United States
Germany
Japan
United Kingdom
China
MARK1 (NM_018650) Human Tagged ORF Clone
CAT＃: RG221556
- TrueORF®
MARK1 (tGFP-tagged) - Human MAP/microtubule affinity-regulating kinase 1 (MARK1)
ORF Plasmid: DDK
"NM_018650" in other vectors (8)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MARK; Par-1c; Par1c |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221556 representing NM_018650
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCCGGACGCCATTGCCGACGGTGAACGAGCGGGACACGGAAAATCATACATCTGTGGATGGAT ATACTGAACCACACATCCAGCCTACCAAGTCGAGTAGCAGACAGAACATCCCCCGGTGTAGAAACTCCAT TACGTCAGCAACAGATGAACAGCCTCACATTGGAAATTACCGTTTACAAAAAACAATAGGGAAGGGAAAT TTTGCCAAAGTCAAATTGGCAAGACACGTTCTAACTGGTAGAGAGGTTGCTGTGAAAATAATAGACAAAA CTCAGCTAAATCCTACCAGTCTACAAAAGTTATTTCGAGAAGTACGAATAATGAAGATACTGAATCATCC TAATATAGTAAAATTGTTTGAAGTTATTGAAACAGAGAAGACTCTCTATTTAGTCATGGAATACGCGAGT GGGGGTGAAGTATTTGATTACTTAGTTGCCCATGGAAGAATGAAAGAGAAAGAGGCCCGTGCAAAATTTA GGCAGATTGTATCTGCTGTACAGTATTGTCATCAAAAGTACATTGTTCACCGTGATCTTAAGGCTGAAAA CCTTCTCCTTGATGGTGATATGAATATTAAAATTGCTGACTTTGGTTTTAGTAATGAATTTACAGTTGGG AACAAATTGGACACATTTTGTGGAAGCCCACCCTATGCTGCTCCCGAGCTTTTCCAAGGAAAGAAGTATG ATGGGCCTGAAGTGGATGTGTGGAGTCTGGGCGTCATTCTCTATACATTAGTCAGTGGCTCCTTGCCTTT CGATGGCCAGAATTTAAAGGAACTGCGAGAGCGAGTTTTACGAGGGAAGTACCGTATTCCCTTCTATATG TCCACAGACTGTGAAAATCTTCTGAAGAAATTATTAGTCCTGAATCCAATAAAGAGAGGCAGCTTGGAAC AAATAATGAAAGATCGATGGATGAATGTTGGTCATGAAGAGGAAGAACTAAAGCCATATACTGAGCCTGA TCCGGATTTCAATGACACAAAAAGAATAGACATTATGGTCACCATGGGCTTTGCACGAGATGAAATAAAT GATGCCTTAATAAATCAGAAGTATGATGAAGTTATGGCTACTTATATTCTTCTAGGTAGAAAACCACCTG AATTTGAAGGTGGTGAATCGTTATCCAGTGGAAACTTGTGTCAGAGGTCCCGGCCCAGTAGTGACTTAAA CAACAGCACTCTTCAGTCCCCTGCTCACCTGAAGGTCCAGAGAAGTATCTCAGCAAATCAGAAGCAGCGG CGTTTCAGTGATCATGCTGGTCCATCCATTCCTCCTGCTGTATCATATACCAAAAGACCTCAGGCTAACA GTGTGGAAAGTGAACAGAAAGAGGAGTGGGACAAAGATGTGGCTCGAAAACTTGGCAGCACAACAGTTGG ATCAAAAAGCGAGATGACTGCAAGCCCTCTTGTAGGGCCAGAGAGGAAAAAATCTTCAACTATTCCAAGT AACAATGTGTATTCTGGAGGTAGCATGGCAAGAAGGAATACATATGTCTGTGAAAGGACCACAGATCGAT ACGTAGCATTGCAGAATGGAAAAGACAGCAGCCTTACGGAGATGTCTGTGAGTAGCATATCTTCTGCAGG CTCTTCTGTGGCCTCTGCTGTCCCCTCAGCACGACCCCGCCACCAGAAGTCCATGTCCACTTCTGGTCAT CCTATTAAAGTCACACTGCCAACCATTAAAGACGGCTCTGAAGCTTACCGGCCTGGTACAACCCAGAGAG TGCCTGCTGCTTCCCCATCTGCTCACAGTATTAGTACTGCGACTCCAGACCGGACCCGTTTTCCCCGAGG GAGCTCAAGCCGAAGCACTTTCCATGGTGAACAGCTCCGGGAGCGACGCAGCGTTGCTTATAATGGGCCA CCTGCTTCACCATCCCATGAAACGGGTGCATTTGCACATGCCAGAAGGGGAACGTCAACTGGTATAATAA GCAAAATCACATCCAAATTTGTTCGCAGGGATCCAAGTGAAGGCGAAGCCAGTGGCAGAACCGACACCTC AAGAAGTACATCAGGGGAACCAAAAGAAAGAGACAAGGAAGAGGGTAAAGATTCTAAGCCGCGTTCTTTG CGGTTCACATGGAGTATGAAGACCACTAGTTCAATGGACCCTAATGACATGATGAGAGAAATCCGAAAAG TGTTAGATGCAAATAACTGTGATTATGAGCAAAAAGAGAGATTTTTGCTTTTCTGTGTCCATGGAGACGC TAGACAGGATAGCCTCGTGCAGTGGGAGATGGAAGTCTGCAAGTTGCCACGACTGTCACTTAATGGGGTT CGCTTCAAGCGAATATCTGGGACATCTATTGCCTTTAAGAACATTGCATCAAAAATAGCAAATGAGCTTA AGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221556 representing NM_018650
