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PSD (NM_002779) Human Tagged ORF Clone
CAT＃: RG221887
- TrueORF®
PSD (tGFP-tagged) - Human pleckstrin and Sec7 domain containing (PSD)
ORF Plasmid: DDK
"NM_002779" in other vectors (4)
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CNY 11,310.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EFA6; EFA6A; PSD1; TYL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221887 representing NM_002779
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGGTGCCATGCGCTTCTGCTCGGAAGGCGACTGTGCCATCTCCCCACCACGATGCCCACGCC GCTGGCTCCCCGAAGGCCCGGTGCCCCAGAGCCCCCCAGCCAGCATGTATGGCAGCACAGGCTCCTTGCT ACGGCGAGTGGCAGGTCCAGGTCCTCGAGGCCGGGAACTGGGACGTGTGACAGCACCCTGTACACCTCTG CGTGGCCCCCCCTCACCCCGTGTTGCTCCCTCACCCTGGGCACCCTCTTCACCCACTGGGCAGCCCCCAC CCGGGGCCCAGAGCTCTGTGGTCATCTTCCGCTTTGTGGAGAAGGCCAGTGTGAGGCCACTGAATGGGCT ACCTGCTCCAGGGGGCTTGAGTCGGAGCTGGGATCTGGGTGGGGTTTCTCCTCCCAGGCCCACCCCAGCC CTTGGGCCTGGCTCCAACCGGAAGTTACGGCTGGAAGCATCCACATCAGACCCACTCCCCGCCAGAGGAG GCTCGGCCCTACCTGGCAGCCGGAACCTTGTACATGGGCCGCCAGCCCCACCCCAGGTTGGAGCAGATGG CCTTTACTCCTCTCTCCCCAATGGGCTGGGGGGCCCCCCTGAGCGCCTGGCCACACTCTTCGGAGGACCT GCTGACACTGGATTCCTGAACCAGGGGGATACCTGGTCCTCCCCCCGGGAAGTCTCCTCTCATGCCCAGA GAATCGCTAGAGCCAAATGGGAATTCTTCTATGGCTCCTTGGACCCCCCCAGCTCAGGTGCTAAGCCCCC AGAGCAGGCCCCCCCATCTCCACCTGGGGTGGGCTCAAGGCAGGGCTCTGGGGTGGCTGTGGGGCGAGCA GCCAAGTACTCCGAGACAGACCTGGACACGGTGCCCCTGAGGTGCTACCGCGAGACTGACATCGATGAGG TGCTGGCTGAGCGGGAGGAGGCCGACTCGGCCATCGAAAGTCAGCCCAGCTCTGAGGGCCCACCAGGCAC TGCCTACCCACCTGCCCCACGGCCCGGCCCACTCCCTGGCCCTCATCCCAGCCTCGGCAGTGGCAATGAG GATGAGGACGACGATGAGGCAGGTGGGGAAGAAGATGTGGACGACGAGGTGTTTGAGGCCTCTGAAGGGG CCCGGCCAGGGAGCCGGATGCCTCTCAAGTCACCTGTGCCCTTTCTACCTGGGACGAGCCCCTCGGCTGA TGGGCCTGACTCTTTCAGTTGTGTGTTCGAAGCCATCCTGGAGTCACACCGGGCCAAAGGCACCTCCTAT ACCAGCCTCGCCTCGCTGGAGGCCTTGGCCTCACCTGGCCCAACCCAGAGCCCCTTCTTCACCTTTGAGC TGCCTCCCCAACCCCCTGCACCCCGGCCCGACCCACCAGCTCCCGCCCCACTTGCCCCTCTTGAACCGGA TTCTGGTACCAGCTCTGCTGCTGATGGTCCTTGGACACAGAGAGGGGAGGAGGAGGAGGCAGAGGCCAGA GCCAAGCTGGCCCCAGGGAGGGAGCCCCCTAGTCCCTGCCACTCAGAGGACAGCCTTGGGCTGGGGGCAG CACCCCTTGGCAGCGAACCACCCCTGAGCCAGCTGGTGTCCGACTCAGACTCAGAGCTGGACAGCACAGA GCGGCTGGCCCTGGGAAGCACAGACACCTTGTCCAATGGGCAGAAAGCGGACCTGGAGGCTGCGCAGCGC CTGGCCAAGAGGCTGTACCGACTAGATGGCTTCAGGAAGGCCGATGTGGCCCGGCACCTGGGCAAGAACA ATGACTTCAGCAAACTGGTGGCTGGGGAGTACCTCAAGTTCTTTGTCTTCACGGGCATGACTCTGGACCA AGCTCTCAGGGTGTTTCTGAAGGAGCTGGCCTTAATGGGTGAGACCCAGGAACGAGAGCGCGTGCTGGCC CACTTCTCCCAGCGATACTTCCAGTGCAATCCTGAAGCCCTGTCCTCAGAGGACGGCGCCCACACGCTGA CCTGTGCGCTCATGCTGCTCAACACGGATCTCCACGGCCATAACATCGGGAAGCGCATGACCTGCGGGGA CTTCATCGGGAACCTGGAGGGCCTCAATGATGGCGGCGACTTCCCTAGGGAGCTGCTCAAGGCCTTGTAC AGCTCCATCAAGAATGAGAAGCTGCAGTGGGCCATAGACGAGGAGGAGCTGAGACGCTCTCTGTCTGAGT TGGCCGACCCCAACCCCAAGGTCATCAAGCGGATCAGCGGGGGCAGTGGCAGTGGCTCCAGCCCTTTCCT GGACCTGACTCCCGAGCCTGGGGCTGCCGTCTACAAGCACGGGGCCCTGGTGCGAAAGGTGCACGCAGAC CCTGACTGCAGGAAGACACCTCGGGGCAAGCGGGGCTGGAAGAGCTTCCACGGGATCCTCAAGGGCATGA