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Raptor (RPTOR) (NM_020761) Human Tagged ORF Clone
CAT＃: RG222281
- TrueORF®
RPTOR (tGFP-tagged) - Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1
ORF Plasmid: DDK
"NM_020761" in other vectors (6)
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CNY 15,304.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KOG1; Mip1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG222281 representing NM_020761
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCGAAATGCTGCAATCGCCTCTTCTGGGCCTGGGGGAGGAAGATGAGGCTGATCTTACAGACT GGAACCTACCTTTGGCTTTTATGAAAAAGAGGCACTGTGAGAAAATTGAAGGCTCCAAATCCTTAGCTCA GAGCTGGAGGATGAAGGATCGGATGAAGACAGTCAGTGTTGCCTTAGTTTTGTGCCTGAATGTTGGTGTG GACCCTCCCGATGTGGTGAAGACCACGCCCTGTGCACGCTTGGAATGCTGGATCGATCCTCTGTCGATGG GTCCTCAGAAAGCTCTGGAAACCATCGGTGCAAATTTACAGAAGCAGTACGAGAACTGGCAGCCAAGGGC CCGGTACAAGCAGAGCCTTGACCCAACTGTGGATGAAGTCAAGAAGCTCTGCACGTCCTTACGTCGCAAC GCCAAGGAGGAGCGAGTCCTCTTTCACTACAATGGCCACGGGGTGCCCCGGCCCACAGTCAACGGGGAGG TCTGGGTCTTCAACAAGAACTACACGCAGTACATCCCTCTGTCCATATATGACCTGCAGACGTGGATGGG CAGCCCGTCGATCTTCGTCTACGACTGCTCCAATGCTGGCTTGATCGTCAAGTCCTTCAAGCAGTTCGCA CTACAGCGGGAGCAGGAGCTGGAGGTAGCTGCAATCAACCCAAATCACCCTCTTGCTCAGATGCCTTTGC CTCCGTCGATGAAAAACTGCATCCAGCTGGCAGCCTGCGAGGCCACCGAGCTGCTGCCCATGATCCCCGA CCTCCCGGCTGACCTATTCACCTCCTGCCTCACCACCCCCATCAAGATCGCCCTGCGCTGGTTTTGCATG CAGAAATGTGTCAGTCTGGTGCCTGGCGTCACACTGGATTTGATAGAAAAGATCCCTGGCCGCCTGAACG ACAGGAGGACGCCCCTGGGTGAACTGAACTGGATCTTCACAGCCATCACAGACACCATCGCGTGGAACGT GCTCCCCCGGGATCTCTTCCAAAAGCTCTTCAGACAGGACTTGCTGGTGGCTAGTCTGTTTCGAAATTTT TTATTGGCGGAAAGGATTATGAGGTCGTATAACTGCACTCCCGTCAGCAGCCCGCGTCTGCCGCCCACGT ACATGCACGCCATGTGGCAAGCCTGGGACCTGGCTGTTGACATCTGTCTGTCTCAGCTGCCGACGATCAT CGAGGAAGGCACTGCGTTTCGGCACAGCCCGTTCTTCGCCGAGCAGCTGACCGCATTCCAGGTGTGGCTC ACCATGGGTGTGGAGAACCGAAACCCACCCGAACAGCTGCCCATCGTCCTGCAGGTGCTGTTAAGCCAAG TGCACCGGCTGAGAGCATTGGACTTGCTTGGAAGATTTTTGGACCTGGGTCCCTGGGCAGTGAGCCTGGC CTTGTCTGTCGGCATCTTCCCCTACGTGCTGAAGCTGCTCCAGAGCTCGGCCCGAGAGCTGCGGCCACTT CTCGTTTTCATCTGGGCCAAGATCCTCGCAGTGGACAGCTCGTGCCAAGCGGACCTCGTGAAGGACAACG GCCACAAGTACTTCCTGTCGGTCCTGGCGGACCCCTACATGCCAGCTGAACACCGGACCATGACGGCTTT CATTCTCGCCGTGATCGTCAACAGCTATCACACGGGGCAGGAAGCCTGCCTTCAGGGAAACCTCATTGCC ATCTGCCTGGAGCAGCTCAACGACCCGCACCCCTTGCTGCGCCAGTGGGTGGCCATCTGCCTCGGCAGGA TCTGGCAGAACTTCGACTCGGCGAGGTGGTGCGGCGTGAGGGACAGCGCTCATGAGAAGCTCTACAGCCT CCTCTCCGACCCCATTCCCGAGGTCCGCTGCGCAGCGGTCTTCGCCCTTGGCACGTTCGTGGGCAACTCT GCAGAGAGGACGGACCACTCCACCACCATCGACCACAACGTGGCCATGATGCTGGCCCAGCTGGTCAGCG ACGGGAGCCCCATGGTCCGGAAGGAGCTGGTGGTGGCTCTGAGTCATCTTGTGGTTCAGTATGAAAGCAA TTTCTGCACCGTGGCCCTGCAGTTCATAGAAGAGGAAAAGAACTACGCCTTGCCTTCTCCAGCAACCACA GAGGGAGGGAGTTTGACCCCAGTGCGAGACAGCCCGTGCACCCCCAGACTTCGTTCTGTGAGCTCCTATG GAAACATCCGTGCTGTCGCCACAGCCAGGAGCCTCAACAAATCTTTGCAGAACCTGAGTTTGACAGAGGA ATCTGGTGGCGCGGTGGCGTTCTCCCCCGGAAACCTCAGCACCAGCAGCAGCGCCAGCAGCACCCTGGGC AGCCCCGAGAATGAGGAGCATATCCTGTCCTTCGAGACCATCGACAAGATGCGCCGCGCCAGCTCCTACT CCTCCCTCAACTCCCTCATCGGAGTTTCCTTTAACAGTGTTTACACTCAGATTTGGAGAGTCCTGCTGCA CCTGGCTGCTGACCCCTATCCAGAGGTCTCGGACGTGGCCATGAAAGTACTCAACAGCATCGCCTACAAG GCCACCGTGAACGCCCGGCCGCAGCGCGTCCTGGACACCTCCTCCCTCACGCAGTCGGCCCCCGCCAGCC CCACCAACAAGGGCGTGCACATCCACCAGGCGGGGGGCTCCCCTCCGGCGTCCAGCACCAGCAGCTCCAG CCTGACCAACGATGTGGCCAAGCAGCCGGTCAGCCGAGACTTGCCTTCTGGCCGGCCGGGCACCACAGGC CCCGCTGGGGCGCAGTACACCCCTCACTCCCACCAGTTCCCCCGGACACGGAAGATGTTCGACAAGGGCC CAGAGCAGACTGCGGACGACGCGGACGATGCTGCTGGACACAAAAGTTTCATCTCCGCCACGGTGCAGAC GGGGTTCTGCGACTGGAGCGCCCGCTATTTTGCCCAGCCCGTCATGAAGATCCCAGAAGAGCACGACCTG GAGAGTCAGATCCGCAAGGAGCGGGAGTGGCGGTTCCTGCGAAACAGCCGTGTCAGGAGGCAGGCCCAGC AAGTCATTCAGAAGGGCATTACGAGATTGGACGACCAAATATTTCTGAACAGGAACCCCGGCGTCCCCTC