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Proteasome Activator Subunit 4 (PSME4) (NM_014614) Human Tagged ORF Clone
CAT＃: RG222965
- TrueORF®
PSME4 (tGFP-tagged) - Human proteasome (prosome, macropain) activator subunit 4 (PSME4)
ORF Plasmid: DDK
"NM_014614" in other vectors (3)
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CNY 20,240.00
货期*
3周
规格
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经常一起买 (5)
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PA200 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222965 representing NM_014614
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGCCGAGCGGGCGGGAGTCGGAGAGCCCCCGGAGCCGGGCGGGCGTCCCGAGCCGGGCCCGC GGGGCTTCGTCCCGCAGAAGGAGATCGTCTACAACAAGCTGCTGCCCTACGCGGAGCGGCTAGACGCCGA GTCCGACTTGCAGCTGGCCCAGATCAAATGCAACCTGGGCCGGGCCGTGCAGCTCCAAGAGCTGTGGCCC GGGGGCCTCTTCTGGACCAGGAAACTCTCCACATATATTCGACTTTATGGGAGAAAATTTAGCAAAGAAG ATCATGTTCTTTTTATTAAGTTATTGTATGAGCTGGTATCAATTCCAAAACTGGAAATCAGCATGATGCA GGGATTTGCCCGCCTTTTGATCAACTTGTTAAAGAAAAAGGAACTTCTTTCAAGAGCTGATTTGGAGTTA CCCTGGAGACCACTTTATGACATGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTAGGATTAAATT GGTTTCCTAATTCTGTAGAAAATATTCTCAAAACACTCGTGAAAAGCTGCCGACCATATTTTCCAGCAGA TGCCACCGCTGAGATGCTAGAAGAATGGCGACCTTTAATGTGCCCTTTTGATGTAACCATGCAAAAGGCC ATCACTTATTTTGAAATATTTCTTCCTACCTCCCTTCCTCCAGAACTTCATCATAAAGGTTTTAAACTTT GGTTTGATGAATTAATTGGCCTTTGGGTTTCAGTGCAAAATCTCCCACAATGGGAGGGGCAACTAGTAAA TCTCTTTGCTCGATTGGCTACAGATAATATAGGGTACATAGATTGGGATCCATATGTACCAAAGATATTT ACAAGAATTCTGAGAAGCTTGAACCTCCCAGTGGGAAGCAGTCAAGTGTTAGTCCCAAGATTTTTAACAA ATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCCATGATGGGTGGACCAAGTAAGCTAGTGCA AAAACACTTAGCTGGTTTGTTTAACAGCATCACATCTTTTTACCATCCTTCAAATAATGGGCGCTGGCTG AACAAGTTAATGAAACTACTTCAGCGGTTGCCAAACAGTGTTGTTAGAAGATTGCATCGTGAAAGATACA AGAAGCCCTCTTGGTTAACTCCTGTGCCTGATAGCCACAAGCTTACTGATCAAGATGTTACAGACTTTGT ACAATGCATTATTCAGCCTGTCCTCTTGGCTATGTTTAGCAAAACCGGTAGTCTAGAAGCAGCCCAGGCT TTGCAGAATCTTGCACTCATGAGACCTGAATTGGTAATACCCCCTGTACTTGAAAGAACATATCCTGCAT TAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAGTTGTGTAATTGGAGTAGCCCGCAGTTT GGTATCAGGAGGCAGATGGTTTCCTGAAGGTCCTACACATATGCTACCTCTGTTGATGAGAGCATTGCCT GGGGTGGATCCAAATGACTTTAGTAAATGCATGATCACATTCCAGTTCATAGCAACATTTTCTACTCTGG TGCCTTTAGTAGATTGTTCATCTGTACTACAAGAAAGAAATGACCTCACAGAAGTGGAACGAGAACTTTG TTCAGCCACAGCTGAATTTGAGGATTTCGTCTTACAGTTTATGGACAGATGTTTTGGACTTATAGAAAGT AGCACATTGGAGCAAACAAGAGAAGAGACAGAAACTGAGAAAATGACACACTTGGAGAGTTTGGTCGAAT TAGGTCTGTCTTCTACGTTTAGTACAATCCTCACCCAATGTTCCAAAGAAATATTTATGGTGGCCCTTCA GAAGGTTTTTAATTTTTCTACTTCACATATATTTGAAACAAGAGTAGCAGGTCGCATGGTGGCAGACATG TGCCGCGCTGCTGTAAAGTGCTGCCCAGAAGAATCTTTGAAGCTCTTTGTTCCCCACTGCTGCAGTGTTA TAACTCAGCTTACAATGAATGATGATGTATTAAATGATGAAGAGCTAGACAAGGAATTACTATGGAATCT TCAACTTTTGTCTGAGATTACTCGAGTGGATGGAAGGAAGTTGCTTCTTTATAGGGAGCAGCTTGTAAAG ATTCTCCAAAGAACCCTACATTTAACCTGTAAGCAGGGTTACACTCTGTCTTGTAACCTTTTGCATCATC TTCTCCGTTCTACCACACTTATCTACCCTACAGAATACTGCAGTGTGCCAGGTGGCTTTGACAAGCCTCC TTCTGAATACTTTCCTATCAAGGACTGGGGCAAACCCGGGGACTTGTGGAATCTGGGAATCCAGTGGCAT GTTCCTTCTTCAGAAGAAGTGTCTTTTGCCTTTTATCTTTTGGACTCCTTTCTTCAGCCTGAGCTCGTCA