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Prickle (PRICKLE1) (NM_001144882) Human Tagged ORF Clone
CAT＃: RG227324
- TrueORF®
PRICKLE1 (tGFP-tagged) - Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 3
ORF Plasmid: DDK
"NM_001144882" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EPM1B; RILP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227324 representing NM_001144882
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTGGAGATGGAGCCCAAGATGAGCAAACTGGCCTTTGGCTGTCAGAGAAGTTCCACATCAGATG ATGACTCTGGCTGTGCATTGGAGGAGTACGCCTGGGTCCCCCCGGGCCTGAGACCAGAGCAGATCCAGCT CTATTTTGCTTGCTTACCAGAGGAAAAAGTTCCTTACGTTAACAGCCCCGGAGAGAAGCATCGGATTAAA CAGCTTTTGTACCAGTTACCACCACATGATAATGAGGTACGGTATTGCCAGTCTTTGAGTGAAGAGGAGA AAAAAGAGTTGCAGGTGTTCAGTGCTCAGCGGAAGAAAGAAGCACTGGGAAGAGGAACAATTAAGCTTCT GTCCAGAGCAGTCATGCATGCTGTGTGTGAGCAGTGTGGTTTGAAGATAAATGGAGGTGAAGTTGCAGTG TTCGCCTCCCGTGCGGGCCCTGGTGTGTGCTGGCACCCATCCTGTTTTGTCTGTTTCACGTGTAATGAGC TGCTGGTCGACCTCATCTATTTTTATCAGGATGGAAAAATTCACTGTGGCAGGCACCATGCAGAACTGCT CAAACCACGGTGCTCAGCATGTGACGAGATAATTTTTGCTGATGAGTGCACAGAAGCTGAGGGTCGCCAT TGGCACATGAAACACTTCTGCTGCCTTGAGTGTGAAACGGTCCTGGGAGGACAGAGGTATATCATGAAGG ACGGCCGCCCCTTCTGCTGTGGCTGTTTTGAGTCTCTCTATGCGGAGTACTGTGAAACCTGTGGGGAACA TATTGGTGTGGACCATGCACAGATGACCTATGACGGGCAGCACTGGCACGCCACGGAAGCCTGCTTTTCT TGTGCCCAGTGTAAAGCCTCTTTGTTGGGATGTCCCTTCCTTCCCAAACAGGGTCAGATTTACTGCTCAA AAACGTGCAGTCTTGGTGAAGACGTCCATGCCTCTGATTCTTCCGACTCTGCATTTCAGTCAGCTCGATC AAGAGACTCCCGAAGAAGTGTCCGAATGGGCAAGAGCAGCCGGTCAGCAGATCAGTGTAGACAGTCTCTC CTCTTATCGCCTGCTCTGAACTACAAGTTTCCTGGCCTCTCAGGCAATGCTGATGACACCCTTTCTCGAA AATTGGATGATCTGAGTCTCTCCAGACAAGGAACAAGTTTTGCCAGTGAAGAATTTTGGAAAGGCAGAGT AGAGCAGGAAACTCCAGAAGACCCTGAAGAATGGGCTGATCATGAAGATTATATGACGCAGCTCCTCCTC AAGTTTGGTGATAAAAGCCTCTTTCAGCCACAGCCCAATGAGATGGATATTCGAGCCAGTGAGCACTGGA TATCTGATAACATGGTTAAAAGTAAGACCGAGTTAAAGCAAAATAACCAGAGCCTTGCAAGTAAAAAATA CCAGTCTGATATGTACTGGGCACAGTCACAAGATGGACTGGGCGATTCTGCTTATGGCAGCCACCCAGGC CCTGCAAGCAGTAGAAGGCTTCAGGAATTGGAACTGGACCATGGGGCTTCAGGGTATAATCATGATGAAA CACAGTGGTATGAAGATTCCCTGGAGTGTCTGTCAGACCTGAAACCAGAGCAAAGTGTTCGGGATTCGAT GGATTCTTTGGCATTGTCCAATATCACAGGGGCTTCGGTGGATGGAGAAAACAAGCCAAGGCCATCATTG TATTCTCTGCAAAATTTTGAGGAGATGGAAACAGAAGATTGTGAGAAGATGAGCAATATGGGAACTTTGA ACTCTTCCATGCTGCACAGGAGTGCAGAGTCCTTAAAGAGTCTAAGTTCAGAGTTGTGTCCAGAGAAAAT CCTGCCTGAAGAGAAGCCAGTACATCTGCCAGTGCTCAGAAGGTCCAAGTCTCAATCCAGACCCCAGCAG GTCAAGTTTTCTGATGATGTCATTGACAATGGGAACTATGACATTGAAATCCGGCAGCCTCCGATGAGTG