TCCTCTACCTGCAGAAGGAGGAGTACAAGCCTGGGAAGGCCCTTTCAGAGACGGAGCTCAAGAATGCCAT CAGCATCCACCATGCCCTGGCCACTCGTGCCAGTGACTACAGCAAGAGGCCCCACGTCTTCTACCTGCGC ACAGCTGACTGGCGGGTCTTCCTCTTCCAGGCCCCGAGCCTGGAGCAGATGCAGTCCTGGATCACTCGCA TCAATGTAGTAGCCGCTATGTTCTCTGCGCCCCCCTTCCCAGCTGCTGTTAGCTCCCAAAAGAAGTTCAG CCGCCCTCTCCTGCCCAGCGCTGCCACCCGCCTCTCCCAGGAGGAGCAGGTGCGGACCCACGAGGCCAAG CTGAAGGCCATGGCAAGTGAGCTGCGGGAGCACCGGGCCGCCCAGCTGGGCAAGAAGGGCCGGGGCAAGG AGGCTGAAGAGCAGCGGCAGAAGGAGGCCTACCTGGAGTTTGAGAAATCCCGCTACAGCACCTATGCAGC GCTGCTTCGGGTCAAGCTGAAGGCAGGCAGTGAGGAGCTGGATGCAGTGGAGGCAGCACTGGCCCAGGCC GGGAGCACAGAGGATGGACTCCCTCCTTCTCACTCCAGTCCCTCCCTGCAGCCCAAACCCTCCAGCCAGC CCCGGGCTCAGCGTCACAGCTCAGAGCCTCGGCCAGGGGCAGGCAGTGGGCGGCGGAAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221887 representing NM_002779
Red=Cloning site Green=Tags(s) MAQGAMRFCSEGDCAISPPRCPRRWLPEGPVPQSPPASMYGSTGSLLRRVAGPGPRGRELGRVTAPCTPL RGPPSPRVAPSPWAPSSPTGQPPPGAQSSVVIFRFVEKASVRPLNGLPAPGGLSRSWDLGGVSPPRPTPA LGPGSNRKLRLEASTSDPLPARGGSALPGSRNLVHGPPAPPQVGADGLYSSLPNGLGGPPERLATLFGGP ADTGFLNQGDTWSSPREVSSHAQRIARAKWEFFYGSLDPPSSGAKPPEQAPPSPPGVGSRQGSGVAVGRA AKYSETDLDTVPLRCYRETDIDEVLAEREEADSAIESQPSSEGPPGTAYPPAPRPGPLPGPHPSLGSGNE DEDDDEAGGEEDVDDEVFEASEGARPGSRMPLKSPVPFLPGTSPSADGPDSFSCVFEAILESHRAKGTSY TSLASLEALASPGPTQSPFFTFELPPQPPAPRPDPPAPAPLAPLEPDSGTSSAADGPWTQRGEEEEAEAR AKLAPGREPPSPCHSEDSLGLGAAPLGSEPPLSQLVSDSDSELDSTERLALGSTDTLSNGQKADLEAAQR LAKRLYRLDGFRKADVARHLGKNNDFSKLVAGEYLKFFVFTGMTLDQALRVFLKELALMGETQERERVLA HFSQRYFQCNPEALSSEDGAHTLTCALMLLNTDLHGHNIGKRMTCGDFIGNLEGLNDGGDFPRELLKALY SSIKNEKLQWAIDEEELRRSLSELADPNPKVIKRISGGSGSGSSPFLDLTPEPGAAVYKHGALVRKVHAD PDCRKTPRGKRGWKSFHGILKGMILYLQKEEYKPGKALSETELKNAISIHHALATRASDYSKRPHVFYLR TADWRVFLFQAPSLEQMQSWITRINVVAAMFSAPPFPAAVSSQKKFSRPLLPSAATRLSQEEQVRTHEAK LKAMASELREHRAAQLGKKGRGKEAEEQRQKEAYLEFEKSRYSTYAALLRVKLKAGSEELDAVEAALAQA GSTEDGLPPSHSSPSLQPKPSSQPRAQRHSSEPRPGAGSGRRKP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002779 |
| ORF Size | 3072 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002779.5 |
| RefSeq Size | 4185 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 5662 |
| UniProt ID | A5PKW4 |
| Protein Pathways | Endocytosis |
| Gene Summary | This gene encodes a Plekstrin homology and SEC7 domains-containing protein that functions as a guanine nucleotide exchange factor. The encoded protein regulates signal transduction by activating ADP-ribosylation factor 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221887 | PSD (Myc-DDK-tagged)-Human pleckstrin and Sec7 domain containing (PSD) |
CNY 7,128.00 |
|
| RC221887L3 | Lenti-ORF clone of PSD (Myc-DDK-tagged)-Human pleckstrin and Sec7 domain containing (PSD) |
CNY 12,160.00 |
|
| RC221887L4 | Lenti-ORF clone of PSD (mGFP-tagged)-Human pleckstrin and Sec7 domain containing (PSD) |
CNY 12,160.00 |
|
| SC303242 | PSD (untagged)-Human pleckstrin and Sec7 domain containing (PSD) |
CNY 10,450.00 |
