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Semaphorin 7a (SEMA7A) (NM_001146030) Human Tagged ORF Clone
CAT＃: RG228931
- TrueORF®
SEMA7A (tGFP-tagged) - Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 3
ORF Plasmid: DDK
"NM_001146030" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228931 representing NM_001146030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCCTCCTCCGCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCCTGGCCCGCCGGCTC GGTTGGGGCTTCCGCTGCGGCTGCGGCTGCTGCTGCTGCTCTGGGCGGCCGCCGCCTCCGCCCAGGGCCA CCTAAGGAGCGGACCCCGCATCTTCGCCGTCTGGAAAGGCCATGTAGGGCAGGACCGGGTGGACTTTGGC CAGACTGAGCCGCACACGGTGCTTTTCCACGAGCCAGGCAGCTCCTCTGTGTGGGTGGGAGGACGTGGCA AGGTCTACCTCTTTGACTTCCCCGAGGGCAAGAACGCATCTGTGCGCACGGTGAATATCGGCTCCACAAA GGGGTCCTGTCTGGATAAGCGGGACTGCGAGAACTACATCACTCTCCTGGAGAGGCGGAGTGAGGGGCTG CTGGCCTGTGGCACCAACGCCCGGCACCCCAGCTGCTGGAACCTGGTGAATGGCACTGTGGTGCCACTTG GCGAGATGAGAGGCTACGCCCCCTTCAGCCCGGACGAGAACTCCCTGGTTCTGTTTGAAGGGGACGAGGT GTATTCCACCATCCGGAAGCAGGAATACAATGGGAAGATCCCTCGGTTCCGCCGCATCCGGGGCGAGAGT GAGCTGTACACCAGTGATACTGTCATGCAGAACCCACAGTTCATCAAAGCCACCATCGTGCACCAAGACC AGGCTTACGATGACAAGATCTACTACTTCTTCCGAGAGGACAATCCTGACAAGAATCCTGAGGCTCCTCT CAATGTGTCCCGTGTGGCCCAGTTGTGCAGGGGGGACCAGGGTGGGGAAAGTTCACTGTCAGTCTCCAAG TGGAACACTTTTCTGAAAGCCATGCTGGTATGCAGTGATGCTGCCACCAACAAGAACTTCAACAGGCTGC AAGACGTCTTCCTGCTCCCTGACCCCAGCGGCCAGTGGAGGGACACCAGGGTCTATGGTGTTTTCTCCAA CCCCTGGAACTACTCAGCCGTCTGTGTGTATTCCCTCGGTGACATTGACAAGGTCTTCCGTACCTCCTCA CTCAAGGGCTACCACTCAAGCCTTCCCAACCCGCGGCCTGGCAAGTGCCTCCCAGACCAGCAGCCGATAC CCACAGAGACCTTCCAGGTGGCTGACCGTCACCCAGAGGTGGCGCAGAGGGTGGAGCCCATGGGGCCTCT GAAGACGCCATTGTTCCACTCTAAATACCACTACCAGAAAGTGGCCGTCCACCGCATGCAAGCCAGCCAC GGGGAGACCTTTCATGTGCTTTACCTAACTACAGACAGGGGCACTATCCACAAGGTGGTGGAACCGGGGG AGCAGGAGCACAGCTTCGCCTTCAACATCATGGAGATCCAGCCCTTCCGCCGCGCGGCTGCCATCCAGAC CATGTCGCTGGATGCTGAGCGGAGGAAGCTGTATGTGAGCTCCCAGTGGGAGGTGAGCCAGGTGCCCCTG GACCTGTGTGAGGTCTATGGCGGGGGCTGCCACGGTTGCCTCATGTCCCGAGACCCCTACTGCGGCTGGG ACCAAGGCCGCTGCATCTCCATCTACAGCTCCGAACGGTCAGTGCTGCAATCCATTAATCCAGCCGAGCC ACACAAGGAGTGTCCCAACCCCAAACCAGACAAGGCCCCACTGCAGAAGGTTTCCCTGGCCCCAAACTCT CGCTACTACCTGAGCTGCCCCATGGAATCCCGCCACGCCACCTACTCATGGCGCCACAAGGAGAACGTGG AGCAGAGCTGCGAACCTGGTCACCAGAGCCCCAACTGCATCCTGTTCATCGAGAACCTCACGGCGCAGCA GTACGGCCACTACTTCTGCGAGGCCCAGGAGGGCTCCTACTTCCGCGAGGCTCAGCACTGGCAGCTGCTG CCCGAGGACGGCATCATGGCCGAGCACCTGCTGGGTCATGCCTGTGCCCTGGCCGCCTCCCTCTGGCTGG GGGTGCTGCCCACACTCACTCTTGGCTTGCTGGTCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228931 representing NM_001146030
Red=Cloning site Green=Tags(s) MTPPPPGRAAPSAPRARVPGPPARLGLPLRLRLLLLLWAAAASAQGHLRSGPRIFAVWKGHVGQDRVDFG QTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGL LACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGES ELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSK WNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSS LKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASH GETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPL DLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNS RYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLL PEDGIMAEHLLGHACALAASLWLGVLPTLTLGLLVH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146030 |
| ORF Size | 2001 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146030.1, NP_001139502.1 |
| RefSeq Size | 3246 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 8482 |
| UniProt ID | O75326 |
| Protein Pathways | Axon guidance |
| Gene Summary | This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228931 | SEMA7A (Myc-DDK-tagged)-Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 3 |
CNY 7,816.00 |
|
| RC228931L3 | Lenti-ORF clone of SEMA7A (Myc-DDK-tagged)-Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 3 |
CNY 5,890.00 |
|
| RC228931L4 | Lenti-ORF clone of SEMA7A (mGFP-tagged)-Human semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A), transcript variant 3 |
CNY 5,890.00 |
|
| SC327566 | SEMA7A (untagged)-Human semaphorin 7A GPI membrane anchor (John Milton Hagen blood group) (SEMA7A) transcript variant 3 |
CNY 7,220.00 |
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