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DDX3 (DDX3X) (NM_001193417) Human Tagged ORF Clone
CAT＃: RG231353
- TrueORF®
DDX3X (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 3
ORF Plasmid: DDK
"NM_001193417" in other vectors (3)
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CNY 5,040.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAP-Rf; DBX; DDX3; DDX14; HLP2; MRX102; MRXSSB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG231353 representing NM_001193417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCAGTTTGCTGGCCTAGACCTGAACTCTT CAGATAATCAGAGTGGAGGAAGTACAGCCAGCAGTTTCTACGATAAAGACAGTTCAGGGTGGAGTTCTAG CAAAGATAAGGATGCGTATAGCAGTTTTGGATCTCGTAGTGATTCAAGAGGGAAGTCTAGCTTCTTCAGT GATCGTGGAAGTGGATCAAGGGGAAGGTTTGATGATCGTGGACGGAGTGATTACGATGGCATTGGCAGCC GTGGTGACAGAAGTGGCTTTGGCAAATTTGAACGTGGTGGAAACAGTCGCTGGTGTGACAAATCAGATGA AGATGATTGGTCAAAACCACTCCCACCAAGTGAACGCTTGGAACAGGAACTCTTTTCTGGAGGCAACACT GGGATTAATTTTGAGAAATACGATGACATTCCAGTTGAGGCAACAGGCAACAACTGTCCTCCACATATTG AAAGTTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTGAGCTTACTCGTTATACTCGCCC AACTCCAGTGCAAAAGCATGCTATTCCTATTATCAAAGAGAAAAGAGACTTGATGGCTTGTGCCCAAACA GGGTCTGGAAAAACTGCAGCATTTCTGTTGCCCATCTTGAGTCAGATTTATTCAGATGGTCCAGGCGAGG CTTTGAGGGCCATGAAGGAAAATGGAAGGTATGGGCGCCGCAAACAATACCCAATCTCCTTGGTATTAGC ACCAACGAGAGAGTTGGCAGTACAGATCTACGAGGAAGCCAGAAAATTTTCATACCGATCTAGAGTTCGT CCTTGCGTGGTTTATGGTGGTGCCGATATTGGTCAGCAGATTCGAGACTTGGAACGTGGATGCCATTTGT TAGTAGCCACTCCAGGACGTCTAGTGGATATGATGGAAAGAGGAAAGATTGGATTAGACTTTTGCAAATA CTTGGTGTTAGATGAAGCTGATCGGATGTTGGATATGGGGTTTGAGCCTCAGATTCGTAGAATAGTCGAA CAAGATACTATGCCTCCAAAGGGTGTCCGCCACACTATGATGTTTAGTGCTACTTTTCCTAAGGAAATAC AGATGCTGGCTCGTGATTTCTTAGATGAATATATCTTCTTGGCTGTAGGAAGAGTTGGCTCTACCTCTGA AAACATCACACAGAAAGTAGTTTGGGTGGAAGAATCAGACAAACGGTCATTTCTGCTTGACCTCCTAAAT GCAACAGGCAAGGATTCACTGACCTTAGTGTTTGTGGAGACCAAAAAGGGTGCAGATTCTCTGGAGGATT TCTTATACCATGAAGGATACGCATGTACCAGCATCCATGGAGACCGTTCTCAGAGGGATAGAGAAGAGGC CCTTCACCAGTTCCGCTCAGGAAAAAGCCCAATTTTAGTGGCTACAGCAGTAGCAGCAAGAGGACTGGAC ATTTCAAATGTGAAACATGTTATCAATTTTGACTTGCCAAGTGATATTGAAGAATATGTACATCGTATTG GTCGTACGGGACGTGTAGGAAACCTTGGCCTGGCAACCTCATTCTTTAACGAGAGGAACATAAATATTAC TAAGGATTTGTTGGATCTTCTTGTTGAAGCTAAACAAGAAGTGCCGTCTTGGTTAGAAAACATGGCTTAT GAACACCACTACAAGGGTAGCAGTCGTGGACGTTCTAAGAGTAGCAGATTTAGTGGAGGGTTTGGTGCCA GAGACTACCGACAAAGTAGCGGTGCCAGCAGTTCCAGCTTCAGCAGCAGCCGCGCAAGCAGCAGCCGCAG TGGCGGAGGTGGCCACGGTAGCAGCAGAGGATTTGGTGGAGGTGGCTATGGAGGCTTTTACAACAGTGAT GGATATGGAGGAAATTATAACTCCCAGGGGGTTGACTGGTGGGGTAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG231353 representing NM_001193417
Red=Cloning site Green=Tags(s) MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASSFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFS DRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNT GINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQT GSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVR PCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVE QDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLN ATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLD ISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAY EHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSD GYGGNYNSQGVDWWGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001193417 |
| ORF Size | 1938 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001193417.3 |
| RefSeq Size | 5351 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 1654 |
| UniProt ID | O00571 |
| Protein Families | ES Cell Differentiation/IPS |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC231353 | DDX3X (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 3 |
CNY 4,560.00 |
|
| RC231353L3 | Lenti-ORF clone of DDX3X (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 3 |
CNY 6,460.00 |
|
| RC231353L4 | Lenti-ORF clone of DDX3X (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 3 |
CNY 6,460.00 |