AACTCCAGCATTGTGGGGATGGAAAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGT GCACAACTGTTTAATTGGCTCTGGAAACCTCCTACCTCCGTTGAAAGGAGAGCCAGTTACTAACTTAGTA CCAAGTATGGTGTCCTTGGAAGAGACAAAGTTGTATACTGGACTTGAATATGATCTGTCTCGAGAGAACC ACCGAGAAGTAATTGCTACAGTTATAAGGAAACTTCTTAACCACATACTTGATAATTCAGAAGATGATAC TAAGTCATTGTTTCTTATTATAAAGATTATTGGAGACCTTTTACAATTCCAAGGATCTCACAAGCATGAA TTTGACTCCCGATGGAAAAGCTTCAACTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGGAAAAAAC AACATATCAGAGCACTGTTGATTGATAGAGTAATGTTACAGCATGAGCTACGGACACTAACTGTTGAGGG TTGTGAATACAAAAAGATACATCAAGATATGATCAGAGATCTTCTTCGTTTATCTACAAGTTCATACAGT CAGGTGAGAAATAAGGCTCAGCAAACATTTTTTGCTGCCTTGGGAGCATATAACTTCTGTTGCAGAGATA TCATTCCCTTGGTTTTGGAGTTCTTAAGGCCTGATAGACAAGGTGTTACACAGCAACAATTCAAGGGTGC CTTGTACTGTCTCCTTGGAAATCACAGTGGTGTGTGCTTGGCAAACCTTCATGATTGGGACTGTATTGTA CAGACGTGGCCAGCGATTGTTTCTTCAGGGCTTAGCCAAGCAATGTCCCTGGAAAAGCCATCAATAGTGA GATTGTTTGATGATCTTGCAGAAAAGATTCATAGGCAGTATGAAACAATTGGCTTGGACTTCACAATTCC AAAGTCATGTGTTGAAATAGCGGAATTACTTCAACAGTCAAAAAACCCCTCTATCAACCAGATATTGCTT AGCCCAGAAAAAATTAAGGAAGGAATTAAACGCCAACAGGAAAAGAATGCCGATGCCCTAAGGAACTATG AAAATTTGGTAGACACCTTGCTAGATGGTGTGGAGCAAAGAAACCTGCCCTGGAAATTTGAACATATAGG CATTGGGCTTCTGTCTCTACTGCTGAGAGATGACCGAGTGTTGCCTCTTCGTGCCATACGGTTTTTTGTT GAGAATCTCAACCATGATGCAATTGTAGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATCCTTAAAC AGCTAAAAAGAACCCACAAAAAGCTGACCATTAACCCCTGTGAAATCAGTGGATGCCCTAAACCCACCCA AATTATTGCTGGTGATAGGCCTGATAATCATTGGTTGCATTATGACAGCAAAACTATACCAAGAACTAAA AAAGAATGGGAGTCAAGTTGCTTTGTGGAAAAAACTCACTGGGGATACTACACCTGGCCAAAGAATATGG TTGTTTATGCTGGTGTGGAAGAGCAGCCTAAGCTTGGCAGAAGCAGGGAGGATATGACAGAGGCAGAACA GATTATATTTGATCATTTTTCTGATCCTAAATTTGTTGAGCAGTTAATTACTTTTCTATCATTAGAAGAC AGAAAAGGAAAAGATAAGTTTAATCCACGACGTTTTTGCCTCTTTAAGGGTATATTCAGGAATTTTGATG ATGCCTTCCTGCCAGTTCTGAAGCCCCATTTAGAACATTTGGTTGCAGATTCACATGAAAGCACCCAGCG ATGTGTTGCAGAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCACTGGACATTTGAAAAGGTGGAGAAG CTTTGGGAGCTTCTGTGCCCTCTGCTTAGAACAGCACTGTCCAATATTACCGTAGAAACTTATAATGACT GGGGAGCTTGTATAGCAACATCCTGTGAAAGCAGAGATCCCCGGAAACTTCACTGGCTTTTTGAACTGCT GTTGGAATCACCATTGAGTGGTGAAGGAGGATCCTTTGTAGATGCATGTCGACTTTATGTACTACAAGGT GGCCTTGCCCAGCAAGAATGGAGAGTGCCTGAACTATTGCACAGACTACTGAAGTACTTGGAACCCAAAC TCACCCAGGTTTACAAAAATGTCAGAGAAAGAATAGGAAGTGTGCTGACCTACATATTCATGATAGATGT ATCTTTGCCAAATACCACACCAACCATATCGCCTCATGTCCCTGAGTTTACTGCTCGAATTCTGGAGAAA TTGAAACCTCTCATGGATGTGGATGAAGAAATTCAGAACCATGTTATGGAAGAAAATGGAATTGGTGAAG AAGATGAGCGAACTCAGGGCATTAAACTCTTGAAAACCATATTGAAATGGCTGATGGCAAGTGCAGGAAG ATCCTTTTCTACAGCAGTTACAGAACAACTTCAGCTTCTACCTTTGTTTTTCAAGATTGCCCCAGTGGAA AATGACAATAGCTACGATGAACTGAAAAGAGATGCAAAGTTATGTTTATCATTAATGTCTCAGGGGTTGC TTTACCCTCATCAAGTGCCTTTGGTACTTCAGGTGCTAAAACAAACAGCAAGAAGCAGTTCTTGGCATGC ACGATACACAGTACTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA GATGCAGTTAAAGATATCAGGTGGCTGGTTATAAGTCTTTTGGAGGACGAACAACTGGAGGTTCGAGAAA TGGCTGCTACTACCTTAAGCGGTCTGCTACAGTGTAACTTTCTTACCATGGACAGTCCTATGCAGATTCA TTTTGAGCAACTTTGCAAAACAAAACTACCTAAGAAAAGAAAGCGAGACCCTGGTTCTGTAGGAGATACC ATTCCTTCTGCAGAGTTGGTCAAACGCCATGCTGGGGTGCTAGGACTTGGTGCATGTGTTCTTTCTAGTC CTTACGATGTTCCCACCTGGATGCCCCAGCTCCTCATGAATCTCAGTGCACATCTAAATGATCCTCAGCC TATTGAGATGACTGTAAAAAAAACCTTATCCAATTTCCGAAGGACTCACCATGACAACTGGCAGGAACAT AAACAGCAATTCACTGATGACCAACTGCTTGTTCTCACCGATCTTCTTGTGTCACCATGCTATTATGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222965 representing NM_014614
