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EVI1 (MECOM) (NM_001163999) Human Tagged ORF Clone
CAT＃: RG229059
- TrueORF®
MECOM (tGFP-tagged) - Human MDS1 and EVI1 complex locus (MECOM), transcript variant 5
ORF Plasmid: DDK
"NM_001163999" in other vectors (4)
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CNY 11,500.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; PRDM3; RUSAT2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG229059 representing NM_001163999
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGCGAAGACTATCCCCATGAAACTATGGCGCCGGATATCCACGAAGAACGGCAATATCGCTGCG AAGACTGTGACCAGCTCTTTGAATCTAAGGCTGAACTAGCAGATCACCAAAAGTTTCCATGCAGTACTCC TCACTCAGCATTTTCAATGGTTGAAGAGGACTTTCAGCAAAAACTCGAAAGCGAGAATGATCTCCAAGAG ATACACACGATCCAGGAGTGTAAGGAATGTGACCAAGTTTTTCCTGATTTGCAAAGCCTGGAGAAACACA TGCTGTCACATACTGAAGAGAGGGAATACAAGTGTGATCAGTGTCCCAAGGCATTTAACTGGAAGTCCAA TTTAATTCGCCACCAGATGTCACATGACAGTGGAAAGCACTATGAATGTGAAAACTGTGCCAAGCAGGTT TTCACGGACCCTAGCAACCTTCAGCGGCACATTCGCTCTCAGCATGTCGGTGCCCGGGCCCATGCATGCC CGGAGTGTGGCAAAACGTTTGCCACTTCGTCGGGCCTCAAACAACACAAGCACATCCACAGCAGTGTGAA GCCCTTTATCTGTGAGGTCTGCCATAAATCCTATACTCAGTTTTCAAACCTTTGCCGTCATAAGCGCATG CATGCTGATTGCAGAACCCAAATCAAGTGCAAAGACTGTGGACAAATGTTCAGCACTACGTCTTCCTTAA ATAAACACAGGAGGTTTTGTGAGGGCAAGAACCATTTTGCGGCAGGTGGATTTTTTGGCCAAGGCATTTC ACTTCCTGGAACCCCAGCTATGGATAAAACGTCCATGGTTAATATGAGTCATGCCAACCCGGGCCTTGCT GACTATTTTGGCGCCAATAGGCATCCTGCTGGTCTTACCTTTCCAACAGCTCCTGGATTTTCTTTTAGCT TCCCTGGTCTGTTTCCTTCCGGCTTGTACCACAGGCCTCCTTTGATACCTGCTAGTTCTCCTGTTAAAGG ACTATCAAGTACTGAACAGACAAACAAAAGTCAAAGTCCCCTCATGACACATCCTCAGATACTGCCAGCT ACACAGGATATTTTGAAGGCACTATCTAAACACCCATCTGTAGGGGACAATAAGCCAGTGGAGCTCCAGC CCGAGAGGTCCTCTGAAGAGAGGCCCTTTGAGAAAATCAGTGACCAGTCAGAGAGTAGTGACCTTGATGA TGTCAGTACACCAAGTGGCAGTGACCTGGAAACAACCTCGGGCTCTGATCTGGAAAGTGACATTGAAAGT GATAAAGAGAAATTTAAAGAAAATGGTAAAATGTTCAAAGACAAAGTAAGCCCTCTTCAGAATCTGGCTT CAATAAATAATAAGAAAGAATACAGCAATCATTCCATTTTCTCACCATCTTTAGAGGAGCAGACTGCGGT GTCAGGAGCTGTGAATGATTCTATAAAGGCTATTGCTTCTATTGCTGAAAAATACTTTGGTTCAACAGGA CTGGTGGGGCTGCAAGACAAAAAAGTTGGAGCTTTACCTTACCCTTCCATGTTTCCCCTCCCATTTTTTC CAGCATTCTCTCAATCAATGTACCCATTTCCTGATAGAGACTTGAGATCGTTACCTTTGAAAATGGAACC CCAATCACCAGGTGAAGTAAAGAAACTGCAGAAGGGCAGCTCTGAGTCCCCCTTTGATCTCACCACTAAG CGAAAGGATGAGAAGCCCTTGACTCCAGTCCCCTCCAAGCCTCCAGTGACACCTGCCACAAGCCAAGACC AGCCCCTGGATCTAAGTATGGGCAGTAGGAGTAGAGCCAGTGGGACAAAGCTGACTGAGCCTCGAAAAAA CCACGTGTTTGGGGGAAAAAAAGGAAGCAACGTCGAATCAAGACCTGCTTCAGATGGTTCCTTGCAGCAT GCAAGACCCACTCCTTTCTTTATGGACCCTATTTACAGAGTAGAGAAAAGAAAACTAACTGACCCACTTG AAGCTTTAAAAGAGAAATACTTGAGGCCTTCTCCAGGATTCTTGTTTCACCCACAAATGTCAGCTATTGA AAACATGGCAGAAAAGCTAGAGAGCTTCAGTGCCCTGAAACCTGAGGCCAGTGAGCTCTTACAGTCAGTG CCCTCTATGTTCAACTTCAGGGCGCCTCCCAATGCCCTGCCAGAGAACCTTCTGCGGAAGGGAAAGGAGC GCTATACCTGCAGATACTGTGGCAAGATTTTTCCAAGGTCTGCAAACCTAACACGGCACTTGAGAACCCA CACAGGAGAGCAGCCTTACAGATGCAAATACTGTGACAGATCATTTAGCATATCTTCTAACTTGCAAAGG