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Proteasome Activator Subunit 4 (PSME4) (NM_014614) Human Tagged Lenti ORF Clone
CAT＃: RC222965L1
- LentiORF®
Lenti ORF clone of Human proteasome (prosome, macropain) activator subunit 4 (PSME4), Myc-DDK-tagged
"NM_014614" in other vectors (3)
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CNY 14,792.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PA200 |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC222965).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014614 |
| ORF Size | 5529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014614.2 |
| RefSeq Size | 7101 bp |
| RefSeq ORF | 5532 bp |
| Locus ID | 23198 |
| UniProt ID | Q14997 |
| Protein Pathways | Proteasome |
| MW | 211.2 kDa |
| Gene Summary | Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222965 | PSME4 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) activator subunit 4 (PSME4) |
CNY 12,392.00 |
|
| RG222965 | PSME4 (tGFP-tagged) - Human proteasome (prosome, macropain) activator subunit 4 (PSME4) |
CNY 20,240.00 |
|
| SC318681 | PSME4 (untagged)-Human proteasome (prosome, macropain) activator subunit 4 (PSME4) |
CNY 35,530.00 |
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