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ASAP3 (NM_001143778) Human Tagged ORF Clone
CAT＃: RG226781
- TrueORF®
ASAP3 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2
ORF Plasmid: DDK
"NM_001143778" in other vectors (4)
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CNY 10,360.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACAP4; CENTB6; DDEFL1; UPLC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226781 representing NM_001143778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAGCAGTTCAGCGTCGCCGAGTTCCTGGCCGTCACCGCGGAGGACCTCAGCTCCCCGGCTGGGG CCGCCGCCTTCGCCGCCAAGATGCCCCGGTACCGAGGGGCGGCGCTGGCGCGGGAGGAGATCTTGGAAGG AGACCAAGCCATCCTGCAGAGAATAAAGAAGGCTGTGCGGGCAATCCATAGCTCCGGCCTTGGCCATGTG GAGAATGAAGAGCAGTACCGAGAGGCCGTGGAATCCTTAGGCAACAGCCACCTGTCCCAGAACAGCCATG AGCTGTCCACAGGCTTCCTAAACTTGGCCGTGTTCACCCGCGAGGTTGCTGCGCTCTTCAAGAACCTGAT TCAGAACTTGAACAACATTGTCTCTTTCCCCCTGGACAGTCTGATGAAGGGGCAGCTGAGGGACGGTCGA CAGGCTTCTCTTTCCTTGGGGTCTAGGGCCAAGCTGGAGAAGGAGCGCGATCGGGCCAGGGTGACAGGAG GGATCCCTGGGGAGGTGGCCCAGGACATGCAGAGAGAGCGGCGCATCTTCCAGCTGCACATGTGTGAGTA TCTGCTCAAAGCCGGGGAGAGCCAGATGAAGCAAGGTCCTGACTTCCTTCAGAGCCTCATCAAGTTCTTC CACGCCCAGCACAACTTTTTCCAAGATGGCTGGAAGGCTGCCCAGAGCCTGTTCCCCTTCATCGAGAAGC TGGCGGCCTCAGTACATGCACTCCATCAGGCCCAGGAGGACGAGCTACAGAAGCTGACCCAGCTCCGGGA CTCCCTCCGAGGGACACTGCAGCTTGAGAGCAGAGAGGAACACCTGAGCCGGAAGAACTCAGGATGTGGC TATAGCATCCACCAGCACCAAGGCAACAAGCAGTTTGGGACGGAGAAAGTGGGCTTTCTATACAAGAAAA GTGACGGAATTCGAAGAGTCTGGCAGAAAAGGAAGTGTGGAGTCAAGTATGGCTGCCTGACCATCTCACA CAGCACGATAAACCGGCCCCCGGTGAAGCTGACCCTGCTGACGTGCCAAGTGAGGCCAAACCCTGAGGAG AAAAAGTGCTTCGACCTGGTGACCCACAACCGGACGTACCACTTTCAGGCAGAGGACGAGCACGAGTGTG AGGCGTGGGTGTCAGTGTTGCAGAACAGCAAGGACGAAGCCCTGAGCAGCGCCTTCCTCGGGGAGCCCAG CGCTGGCCCGGGGTCCTGGGGGTCCGCCGGCCATGATGGGGAGCCGCACGACCTCACAAAGCTGCTCATC GCGGAGGTGAAGAGCAGGCCTGGGAATAGCCAGTGCTGCGACTGCGGGGCTGCAGACCCCACGTGGCTCA GCACCAACCTGGGCGTGCTCACCTGCATCCAGTGCTCGGGCGTCCACCGCGAACTGGGCGTGCGCTTTTC GCGCATGCAGTCACTCACCTTGGACCTGCTGGGCCCCTCCGAGTTGTTGCTGGCCTTGAACATGGGAAAC ACGAGCTTCAATGAGGTCATGGAGGCCCAGCTACCCTCACACGGCGGCCCTAAACCCTCAGCTGAGAGTG ACATGGGCACCCGCAGGGACTACATTATGGCCAAGTATGTGGAGCATAGGTTTGCACGCCGGTGCACACC TGAGCCTCAGCGACTCTGGACAGCCATTTGCAACAGGGACCTCCTGTCGGTACTGGAGGCCTTTGCCAAT GGGCAGGACTTTGGACAGCCGCTGCCAGGGCCTGATGCACAGGCACCTGAAGAACTCGTCTTGCATTTGG CTGTCAAAGTCGCCAACCAGGCTTCCCTGCCTCTGGTGGATTTCATCATCCAGAACGGTGGTCACCTGGA TGCCAAGGCTGCTGACGGGAACACGGCTCTGCACTACGCAGCACTCTACAACCAGCCCGACTGCCTCAAG CTGCTGCTGAAGGGGAGAGCTTTGGTTGGCACAGTAAATGAAGCAGGCGAGACAGCTCTGGACATAGCCA