CATGTTCGCAACATCCACAATAAAGAGAAGCCATTTAAGTGTCACTTATGTGATAGGTGTTTTGGTCAAC AAACCAATTTAGACAGACACCTAAAGAAACATGAGAATGGGAACATGTCCGGTACAGCAACATCGTCGCC TCATTCTGAACTGGAAAGTACAGGTGCGATTCTGGATGACAAAGAAGATGCTTACTTCACAGAAATTCGA AATTTCATTGGGAACAGCAACCATGGCAGCCAATCTCCCAGGAATGTGGAGGAGAGAATGAATGGCAGTC ATTTTAAAGATGAAAAGGCTTTGGTGACCAGTCAAAATTCAGACTTGCTGGATGATGAAGAAGTTGAAGA TGAGGTGTTGTTAGATGAGGAGGATGAAGACAATGATATTACTGGAAAAACAGGAAAGGAACCAGTGACA AGTAATTTACATGAAGGAAACCCTGAGGATGACTATGAAGAAACCAGTGCCCTGGAGATGAGTTGCAAGA CATCCCCAGTGAGGTATAAAGAGGAAGAATATAAAAGTGGACTTTCTGCTCTAGATCATATAAGGCACTT CACAGATAGCCTCAAAATGAGGAAAATGGAAGATAATCAATATTCTGAAGCTGAGCTGTCTTCTTTTAGT ACTTCCCATGTGCCAGAGGAACTTAAGCAGCCGTTACACAGAAAGTCCAAATCGCAGGCATATGCTATGA TGCTGTCACTGTCTGACAAGGAGTCCCTCCATTCTACATCCCACAGTTCTTCCAACGTGTGGCACAGTAT GGCCAGGGCTGCGGCGGAATCCAGTGCTATCCAGTCCATAAGCCACGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG229059 representing NM_001163999
Red=Cloning site Green=Tags(s) MKSEDYPHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQE IHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQV FTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRM HADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLA DYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPA TQDILKALSKHPSVGDNKPVELQPERSSEERPFEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIES DKEKFKENGKMFKDKVSPLQNLASINNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTG LVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPGEVKKLQKGSSESPFDLTTK RKDEKPLTPVPSKPPVTPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFGGKKGSNVESRPASDGSLQH ARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQMSAIENMAEKLESFSALKPEASELLQSV PSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQR HVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIR NFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVT SNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYSEAELSSFS TSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163999 |
| ORF Size | 3129 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163999.1, NP_001157471.1 |
| RefSeq Size | 4811 bp |
| RefSeq ORF | 3132 bp |
| Locus ID | 2122 |
| UniProt ID | Q13465 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Chronic myeloid leukemia, MAPK signaling pathway, Pathways in cancer |
| Gene Summary | The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229059 | MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 5 |
CNY 10,450.00 |
|
| RC229059L3 | Lenti-ORF clone of MECOM (Myc-DDK-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 5 |
CNY 12,350.00 |
|
| RC229059L4 | Lenti-ORF clone of MECOM (mGFP-tagged)-Human MDS1 and EVI1 complex locus (MECOM), transcript variant 5 |
CNY 12,350.00 |
|
| SC327694 | MECOM (untagged)-Human MDS1 and EVI1 complex locus (MECOM) transcript variant 5 |
CNY 10,450.00 |
