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Collagen XI alpha 2 (COL11A2) (NM_001163771) Human Tagged ORF Clone
CAT＃: RG228226
- TrueORF®
COL11A2 (tGFP-tagged) - Human collagen, type XI, alpha 2 (COL11A2), transcript variant 4
ORF Plasmid: DDK
"NM_001163771" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DFNA13; DFNB53; FBCG2; HKE5; OSMEDA; OSMEDB; PARP; STL3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG228226 representing NM_001163771
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGTGCAGCCGCTGCCATCGCCTCCTCCTCCTCCTACCTCTGGTGCTGGGGCTGAGCGCGGCCC CAGGCTGGGCAGGTGCACCCCCTGTGGATGTGCTCCGGGCCCTGAGGTTCCCCTCCCTCCCTGATGGTGT CCGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTACCGAGTGGCACGACCTGCCCAGCTCAGTGCA CCCACTCGCCAGCTTTTCCCAGGAGGATTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCC CTGGTCTCCAAGCTCCCCTCCTGACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTGGAGCTGGG CCGACCTGTCCGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTCTTCCGA GGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCTGTGGCTGTGAAGGGCCAGTCTGTCACCCTCA TTGTTGACTGCAAGAAGCGAGTCACCCGGCCTCTCCCCCGAAGTGCTCGTCCAGTATTGGACACCCATGG AGTGATCATCTTTGGTGCCCGTATTCTGGATGAAGAAGTCTTTGAGGGTGATGTCCAGGAGCTGGCCATT GTCCCAGGGGTCCAGGCAGCCTATGAATCATGTGAACAGAAGGAGCTGGAATGCGAGGGGGGCCAGAGGG AAAGACCCCAAAACCAACAGCCTCACAGAGCCCAGAGATCTCCACAGCAGCAACCATCAAGACTTCACAG GCCACAAAATCAGGAACCCCAGAGCCAGGTGAGGGAGCTGGGAGAACCCCCAAGTGCAGCACACCCCAGA GAGGGAAGACACCCAGGCATCTCTCCTCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228226 representing NM_001163771
Red=Cloning site Green=Tags(s) MERCSRCHRLLLLLPLVLGLSAAPGWAGAPPVDVLRALRFPSLPDGVRRAKGICPADVAYRVARPAQLSA PTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVRQLGLELGRPVRFLYEDQTGRPQPPSQPVFR GLSLADGKWHRVAVAVKGQSVTLIVDCKKRVTRPLPRSARPVLDTHGVIIFGARILDEEVFEGDVQELAI VPGVQAAYESCEQKELECEGGQRERPQNQQPHRAQRSPQQQPSRLHRPQNQEPQSQVRELGEPPSAAHPR EGRHPGISPP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163771 |
| ORF Size | 870 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163771.2 |
| RefSeq Size | 1237 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 1302 |
| UniProt ID | P13942 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| Gene Summary | This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC228226 | COL11A2 (Myc-DDK-tagged)-Human collagen, type XI, alpha 2 (COL11A2), transcript variant 4 |
CNY 2,400.00 |
|
| RC228226L3 | Lenti ORF clone of Human collagen, type XI, alpha 2 (COL11A2), transcript variant 4, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC228226L4 | Lenti ORF clone of Human collagen, type XI, alpha 2 (COL11A2), transcript variant 4, mGFP tagged |
CNY 5,890.00 |
|
| SC326861 | COL11A2 (untagged)-Human collagen type XI alpha 2 (COL11A2) transcript variant 4 |
CNY 6,270.00 |
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