GGAAGAAGCACCACAAGGAGTGTGAGGAGCTGCTGGAGCAGGCCCAGGCGGGGACCTTTGCCTTCCCTCT ACATGTGGACTACTCCTGGGTAATTTCCACAGAGCCTGGCTCTGACAGTGAGGAGGATGAGGAAGAGAAG CGCTGCTTGCTGAAGCTCCCGGCCCAGGCTCACTGGGCCAGTGGGAGGCTGGACATCAGCAACAAGACCT ATGAGACTGTCGCCAGCCTGGGAGCAGCCACCCCTCAGGGCGAGAGTGAGGACTGTCCCCCGCCCTTGCC AGTCAAAAACTCTTCTCGGACTTTGGTCCAAGGGTGTGCAAGACATGCCAGTGGAGATCGTTCTGAAGTC TCCAGCCTGAGTTCAGAGGCCCCTGAGACCCCTGAGAGCCTGGGCAGTCCAGCCTCCTCCTCCAGTCTGA TGAGCCCCTTGGAACCTGGGGATCCCAGCCAAGCCCCACCCAACTCTGAAGAGGGCCTCCGAGAGCCCCC AGGCACCTCCAGACCCAGCCTGACATCCGGGACCACCCCTTCGGAGATGTACCTCCCCGTCAGATTCAGC TCCGAGAGCACTCGCTCCTATCGGCGGGGGGCGCGGAGCCCTGAAGATGGTCCCTCAGCCAGGCAGCCTC TGCCCAGAAGGAACGTGCCGGTTGGCATCACTGAAGGAGATGGCTCAAGGACTGGGAGTCTCCCAGCAAG TTCTGTGCAACTTTTGCAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226781 representing NM_001143778
Red=Cloning site Green=Tags(s) MPEQFSVAEFLAVTAEDLSSPAGAAAFAAKMPRYRGAALAREEILEGDQAILQRIKKAVRAIHSSGLGHV ENEEQYREAVESLGNSHLSQNSHELSTGFLNLAVFTREVAALFKNLIQNLNNIVSFPLDSLMKGQLRDGR QASLSLGSRAKLEKERDRARVTGGIPGEVAQDMQRERRIFQLHMCEYLLKAGESQMKQGPDFLQSLIKFF HAQHNFFQDGWKAAQSLFPFIEKLAASVHALHQAQEDELQKLTQLRDSLRGTLQLESREEHLSRKNSGCG YSIHQHQGNKQFGTEKVGFLYKKSDGIRRVWQKRKCGVKYGCLTISHSTINRPPVKLTLLTCQVRPNPEE KKCFDLVTHNRTYHFQAEDEHECEAWVSVLQNSKDEALSSAFLGEPSAGPGSWGSAGHDGEPHDLTKLLI AEVKSRPGNSQCCDCGAADPTWLSTNLGVLTCIQCSGVHRELGVRFSRMQSLTLDLLGPSELLLALNMGN TSFNEVMEAQLPSHGGPKPSAESDMGTRRDYIMAKYVEHRFARRCTPEPQRLWTAICNRDLLSVLEAFAN GQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLK LLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEK RCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEV SSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFS SESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001143778 |
| ORF Size | 2682 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001143778.2 |
| RefSeq Size | 4172 bp |
| RefSeq ORF | 2685 bp |
| Locus ID | 55616 |
| UniProt ID | Q8TDY4 |
| Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
| Gene Summary | This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226781 | ASAP3 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2 |
CNY 9,410.00 |
|
| RC226781L3 | Lenti-ORF clone of ASAP3 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2 |
CNY 11,310.00 |
|
| RC226781L4 | Lenti-ORF clone of ASAP3 (mGFP-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2 |
CNY 11,310.00 |
|
| SC326276 | ASAP3 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), transcript variant 2 |
CNY 17,200.00 |