Red=Cloning site Green=Tags(s) MSARTPLPTVNERDTENHTSVDGYTEPHIQPTKSSSRQNIPRCRNSITSATDEQPHIGNYRLQKTIGKGN FAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYAS GGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVG NKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYM STDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVTMGFARDEIN DALINQKYDEVMATYILLGRKPPEFEGGESLSSGNLCQRSRPSSDLNNSTLQSPAHLKVQRSISANQKQR RFSDHAGPSIPPAVSYTKRPQANSVESEQKEEWDKDVARKLGSTTVGSKSEMTASPLVGPERKKSSTIPS NNVYSGGSMARRNTYVCERTTDRYVALQNGKDSSLTEMSVSSISSAGSSVASAVPSARPRHQKSMSTSGH PIKVTLPTIKDGSEAYRPGTTQRVPAASPSAHSISTATPDRTRFPRGSSSRSTFHGEQLRERRSVAYNGP PASPSHETGAFAHARRGTSTGIISKITSKFVRRDPSEGEASGRTDTSRSTSGEPKERDKEEGKDSKPRSL RFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLFCVHGDARQDSLVQWEMEVCKLPRLSLNGV RFKRISGTSIAFKNIASKIANELKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018650 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018650.5 |
| RefSeq Size | 5293 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 4139 |
| UniProt ID | Q9P0L2 |
| Domains | UBA, pkinase, TyrKc, KA1, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | Serine/threonine-protein kinase (PubMed:23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Chemical genetic approach identifies microtubule affinity-regulating kinase 1 as a leucine-rich repeat kinase 2 substrate.
,null,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
,PubMed ID 25854701
[MARK1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221556 | MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 3,990.00 |
|
| RC221556L1 | Lenti-ORF clone of MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,890.00 |
|
| RC221556L2 | Lenti-ORF clone of MARK1 (mGFP-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,890.00 |
|
| RC221556L3 | Lenti-ORF clone of MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,890.00 |
|
| RC221556L4 | Lenti-ORF clone of MARK1 (mGFP-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,890.00 |
|
| SC113408 | MARK1 (untagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,560.00 |
|
| SC318279 | MARK1 (untagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,832.00 |
|
| SC323553 | MARK1 (untagged)-Kinase deficient mutant (K89M) of Human MAP/microtubule affinity-regulating kinase 1 (MARK1) |
CNY 5,560.00 |