AAAGGACTCGGAGACGCGTCTACAATTTTGAAGAGAGGGGATCCAGGTCTCATCACCACCGCCGCCGGAG AAGTAGAAAGTCCCGCTCCGACAATGCCCTGAATCTTGTTACAGAAAGAAAATACTCTCCCAAGGACAGA CTGCGGCTGTACACCCCCGATAACTATGAGAAATTTATACAGAATAAAAGTGCCCGGGAGATCCAAGCAT ACATCCAGAATGCTGATCTCTACGGACAGTACGCCCATGCCACTTCCGATTATGGCCTGCAGAACCCAGG AATGAATCGGTTTCTGGGACTCTACGGCGAGGATGATGATTCCTGGTGTTCTTCCTCCTCCTCCTCTTCC GACTCGGAAGAAGAAGGATATTTTCTTGGACAACCAATCCCTCAACCCCGGCCACAGAGATTTGCCTACT ATACAGATGACCTTTCTAGTCCACCATCTGCACTTCCCACCCCTCAGTTTGGTCAGAGGACAACAAAATC CAAGAAGAAAAAGGGACACAAGGGCAAAAATTGTATTATTTCT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227324 representing NM_001144882
Red=Cloning site Green=Tags(s) MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIK QLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAVMHAVCEQCGLKINGGEVAV FASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRH WHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFS CAQCKASLLGCPFLPKQGQIYCSKTCSLGEDVHASDSSDSAFQSARSRDSRRSVRMGKSSRSADQCRQSL LLSPALNYKFPGLSGNADDTLSRKLDDLSLSRQGTSFASEEFWKGRVEQETPEDPEEWADHEDYMTQLLL KFGDKSLFQPQPNEMDIRASEHWISDNMVKSKTELKQNNQSLASKKYQSDMYWAQSQDGLGDSAYGSHPG PASSRRLQELELDHGASGYNHDETQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGENKPRPSL YSLQNFEEMETEDCEKMSNMGTLNSSMLHRSAESLKSLSSELCPEKILPEEKPVHLPVLRRSKSQSRPQQ VKFSDDVIDNGNYDIEIRQPPMSERTRRRVYNFEERGSRSHHHRRRRSRKSRSDNALNLVTERKYSPKDR LRLYTPDNYEKFIQNKSAREIQAYIQNADLYGQYAHATSDYGLQNPGMNRFLGLYGEDDDSWCSSSSSSS DSEEEGYFLGQPIPQPRPQRFAYYTDDLSSPPSALPTPQFGQRTTKSKKKKGHKGKNCIIS SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001144882 |
| ORF Size | 2493 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001144882.2 |
| RefSeq Size | 4253 bp |
| RefSeq ORF | 2496 bp |
| Locus ID | 144165 |
| UniProt ID | Q96MT3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Wnt signaling pathway |
| Gene Summary | This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227324 | PRICKLE1 (Myc-DDK-tagged)-Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 3 |
CNY 5,800.00 |
|
| RC227324L3 | Lenti-ORF clone of PRICKLE1 (Myc-DDK-tagged)-Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 3 |
CNY 7,700.00 |
|
| RC227324L4 | Lenti-ORF clone of PRICKLE1 (mGFP-tagged)-Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 3 |
CNY 7,700.00 |
|
| SC325349 | PRICKLE1 (untagged)-Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 3 |
CNY 7,220.00 |
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