCACCCACA TCCCTGTGCTGCCTGGCAAAGTGGGAGAATCAACAGAAAGGGAACTTCTGGAACTGAGAACCAAAGTATC CAAACAGGAGCGACTCCTTCAGAGCACAACTGAGCATCTGAAGAACGCCAACCAGCAGAAGGAGAGCATG GAACAGTTCATTGTCAGCCAGCTAACCAGAACACATGATGTTTTAAAGAAGGCAAGGACTAACTTAGAGG AACCATGCAAGAAGCGCAGCCACCAGAAGTCCTTAAAACAGCAGGAAAGGTGGGCCTGTCCCCCTTTTGT GCAGCTACCTATCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG231499 representing NM_001198834 Red=Cloning site Green=Tags(s) MSNGYRTLSQHLNDLKKENFSLKLRIYFLEERMQQKYEASREDIYKRNIELKVEVESLKRELQDKKQHLD KTWADVENLNSQNEAELRRQFEERQQETEHVYELLENKIQLLQEESRLAKNEAARMAALVEAEKECNLEL SEKLKGVTKNWEDVPGDQVKPDQYTEALAQRDKRIEELNQSLAAQERLVEQLSREKQQLLHLLEEPTSME VQPMTEELLKQQKLNSHETTITQQSVSDSHLAELQEKIQQTEATNKILQEKLNEMSYELKCAQESSQKQD GTIQNLKETLKSRERETEELYQVIEGQNDTMAKLREMLHQSQLGQLHSSEGTSPAQQQVALLDLQSALFC SQLEIQKLQRVVRQKERQLADAKQCVQFVEAAAHESEQQKEASWKHNQELRKALQQLQEELQNKSQQLRA WEAEKYNEIRTQEQNIQHLNHSLSHKEQLLQEFRELLQYRDNSDKTLEANEMLLEKLRQRIHDKAVALER AIDEKFSALEEKEKELRQLRLAVRERDHDLERLRDVLSSNEATMQSMESLLRAKGLEVEQLSTTCQNLQW LKEEMETKFSRWQKEQESIIQQLQTSLHDRNKEVEDLSATLLCKLGPGQSEIAEELCQRLQRKERMLQDL LSDRNKQVLEHEMEIQGLLQSVSTREQESQAAAEKLVQALMERNSELQALRQYLGGRDSLMSQAPISNQQ AEVTPTGCLGKQTDQGSMQIPSRDDSTSLTAKEDVSIPRSTLGDLDTVAGLEKELSNAKEELELMAKKER ESQMELSALQSMMAVQEEELQVQAADMESLTRNIQIKEDLIKDLQMQLVDPEDIPAMERLTQEVLLLREK VASVESQGQEISGNRRQQLLLMLEGLVDERSRLNEALQAERQLYSSLVKFHAHPESSERDRTLQVELEGA QVLRSRLEEVLGRSLERLNRLETLAAIGGAAAGDDTEDTSTEFTDSIEEEAAHHSHQQLVKVALEKSLAT VETQNPSFSPPSPMGGDSNRCLQEEMLHLRAEFHQHLEEKRKAEEELKELKAQIEEAGFSSVSHIRNTML SLCLENAELKEQMGEAMSDGWEIEEDKEKGEVMVETVVTKEGLSESSLQAEFRKLQGKLKNAHNIINLLK EQLVLSSKEGNSKLTPELLVHLTSTIERINTELVGSPGKHQHQEEGNVTVRPFPRPQSLDLGATFTVDAH QLDNQSQPRDPGPQSAFSLPGSTQHLRSQLSQCKQRYQDLQEKLLLSEATVFAQANELEKYRVMLTGESL VKQDSKQIQVDLQDLGYETCGRSENEAEREETTSPECEEHNSLKEMVLMEGLCSEQGRRGSTLASSSERK PLENQLGKQEEFRVYGKSENILVLRKDIKDLKAQLQNANKVIQNLKSRVRSLSVTSDYSSSLERPWKLRA VGTLEGSSPHSVPDEDEGWLSDGTGAFYSPGLQAKKDLESLIQRVSQLEAQLPKNGLEEKLAEELRSASW PGKYDSLIQDQARELSYLRQKIREGRGICYLITRHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQL TERLTSKLSTKDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSST SFLSDELEACSDMDIVSEYTHYEEKKASPSHSDSIHHSSHSAVLSSKPSSTSASQGAKAESNSNPISLPT PQNTPKEANQAHSGFHFHSIPKLASLPQAPLPSAPSSFLPFSPTGPLLLGCCETPVVSLAEAQQELQMLQ KQLGESASTVPPASTATLLSNDLEADSSYYLNSAQPHSPPRGTIELGRILEPGYLGSSGKWDVMRPQKGS VSGDLSSGSSVYQLNSKPTGADLLEEHLGEIRNLRQRLEESICINDRLREQLEHRLTSTARGRGSTSNFY SQGLESIPQLCNENRVLREDNRRLQAQLSHVSREHSQETESLREALLSSRSHLQELEKELEHQKVERQQL LEDLREKQQEVLHFREERLSLQENDSRLQHKLVLLQQQCEEKQQLFESLQSELQIYEALYGNSKKGLKAY SLDACHQIPLSSDLSHLVAEVRALRGQLEQSIQGNNCLRLQLQQQLESGAGKASLSPSSINQNFPASTDP GNKQLLLQDSAVSPPVRDVGMNSPALVFPSSASSTPGSETPIINRANGLGLDTSPVMKTPPKLEGDATDG SFANKHGRHVIGHIDDYSALRQQIAEGKLLVKKIVSLVRSACSFPGLEAQGTEVLGSKGIHELRSSTSAL HHALEESASLLTMFWRAALPSTHIPVLPGKVGESTERELLELRTKVSKQERLLQSTTEHLKNANQQKESM EQFIVSQLTRTHDVLKKARTNLEEPCKKRSHQKSLKQQERWACPPFVQLPIC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198834
ORF Size	7086 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198834.2, NP_001185763.2
RefSeq Size	8332 bp
RefSeq ORF	7089 bp
Locus ID	9659
UniProt ID	Q5VU43
Gene Summary	The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

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