TGTGGTGAAATTCCACCCCTTCACGCCGTGCATCGCCGTAGCCGACAAGGACAGCATCTGCTTTTGGGAC TGGGAGAAAGGGGAGAAGCTGGATTATTTCCACAATGGGAACCCTCGGTACACGAGGGTCACTGCCATGG AGTATCTGAACGGCCAGGACTGCTCGCTTCTGCTGACGGCCACAGACGATGGTGCCATCAGGGTCTGGAA GAATTTTGCTGATTTGGAAAAGAACCCAGAGATGGTGACCGCGTGGCAGGGGCTCTCGGACATGCTGCCA ACGACGCGAGGAGCTGGGATGGTGGTGGACTGGGAGCAGGAGACCGGCCTCCTCATGAGCTCAGGAGACG TGCGGATCGTCCGGATCTGGGACACAGACCGTGAGATGAAGGTGCAGGACATCCCTACGGGCGCAGACAG CTGTGTGACGAGTCTGTCCTGTGATTCCCACCGCTCACTCATCGTGGCTGGCCTCGGTGACGGCTCCATC CGCGTCTACGACAGAAGGATGGCACTCAGCGAATGCCGTGTCATGACGTACCGGGAGCACACAGCCTGGG TGGTGAAGGCCTCCCTGCAGAAGCGTCCCGACGGCCACATCGTGAGTGTGAGCGTCAATGGAGATGTGCG CATCTTTGATCCCCGGATGCCTGAGTCGGTAAATGTGCTTCAGATCGTGAAGGGGCTGACGGCCCTGGAC ATCCACCCCCAGGCGGACCTGATCGCATGTGGCTCCGTCAATCAGTTCACCGCCATCTACAACAGCAGCG GAGAGCTCATCAACAACATCAAGTACTACGACGGCTTCATGGGCCAGCGGGTCGGCGCCATCAGCTGCCT GGCCTTCCACCCGCACTGGCCTCACCTGGCCGTGGGAAGCAACGACTACTACATCTCCGTGTACTCGGTG GAGAAGCGTGTCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222281 representing NM_020761
Red=Cloning site Green=Tags(s) MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSVALVLCLNVGV DPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYKQSLDPTVDEVKKLCTSLRRN AKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIYDLQTWMGSPSIFVYDCSNAGLIVKSFKQFA LQREQELEVAAINPNHPLAQMPLPPSMKNCIQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCM QKCVSLVPGVTLDLIEKIPGRLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNF LLAERIMRSYNCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWL TMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLLQSSARELRPL LVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILAVIVNSYHTGQEACLQGNLIA ICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSAHEKLYSLLSDPIPEVRCAAVFALGTFVGNS AERTDHSTTIDHNVAMMLAQLVSDGSPMVRKELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATT EGGSLTPVRDSPCTPRLRSVSSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLG SPENEEHILSFETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYK ATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRDLPSGRPGTTG PAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFCDWSARYFAQPVMKIPEEHDL ESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLNRNPGVPSVVKFHPFTPCIAVADKDSICFWD WEKGEKLDYFHNGNPRYTRVTAMEYLNGQDCSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLP TTRGAGMVVDWEQETGLLMSSGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSI RVYDRRMALSECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALD IHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGSNDYYISVYSV EKRVR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020761 |
| ORF Size | 4005 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020761.1, NP_065812.1 |
| RefSeq Size | 6431 bp |
| RefSeq ORF | 4008 bp |
| Locus ID | 57521 |
| UniProt ID | Q8N122 |
| Domains | WD40 |
| Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
| Gene Summary | This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222281 | RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1 |
CNY 13,704.00 |
|
| RC222281L1 | Lenti-ORF clone of RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1 |
CNY 16,104.00 |
|
| RC222281L2 | Lenti-ORF clone of RPTOR (mGFP-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1 |
CNY 15,200.00 |
|
| RC222281L3 | Lenti-ORF clone of RPTOR (Myc-DDK-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1 |
CNY 15,200.00 |
|
| RC222281L4 | Lenti-ORF clone of RPTOR (mGFP-tagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1 |
CNY 16,104.00 |
|
| SC309134 | RPTOR (untagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1 |
CNY 13,712.00 |