Red=Cloning site Green=Tags(s) MEPAERAGVGEPPEPGGRPEPGPRGFVPQKEIVYNKLLPYAERLDAESDLQLAQIKCNLGRAVQLQELWP GGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRADLEL PWRPLYDMVERILYSKTEHLGLNWFPNSVENILKTLVKSCRPYFPADATAEMLEEWRPLMCPFDVTMQKA ITYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIF TRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWL NKLMKLLQRLPNSVVRRLHRERYKKPSWLTPVPDSHKLTDQDVTDFVQCIIQPVLLAMFSKTGSLEAAQA LQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLSCVIGVARSLVSGGRWFPEGPTHMLPLLMRALP GVDPNDFSKCMITFQFIATFSTLVPLVDCSSVLQERNDLTEVERELCSATAEFEDFVLQFMDRCFGLIES STLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKEIFMVALQKVFNFSTSHIFETRVAGRMVADM CRAAVKCCPEESLKLFVPHCCSVITQLTMNDDVLNDEELDKELLWNLQLLSEITRVDGRKLLLYREQLVK ILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFDKPPSEYFPIKDWGKPGDLWNLGIQWH VPSSEEVSFAFYLLDSFLQPELVKLQHCGDGKLEMSRDDILQSLTIVHNCLIGSGNLLPPLKGEPVTNLV PSMVSLEETKLYTGLEYDLSRENHREVIATVIRKLLNHILDNSEDDTKSLFLIIKIIGDLLQFQGSHKHE FDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYS QVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRQGVTQQQFKGALYCLLGNHSGVCLANLHDWDCIV QTWPAIVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCVEIAELLQQSKNPSINQILL SPEKIKEGIKRQQEKNADALRNYENLVDTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFV ENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPCEISGCPKPTQIIAGDRPDNHWLHYDSKTIPRTK KEWESSCFVEKTHWGYYTWPKNMVVYAGVEEQPKLGRSREDMTEAEQIIFDHFSDPKFVEQLITFLSLED RKGKDKFNPRRFCLFKGIFRNFDDAFLPVLKPHLEHLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEK LWELLCPLLRTALSNITVETYNDWGACIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQG GLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTTPTISPHVPEFTARILEK LKPLMDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVTEQLQLLPLFFKIAPVE NDNSYDELKRDAKLCLSLMSQGLLYPHQVPLVLQVLKQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNE DAVKDIRWLVISLLEDEQLEVREMAATTLSGLLQCNFLTMDSPMQIHFEQLCKTKLPKKRKRDPGSVGDT IPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEH KQQFTDDQLLVLTDLLVSPCYYA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014614 |
| ORF Size | 5529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014614.3 |
| RefSeq Size | 7101 bp |
| RefSeq ORF | 5532 bp |
| Locus ID | 23198 |
| UniProt ID | Q14997 |
| Protein Pathways | Proteasome |
| Gene Summary | Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222965 | PSME4 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) activator subunit 4 (PSME4) |
CNY 12,392.00 |
|
| RC222965L1 | Lenti ORF clone of Human proteasome (prosome, macropain) activator subunit 4 (PSME4), Myc-DDK-tagged |
CNY 14,792.00 |
|
| SC318681 | PSME4 (untagged)-Human proteasome (prosome, macropain) activator subunit 4 (PSME4) |
CNY 35,530.00 |
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